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Literature summary extracted from

  • Sergiev, P.V.; Bogdanov, A.A.; Dontsova, O.A.
    Ribosomal RNA guanine-(N2)-methyltransferases and their targets (2007), Nucleic Acids Res., 35, 2295-2301.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.171 S-adenosyl-L-methionine + guanine966 in 16S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + N2-methylguanine966 in 16S rRNA
-
 ?
2.1.1.172 S-adenosyl-L-methionine + guanine1207 in 16S rRNA Escherichia coli RsmD methyltransferase utilizes assembled ribosomal small subunit or its late assembly intermediates as a substrate. It is likely it uses the decoding cleft of the small subunit as the binding site. Nucleotide N2-methylguanine1207 is located in the helix 34 of the 16S rRNA. This functionally important helix is involved in the formation of the binding pocket for A-site bound tRNA and translocation. Despite location in the very functionally important region of the 30S subunit, N2-methylguanine1207 is not involved in the contact with tRNA. Methylation of G1207 may be necessary to freeze secondary structure in the functional conformation S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
 ?
2.1.1.173 S-adenosyl-L-methionine + guanine2445 in 23S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?
2.1.1.174 S-adenosyl-L-methionine + guanine1835 in 23S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.171 Escherichia coli P0ADX9
-
-
2.1.1.172 Escherichia coli P39406
-
-
2.1.1.173 Escherichia coli P75864
-
-
2.1.1.174 Escherichia coli Q0T0I4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.171 S-adenosyl-L-methionine + guanine966 in 16S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine966 in 16S rRNA
-
 ?
2.1.1.171 S-adenosyl-L-methionine + guanine966 in 16S rRNA RsmD methyltransferase utilizes assembled small subunits or its late assembly intermediates as a substrate. It is likely that the latter class of proteins uses the decoding cleft of the small subunit as the binding site Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine966 in 16S rRNA
-
 ?
2.1.1.172 S-adenosyl-L-methionine + guanine1207 in 16S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
 ?
2.1.1.172 S-adenosyl-L-methionine + guanine1207 in 16S rRNA RsmD methyltransferase utilizes assembled ribosomal small subunit or its late assembly intermediates as a substrate. It is likely it uses the decoding cleft of the small subunit as the binding site. Nucleotide N2-methylguanine1207 is located in the helix 34 of the 16S rRNA. This functionally important helix is involved in the formation of the binding pocket for A-site bound tRNA and translocation. Despite location in the very functionally important region of the 30S subunit, N2-methylguanine1207 is not involved in the contact with tRNA. Methylation of G1207 may be necessary to freeze secondary structure in the functional conformation Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
 ?
2.1.1.173 S-adenosyl-L-methionine + guanine2445 in 23S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?
2.1.1.173 S-adenosyl-L-methionine + guanine2445 in 23S rRNA RlmL possessing an additional RNA-binding domain acts on naked ribosomal RNA or early assembly intermediates in the cell Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?
2.1.1.174 S-adenosyl-L-methionine + guanine1835 in 23S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
 ?
2.1.1.174 S-adenosyl-L-methionine + guanine1835 in 23S rRNA RlmG possessing an additional RNA-binding domain act on naked ribosomal RNA or early assembly intermediates in the cell Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.171 RsmD
-
 Escherichia coli
2.1.1.171 yhhF
-
 Escherichia coli
2.1.1.172 RsmC
-
 Escherichia coli
2.1.1.172 yjjT
-
 Escherichia coli
2.1.1.173 RlmL
-
 Escherichia coli
2.1.1.173 ycbY
-
 Escherichia coli
2.1.1.174 ribosomal RNA large subunit methyltransferase G
-
 Escherichia coli
2.1.1.174 RlmG
-
 Escherichia coli
2.1.1.174 ygjO
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.1.1.173 malfunction lack of G2445 methylation as a consequence of deletion of the rlmL(ycbY) gene causes significant growth retardation Escherichia coli
2.1.1.174 malfunction lack of G1835 methylation in rlmG(ygjO) knockout strain does not lead to significant growth retardation at the optimal growth conditions. However, in the poor medium and at elevated temperature, the rlmG(ygjO) knockout strain has significantly decreased fitness Escherichia coli