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Literature summary extracted from

  • Magnotta, M.; Murata, J.; Chen, J.; De Luca, V.
    Expression of deacetylvindoline-4-O-acetyltransferase in Catharanthus roseus hairy roots (2007), Phytochemistry, 68, 1922-1931.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.107 expressed in Escherichia coli and Catharanthus roseus Catharanthus roseus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.107 additional information DAT is overexpressed in Catharanthus roseus hairy root cultures: metabolite analysis using HPLC show that transgenic hairy root extracts have an altered alkaloid profile with respect to horhammericine accumulation. It is concluded that expression of DAT in hairy roots alters their monoterpenoid indole alkaloids profile. Evidence is provided that horhammericine accumulates via a DAT interaction with the root specific minovincinine-19-O-acetyltransferase (MAT) that inhibits the MAT mediated conversion of horhammericine into 19-O-acetyl-horhammericine Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.107 minovincinine-19-O-acetyltransferase incubation of DAT with varying amounts of crude recombinant minovincinine-19-O-acetyltransferase (MAT) inhibits DAT activity in a dose dependent manner Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.107 Catharanthus roseus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.107 hair root
-
 Catharanthus roseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.107 acetyl-CoA + deacetylvindoline
-
 Catharanthus roseus CoA + vindoline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.107 DAT
-
 Catharanthus roseus
2.3.1.107 deacetylvindoline-4-O-acetyltransferase
-
 Catharanthus roseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.107 37
-
 assay at Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.107 CoA
-
 Catharanthus roseus