Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, H.B.; Qin, Y.M.; Pang, Y.; Song, W.Q.; Mei, W.Q.; Zhu, Y.X.
    A cotton ascorbate peroxidase is involved in hydrogen peroxide homeostasis during fibre cell development (2007), New Phytol., 175, 462-471.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.11 GhAPX1 is expressed in Escherichia coli Gossypium hirsutum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.12
-
 H2O2
-
 Gossypium hirsutum
1.11.1.11 0.43
-
 L-ascorbate
-
 Gossypium hirsutum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 cytosol
-
 Gossypium hirsutum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.11 28000
-
 2 * 28000, calculated from sequence Gossypium hirsutum
1.11.1.11 31500
-
 2 * 31500, His-tag fused enzyme, SDS-PAGE Gossypium hirsutum
1.11.1.11 64200
-
 gel filtration Gossypium hirsutum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.11 additional information Gossypium hirsutum GhAPX1 is involved in hydrogen peroxide homeostasis during cotton fibre development ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Gossypium hirsutum A7KIX5 L. cv. Xuzhou 142
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.11
-
 Gossypium hirsutum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.11 cotton fibre GhAPX1 is highly expressed in wild-type 5-day postanthesis fibres with much lower transcript levels in the fuzzless-lintless mutant ovules. GhAPX1 expression is upregulated in response to an increase in cellular H2O2 and ethylene Gossypium hirsutum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 GSSG + H2O2 about 20% of the activity with L-ascorbate Gossypium hirsutum ?
-
 ?
1.11.1.11 guaiacol + H2O2 about 30% of the activity with L-ascorbate Gossypium hirsutum ?
-
 ?
1.11.1.11 L-ascorbate + H2O2
-
 Gossypium hirsutum dehydroascorbate + H2O
-
 ?
1.11.1.11 additional information GhAPX1 is involved in hydrogen peroxide homeostasis during cotton fibre development Gossypium hirsutum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.11 dimer 2 * 28000, calculated from sequence Gossypium hirsutum
1.11.1.11 dimer 2 * 31500, His-tag fused enzyme, SDS-PAGE Gossypium hirsutum

Synonyms

EC Number Synonyms Comment Organism
1.11.1.11 GhAPX1
-
 Gossypium hirsutum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.11 46.71
-
 H2O2
-
 Gossypium hirsutum
1.11.1.11 260
-
 L-ascorbate
-
 Gossypium hirsutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.11 6.5
-
 oxidation of ascorbate Gossypium hirsutum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.11 5 7.5 pH 5: about 50% of maximal activity, pH 7.5: about 50% of maximal activity Gossypium hirsutum