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Literature summary extracted from

  • Kudo, H.; Goto, T.; Saito, T.; Saito, H.; Otsuka, K.; Mitsubayashi, K.
    Biochemical sniffer with choline oxidase for measurement of choline vapour (2008), Microchim. Acta, 160, 421-426.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.3.17 biotechnology development and application of enzyme-based gas sensor Alcaligenes sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.17 choline + O2 Alcaligenes sp.
-
 betaine aldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.17 Alcaligenes sp. P16101
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.17 commercial preparation
-
 Alcaligenes sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.17 additional information
-
 sensor consisting of Clark-type dissolved oxygen electrode and choline oxidase immobilized membrane, choline concentration measured by the oxygen consumption induced by an enzyme reaction of choline oxidase, calibration curves for choline in the liquid and gas phases, response time for choline vapour 15% slower than that of biosensor for choline solution Alcaligenes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.17 choline + O2
-
 Alcaligenes sp. betaine aldehyde + H2O2
-
 ?
1.1.3.17 choline + O2 enzyme-based gas sensor, responses and calibration properties of the sensor for both of liquid and gaseous phases, various choline concentrations Alcaligenes sp. betaine aldehyde + H2O2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.17 8
-
 choline determination in the liquid phase at Alcaligenes sp.