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Literature summary extracted from

  • Sandefur, C.I.; Wooden, J.M.; Quaye, I.K.; Sirawaraporn, W.; Sibley, C.H.
    Pyrimethamine-resistant dihydrofolate reductase enzymes of Plasmodium falciparum are not enzymatically compromised in vitro (2007), Mol. Biochem. Parasitol., 154, 1-5.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.3 additional information pyrimethamine-resistant quadruple mutant 51I/59R/108N/213A, kcat value 0.68 per s, Km value for dihydrofolate, 0.0102 mM, Km value for NADPH, 0.0309 mM Plasmodium falciparum
1.5.1.3 N51I/C59R/S108N pyrimethamine-resistant mutant, 50% decrease in Km value Plasmodium falciparum
1.5.1.3 N51I/C59R/S108N/I164L pyrimethamine-resistant mutant, 50% decrease in Km value Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.0207
-
 7,8-dihydrofolate mutant N51I/C59R/S108N/I164L , pH 8.0, 25°C Plasmodium falciparum
1.5.1.3 0.0209
-
 7,8-dihydrofolate mutant N51I/C59R/S108N, pH 8.0, 25°C Plasmodium falciparum
1.5.1.3 0.0232
-
 NADPH wild-type, pH 8.0, 25°C Plasmodium falciparum
1.5.1.3 0.0373
-
 NADPH mutant N51I/C59R/S108N/I164L , pH 8.0, 25°C Plasmodium falciparum
1.5.1.3 0.0379
-
 NADPH mutant N51I/C59R/S108N, pH 8.0, 25°C Plasmodium falciparum
1.5.1.3 0.0444
-
 7,8-dihydrofolate wild-type, pH 8.0, 25°C Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Plasmodium falciparum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
 Plasmodium falciparum 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 1.23
-
 7,8-dihydrofolate mutant N51I/C59R/S108N, pH 8.0, 25°C Plasmodium falciparum
1.5.1.3 1.24
-
 7,8-dihydrofolate wild-type, pH 8.0, 25°C Plasmodium falciparum
1.5.1.3 1.42
-
 7,8-dihydrofolate mutant N51I/C59R/S108N/I164L , pH 8.0, 25°C Plasmodium falciparum