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Literature summary extracted from

  • Li, F.; Feng, Q.; Lee, C.; Wang, S.; Pelleymounter, L.L.; Moon, I.; Eckloff, B.W.; Wieben, E.D.; Schaid, D.J.; Yee, V.; Weinshilboum, R.M.
    Human betaine-homocysteine methyltransferase (BHMT) and BHMT2: common gene sequence variation and functional characterization (2008), Mol. Genet. Metab., 94, 326-335.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.5 expressed in COS-1 cells and in Escherichia coli Homo sapiens
2.1.1.5 impossible to express BHMT2 in mammalian cells, protein aggregates after bacterial expression. BHMT2 is rapidly degraded in a rabbit reticulocyte lysate, but it can be stabilized by cotransfection of COS-1 cells with BHMT and, after cotransfection, it coprecipitates with BHMT Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.5 A66V nonsynonymous SNP identified using 240 DNA samples from four ethnic groups Homo sapiens
2.1.1.5 Arg16Cys nonsynonymous SNP identified using 240 DNA samples from four ethnic groups, Km (mM): 0.0139 (betaine), 0.0076 (L-homocysteine) Homo sapiens
2.1.1.5 Arg239Gln nonsynonymous SNP identified using 240 DNA samples from four ethnic groups, Km (mM): 0.012 (betaine), 0.0158 (L-homocysteine) Homo sapiens
2.1.1.5 Gly199Ser nonsynonymous SNP identified using 240 DNA samples from four ethnic groups, Km (mM): 0.0139 (betaine), 0.0206 (L-homocysteine) Homo sapiens
2.1.1.5 Pro197Ser nonsynonymous SNP identified using 240 DNA samples from four ethnic groups, Km (mM): 0.0213 (betaine), 0.0216 (L-homocysteine) Homo sapiens
2.1.1.5 T218M nonsynonymous SNP identified using 240 DNA samples from four ethnic groups Homo sapiens
2.1.1.5 V155F nonsynonymous SNP identified using 240 DNA samples from four ethnic groups Homo sapiens
2.1.1.5 V237M nonsynonymous SNP identified using 240 DNA samples from four ethnic groups Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.5 0.0076
-
 L-homocysteine mutant Arg16Cys Homo sapiens
2.1.1.5 0.012
-
 betaine mutant Arg239Gln Homo sapiens
2.1.1.5 0.0139
-
 betaine mutant Arg16Cys Homo sapiens
2.1.1.5 0.0139
-
 betaine mutant Gly199Ser Homo sapiens
2.1.1.5 0.0158
-
 L-homocysteine mutant Arg239Gln Homo sapiens
2.1.1.5 0.0206
-
 L-homocysteine mutant Gly199Ser Homo sapiens
2.1.1.5 0.0213
-
 betaine mutant Pro197Ser Homo sapiens
2.1.1.5 0.0216
-
 L-homocysteine mutant Pro197Ser Homo sapiens
2.1.1.5 0.0227
-
 betaine wild-type Homo sapiens
2.1.1.5 0.029
-
 L-homocysteine wild-type Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.5 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.5 additional information no expression of BHMT2 protein is detected in liver or kidney although BHMT2 mRNA expression is observed Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.5 L-homocysteine + betaine
-
 Homo sapiens L-methionine + dimethylglycine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.5 betaine-homocysteine S-methyltransferase
-
 Homo sapiens
2.1.1.5 BHMT
-
 Homo sapiens
2.1.1.5 BHMT2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.5 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.5 8
-
 assay at Homo sapiens