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Literature summary extracted from
- Homogeneous enzymatic reactions in ionic liquids with poly(ethylene glycol)-modified subtilisin (2006), Org. Biomol. Chem., 4, 3462-3467.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.62 | additional information | no activity of poly(ethylene glycol)-modified subtilisin in methyl tert-butyl ether, tetrahydrofuran, dimethylsulfoxide, and acetonitrile | Bacillus licheniformis | |
| 3.4.21.62 | poly(ethylene glycol) | poly(ethylene glycol)-modified subtilisin suspended in ionic liquids exhibits excellent catalytic performance while the native enzyme shows no activity | Bacillus licheniformis |  |
| 3.4.21.62 | Toluene | poly(ethylene glycol)-modified subtilisin | Bacillus licheniformis | |
| 3.4.21.62 | [C2OC1mim][Tf2N] | poly(ethylene glycol)-modified subtilisin | Bacillus licheniformis | |
| 3.4.21.62 | [C2OHmim][Tf2N] | poly(ethylene glycol)-modified subtilisin | Bacillus licheniformis | |
| 3.4.21.62 | [Emim][Tf2N] | poly(ethylene glycol)-modified subtilisin shows good stability in [Emim][Tf2N], and maintains 80% of its initial activity after 60 h. Preferred medium for enzymatic reaction. Enzyme activity is much higher than in conventional organic solvents. Excellent activity is associated with unique properties such as hydrophobicity and high polarity | Bacillus licheniformis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.62 | Bacillus licheniformis | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.62 | N-acetyl-L-phenylalanine ethyl ester + 1-butanol | transesterification catalyzed by poly(ethylene glycol)-modified subtilisin | Bacillus licheniformis | ? | - |
? | |
| 3.4.21.62 | p-nitrophenyl butyrate + H2O | catalyzed by native subtilisin | Bacillus licheniformis | p-nitrophenol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.62 | subtilisin Carlsberg | - |
Bacillus licheniformis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 8 | - |
- |
Bacillus licheniformis |
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