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Literature summary extracted from

  • Ulusu, N.N.; Tandogan, B.
    Purification and kinetic properties of glutathione reductase from bovine liver (2007), Mol. Cell. Biochem., 303, 45-51.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 GSH With regard to GSSG as variable substrate at fixed NADPH concentration (0.1 mM), GSH is a non-competitive inhibitor. With regard to NADPH as variable substrate at fixed GSSG concentration, GSH is a non-competitive inhibitor Bos taurus
1.8.1.7 NADP+ With regard to GSSG as variable substrate at fixed NADPH concentration, NADP+ is an uncompetitive inhibitor. With regard to NADPH as variable substrate at fixed GSSG concentration, NADP+ is a competitive inhibitor Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.063
-
 NADPH pH 7.4, 37°C Bos taurus
1.8.1.7 0.154
-
 glutathione disulfide pH 7.4, 37°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 55000
-
 x * 55000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.7 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ the enzyme follows the Ping Pong mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 185.5
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH + H+
-
 Bos taurus glutathione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 ? x * 55000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 50
-
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 7
-
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 NADPH
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.7 0.043
-
 NADP+ pH 7.4, 37°C, NADPH as variable substrate at fixed GSSG concentration Bos taurus
1.8.1.7 0.219
-
 NADP+ pH 7.4, 37°C, GSSG as variable substrate at fixed NADPH concentration (0.1 mM) Bos taurus
1.8.1.7 7.985
-
 GSH pH 7.4, 37°C, GSSG as variable substrate at fixed NADPH concentration (0.1 mM) Bos taurus
1.8.1.7 8.506
-
 GSH pH 7.4, 37°C, NADPH as variable substrate at fixed GSSG concentration Bos taurus