Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tu, S.C.
    Activity coupling and complex formation between bacterial luciferase and flavin reductases (2008), Photochem. Photobiol. Sci., 7, 183-188.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.3 additional information immobilization of the FMN reductase-luciferase complex Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.3 additional information
-
 additional information kinetics of FMN reductase-luciferase complex formation, overview Vibrio harveyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.30 24600
-
 2 * 24600, X-ray crystallography Aliivibrio fischeri
1.5.1.30 26300
-
 2 * 26300, X-ray crystallography Vibrio harveyi
1.5.1.30 49200
-
 X-ray crystallography Aliivibrio fischeri
1.5.1.30 52600
-
 X-ray crystallography Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.3 RCHO + FMNH2 + O2 Aliivibrio fischeri reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, NADPH-specific FMN reductase and luciferase form a complex in vivo, reduction of reductase-bound FMN cofactor by NADPH is reversible, allowing the cellular contents of NADP+ and NADPH as a factor for the regulation of the production of FMNH2 by FRPVh for luciferase bioluminescence, overview RCOOH + FMN + H2O + hnu
-
 ?
1.14.14.3 RCHO + FMNH2 + O2 Vibrio harveyi reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, NADPH-specific FMN reductase and luciferase form a complex in vivo, reduction of reductase-bound FMN cofactor by NADPH is reversible, allowing the cellular contents of NADP+ and NADPH as a factor for the regulation of the production of FMNH2 by FRPVh for luciferase bioluminescence, overview RCOOH + FMN + H2O + hnu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.30 Aliivibrio fischeri
-
-
-
1.5.1.30 Vibrio harveyi
-
-
-
1.14.14.3 Aliivibrio fischeri
-
-
-
1.14.14.3 Vibrio harveyi
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.30 reduced riboflavin + NADP+ = riboflavin + NADPH + H+ ping-pong mechanism Aliivibrio fischeri
a
1.5.1.30 reduced riboflavin + NADP+ = riboflavin + NADPH + H+ ping-pong mechanism Vibrio harveyi
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.30 FMN + NAD(P)H
-
 Aliivibrio fischeri FMNH2 + NAD(P)+
-
 r
1.5.1.30 FMN + NADPH
-
 Vibrio harveyi FMNH2 + NADP+
-
 r
1.5.1.30 riboflavin + NAD(P)H
-
 Aliivibrio fischeri reduced riboflavin + NAD(P)+
-
 r
1.5.1.30 riboflavin + NADPH
-
 Vibrio harveyi reduced riboflavin + NADP+
-
 r
1.14.14.3 RCHO + FMNH2 + O2
-
 Aliivibrio fischeri RCOOH + FMN + H2O + hnu
-
 ?
1.14.14.3 RCHO + FMNH2 + O2
-
 Vibrio harveyi RCOOH + FMN + H2O + hnu
-
 ?
1.14.14.3 RCHO + FMNH2 + O2 reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, NADPH-specific FMN reductase and luciferase form a complex in vivo, reduction of reductase-bound FMN cofactor by NADPH is reversible, allowing the cellular contents of NADP+ and NADPH as a factor for the regulation of the production of FMNH2 by FRPVh for luciferase bioluminescence, overview Aliivibrio fischeri RCOOH + FMN + H2O + hnu
-
 ?
1.14.14.3 RCHO + FMNH2 + O2 reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, NADPH-specific FMN reductase and luciferase form a complex in vivo, reduction of reductase-bound FMN cofactor by NADPH is reversible, allowing the cellular contents of NADP+ and NADPH as a factor for the regulation of the production of FMNH2 by FRPVh for luciferase bioluminescence, overview Vibrio harveyi RCOOH + FMN + H2O + hnu
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.30 homodimer 2 * 24600, X-ray crystallography Aliivibrio fischeri
1.5.1.30 homodimer 2 * 26300, X-ray crystallography Vibrio harveyi
1.5.1.30 monomer in solution FRPVh undergoes a monomer-dimer equilibrium Aliivibrio fischeri
1.5.1.30 monomer in solution FRPVh undergoes a monomer-dimer equilibrium Vibrio harveyi

Synonyms

EC Number Synonyms Comment Organism
1.5.1.30 FRGvf
-
 Aliivibrio fischeri
1.5.1.30 FRPvh
-
 Vibrio harveyi
1.14.14.3 luciferase
-
 Aliivibrio fischeri
1.14.14.3 luciferase
-
 Vibrio harveyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.30 FMN contains one FMN cofactor per monomer, the FMN cofactor of FRPVh can be replaced by 2-thioFMN to yield a catalytically active reconstituted reductase Aliivibrio fischeri
1.5.1.30 FMN contains one FMN cofactor per monomer, the FMN cofactor of FRPVh can be replaced by 2-thioFMN to yield a catalytically active reconstituted reductase Vibrio harveyi
1.5.1.30 NAD(P)H
-
 Aliivibrio fischeri
1.5.1.30 NADPH
-
 Vibrio harveyi
1.14.14.3 FMNH2 reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, overview Aliivibrio fischeri
1.14.14.3 FMNH2 reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, overview Vibrio harveyi