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Literature summary extracted from

  • Zhang, L.; Shoun, H.
    Purification and functional analysis of fungal nitric oxide reductase cytochrome P450nor (2008), Methods Enzymol., 437, 117-133.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.14 expressed in Escherichia coli JM109(DE3) cells Cutaneotrichosporon cutaneum
1.7.2.5
-
 Aspergillus oryzae
1.7.2.5
-
 Fusarium lichenicola
1.7.2.5 expression in Escherichia coli Cutaneotrichosporon cutaneum
1.7.2.5 expression in Escherichia coli Fusarium oxysporum

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.14 S73G/S75GS75G the mutant exhibits 79% activity of wild type with NADH Fusarium oxysporum
1.7.1.14 S75G the mutant exhibits 63% activity of wild type with NADH Fusarium oxysporum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.1.14 mitochondrion
-
 Cutaneotrichosporon cutaneum 5739
-
1.7.1.14 mitochondrion
-
 Aspergillus oryzae 5739
-
1.7.1.14 mitochondrion
-
 Fusarium oxysporum 5739
-
1.7.2.5 mitochondrion
-
 Cutaneotrichosporon cutaneum 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.14 45200
-
 calculated from amino acid sequence Cutaneotrichosporon cutaneum
1.7.2.5 43000
-
 x * 43000, SDS-PAGE Cutaneotrichosporon cutaneum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.5 nitric oxide + NAD(P)H + H+ Cutaneotrichosporon cutaneum cytochrome P450nor is a nitric oxide reductase involved in fungal denitrification nitrous oxide + NAD(P)+ + H2O
-
 ?
1.7.2.5 nitric oxide + NADH + H+ Fusarium oxysporum cytochrome P450nor is a nitric oxide reductase involved in fungal denitrification nitrous oxide + NAD+
-
 ?
1.7.2.5 nitric oxide + NADH + H+ Aspergillus oryzae cytochrome P450nor is a nitric oxide reductase involved in fungal denitrification nitrous oxide + NAD+ + H2O
-
 ?
1.7.2.5 nitric oxide + NADH + H+ Fusarium lichenicola cytochrome P450nor is a nitric oxide reductase involved in fungal denitrification nitrous oxide + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.14 Aspergillus oryzae
-
-
-
1.7.1.14 Cutaneotrichosporon cutaneum
-
-
-
1.7.1.14 Fusarium lichenicola
-
-
-
1.7.1.14 Fusarium oxysporum
-
-
-
1.7.2.5 Aspergillus oryzae Q8NKB4
-
-
1.7.2.5 Cutaneotrichosporon cutaneum Q96WS9
-
-
1.7.2.5 Fusarium lichenicola Q00616
-
-
1.7.2.5 Fusarium lichenicola Q12599
-
-
1.7.2.5 Fusarium oxysporum P23295
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.14 DEAE cellulose column chromatography, phenyl-Superose column chromatography, and Mono Q column chromatography or Resource Q column chromatography Cutaneotrichosporon cutaneum
1.7.2.5
-
 Aspergillus oryzae
1.7.2.5
-
 Cutaneotrichosporon cutaneum
1.7.2.5
-
 Fusarium oxysporum
1.7.2.5
-
 Fusarium lichenicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.14 additional information P450nor is a reductase without monooxygenase activity Cutaneotrichosporon cutaneum ?
-
 ?
1.7.1.14 additional information P450nor is a reductase without monooxygenase activity Aspergillus oryzae ?
-
 ?
1.7.1.14 additional information P450nor is a reductase without monooxygenase activity Fusarium oxysporum ?
-
 ?
1.7.1.14 additional information P450nor is a reductase without monooxygenase activity Fusarium lichenicola ?
-
 ?
1.7.1.14 NO + NADH + H+
-
 Cutaneotrichosporon cutaneum N2O + NAD+ + H2O
-
 ?
1.7.1.14 NO + NADH + H+
-
 Aspergillus oryzae N2O + NAD+ + H2O
-
 ?
1.7.1.14 NO + NADH + H+
-
 Fusarium lichenicola N2O + NAD+ + H2O
-
 ?
1.7.1.14 NO + NADH + H+ Fnor is specific for NADH Fusarium oxysporum N2O + NAD+ + H2O
-
 ?
1.7.1.14 NO + NADPH + H+
-
 Cutaneotrichosporon cutaneum N2O + NADP+ + H2O
-
 ?
1.7.1.14 NO + NADPH + H+
-
 Aspergillus oryzae N2O + NADP+ + H2O
-
 ?
1.7.1.14 NO + NADPH + H+
-
 Fusarium lichenicola N2O + NADP+ + H2O
-
 ?
1.7.2.5 nitric oxide + NAD(P)H + H+ cytochrome P450nor is a nitric oxide reductase involved in fungal denitrification Cutaneotrichosporon cutaneum nitrous oxide + NAD(P)+ + H2O
-
 ?
1.7.2.5 nitric oxide + NADH + H+ cytochrome P450nor is a nitric oxide reductase involved in fungal denitrification Fusarium oxysporum nitrous oxide + NAD+
-
 ?
1.7.2.5 nitric oxide + NADH + H+ cytochrome P450nor is a nitric oxide reductase involved in fungal denitrification Aspergillus oryzae nitrous oxide + NAD+ + H2O
-
 ?
1.7.2.5 nitric oxide + NADH + H+ cytochrome P450nor is a nitric oxide reductase involved in fungal denitrification Fusarium lichenicola nitrous oxide + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.5 ? x * 43000, SDS-PAGE Cutaneotrichosporon cutaneum

