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Literature summary extracted from

  • Holtgrefe, S.; Gohlke, J.; Starmann, J.; Druce, S.; Klocke, S.; Altmann, B.; Wojtera, J.; Lindermayr, C.; Scheibe, R.
    Regulation of plant cytosolic glyceraldehyde 3-phosphate dehydrogenase isoforms by thiol modifications (2008), Physiol. Plant., 133, 211-228.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.12 expressed in Escherichia coli BL21(DE3)pLysS cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.12 C159S the mutant C159S of the isozyme GapC2 shows decreased specific activity Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 glutathione inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol Arabidopsis thaliana
1.2.1.12 glutathione inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol Oryctolagus cuniculus
1.2.1.12 glutathione inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol Saccharomyces cerevisiae
1.2.1.12 glutathione inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol Spinacia oleracea
1.2.1.12 oxidized glutathione inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found glutathionylated; inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found glutathionylated Arabidopsis thaliana
1.2.1.12 oxidized glutathione inactivation, at least partially reversible upon addition of dithiothreitol Oryctolagus cuniculus
1.2.1.12 oxidized glutathione inactivation, at least partially reversible upon addition of dithiothreitol Spinacia oleracea
1.2.1.12 S-nitrosoglutathione inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found nitrosylated; inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found nitrosylated; inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol Arabidopsis thaliana
1.2.1.12 S-nitrosoglutathione inactivation, at least partially reversible upon addition of dithiothreitol; inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol Oryctolagus cuniculus
1.2.1.12 S-nitrosoglutathione inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol Saccharomyces cerevisiae
1.2.1.12 S-nitrosoglutathione inactivation, at least partially reversible upon addition of dithiothreitol; inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.12 cytosol
-
 Saccharomyces cerevisiae 5829
-
1.2.1.12 cytosol
-
 Oryctolagus cuniculus 5829
-
1.2.1.12 cytosol
-
 Spinacia oleracea 5829
-
1.2.1.12 cytosol
-
 Arabidopsis thaliana 5829
-
1.2.1.12 nucleus
-
 Arabidopsis thaliana 5634
-
1.2.1.12 nucleus of protoplast, both isoforms GapC1 and GapC2 Arabidopsis thaliana 5634
-
1.2.1.12 protoplast
-
 Arabidopsis thaliana
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Arabidopsis thaliana P25858 ecotype Columbia
-
1.2.1.12 Arabidopsis thaliana P25858 isoform GapC1
-
1.2.1.12 Arabidopsis thaliana Q56WJ4 isoform GapC2
-
1.2.1.12 Oryctolagus cuniculus
-
-
-
1.2.1.12 Saccharomyces cerevisiae
-
-
-
1.2.1.12 Spinacia oleracea
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.1.12 stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored Saccharomyces cerevisiae
1.2.1.12 stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored Oryctolagus cuniculus
1.2.1.12 stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored Spinacia oleracea
1.2.1.12 stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12 ammonium sulfate precipitation and Q-Sepharose column chromatography Saccharomyces cerevisiae
1.2.1.12 ammonium sulfate precipitation and Q-Sepharose column chromatography Oryctolagus cuniculus
1.2.1.12 ammonium sulfate precipitation and Q-Sepharose column chromatography Spinacia oleracea
1.2.1.12 ammonium sulfate precipitation and Q-Sepharose column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.12 flower
-
 Arabidopsis thaliana
-
1.2.1.12 leaf
-
 Spinacia oleracea
-
1.2.1.12 leaf
-
 Arabidopsis thaliana
-
1.2.1.12 muscle
-
 Oryctolagus cuniculus
-
1.2.1.12 silique
-
 Arabidopsis thaliana
-
1.2.1.12 stem
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 30
-
 purified isozyme GapC1 Arabidopsis thaliana
1.2.1.12 33
-
 purified GapC2 C159S mutant isozyme Arabidopsis thaliana
1.2.1.12 60
-
 purified isozyme GapC2 Arabidopsis thaliana
1.2.1.12 90
-
 purified enzyme Spinacia oleracea
1.2.1.12 100
-
 purified enzyme Saccharomyces cerevisiae
1.2.1.12 100
-
 purified enzyme Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Saccharomyces cerevisiae 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Oryctolagus cuniculus 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Spinacia oleracea 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Arabidopsis thaliana 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 More enzyme can bind top a partial gene sequence of NADP-dependent malated dehydrogenase EC 1.2.1.37 Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.2.1.12 cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase
-
 Saccharomyces cerevisiae
1.2.1.12 cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase
-
 Oryctolagus cuniculus
1.2.1.12 cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase
-
 Spinacia oleracea
1.2.1.12 cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase
-
 Arabidopsis thaliana
1.2.1.12 GapC1 isozyme Arabidopsis thaliana
1.2.1.12 GapC2 isozyme Arabidopsis thaliana
1.2.1.12 GAPDH
-
 Saccharomyces cerevisiae
1.2.1.12 GAPDH
-
 Oryctolagus cuniculus
1.2.1.12 GAPDH
-
 Spinacia oleracea
1.2.1.12 GAPDH
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Saccharomyces cerevisiae
1.2.1.12 NAD+
-
 Oryctolagus cuniculus
1.2.1.12 NAD+
-
 Spinacia oleracea
1.2.1.12 NAD+
-
 Arabidopsis thaliana