Synonyms

EC Number Synonyms Comment Organism
1.7.1.14 Anor
-
 Aspergillus oryzae
1.7.1.14 Cnor1 isozyme Fusarium lichenicola
1.7.1.14 Cnor2 isozyme Fusarium lichenicola
1.7.1.14 CYP55A1
-
 Fusarium oxysporum
1.7.1.14 CYP55A2
-
 Fusarium lichenicola
1.7.1.14 CYP55A3
-
 Fusarium lichenicola
1.7.1.14 CYP55A4
-
 Cutaneotrichosporon cutaneum
1.7.1.14 CYP55A5
-
 Aspergillus oryzae
1.7.1.14 cytochrome P450nor
-
 Cutaneotrichosporon cutaneum
1.7.1.14 cytochrome P450nor
-
 Aspergillus oryzae
1.7.1.14 cytochrome P450nor
-
 Fusarium oxysporum
1.7.1.14 cytochrome P450nor
-
 Fusarium lichenicola
1.7.1.14 Fnor
-
 Fusarium oxysporum
1.7.1.14 fungal nitric oxide reductase
-
 Cutaneotrichosporon cutaneum
1.7.1.14 fungal nitric oxide reductase
-
 Aspergillus oryzae
1.7.1.14 fungal nitric oxide reductase
-
 Fusarium oxysporum
1.7.1.14 fungal nitric oxide reductase
-
 Fusarium lichenicola
1.7.1.14 P450nor
-
 Cutaneotrichosporon cutaneum
1.7.1.14 P450nor
-
 Aspergillus oryzae
1.7.1.14 P450nor
-
 Fusarium oxysporum
1.7.1.14 P450nor
-
 Fusarium lichenicola
1.7.1.14 Tnor
-
 Cutaneotrichosporon cutaneum
1.7.2.5 Anor
-
 Aspergillus oryzae
1.7.2.5 Cnor1
-
 Fusarium lichenicola
1.7.2.5 Cnor2
-
 Fusarium lichenicola
1.7.2.5 Fnor
-
 Fusarium oxysporum
1.7.2.5 nitric oxide reductase cytochrome
-
 Aspergillus oryzae
1.7.2.5 nitric oxide reductase cytochrome
-
 Cutaneotrichosporon cutaneum
1.7.2.5 nitric oxide reductase cytochrome
-
 Fusarium oxysporum
1.7.2.5 nitric oxide reductase cytochrome
-
 Fusarium lichenicola
1.7.2.5 P450nor
-
 Aspergillus oryzae
1.7.2.5 P450nor
-
 Cutaneotrichosporon cutaneum
1.7.2.5 P450nor
-
 Fusarium oxysporum
1.7.2.5 P450nor
-
 Fusarium lichenicola
1.7.2.5 Tnor
-
 Cutaneotrichosporon cutaneum

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.14 heme heme-thiolate protein Cutaneotrichosporon cutaneum
1.7.1.14 heme heme-thiolate protein Aspergillus oryzae
1.7.1.14 heme heme-thiolate protein Fusarium oxysporum
1.7.1.14 heme heme-thiolate protein Fusarium lichenicola
1.7.1.14 NADH Anor can utilize both NADH and NADPH Aspergillus oryzae
1.7.1.14 NADH Fnor is specific for NADH Fusarium oxysporum
1.7.1.14 NADH isozyme Cnor1 is specific for NADH Fusarium lichenicola
1.7.1.14 NADH Tnor can utilize both NADH and NADPH Cutaneotrichosporon cutaneum
1.7.1.14 NADPH Anor can utilize both NADH and NADPH Aspergillus oryzae
1.7.1.14 NADPH isozyme Cnor2 can utilize both NADH and NADPH Fusarium lichenicola
1.7.1.14 NADPH Nor activity of wild type with NADPH as electron donor is only 13% of that with NADH as electron donor Fusarium oxysporum
1.7.1.14 NADPH Tnor can utilize both NADH and NADPH Cutaneotrichosporon cutaneum
1.7.2.5 NADH
-
 Aspergillus oryzae
1.7.2.5 NADH
-
 Cutaneotrichosporon cutaneum
1.7.2.5 NADH
-
 Fusarium oxysporum
1.7.2.5 NADH
-
 Fusarium lichenicola
1.7.2.5 NADPH
-
 Cutaneotrichosporon cutaneum

General Information

EC Number General Information Comment Organism
1.7.1.14 physiological function P450nor is involved in fungal denitrification, it receives electrons directly from the distal pocket from NADH and/or NADPH without any help from a flavoprotein Cutaneotrichosporon cutaneum
1.7.1.14 physiological function P450nor is involved in fungal denitrification, it receives electrons directly from the distal pocket from NADH and/or NADPH without any help from a flavoprotein Aspergillus oryzae
1.7.1.14 physiological function P450nor is involved in fungal denitrification, it receives electrons directly from the distal pocket from NADH and/or NADPH without any help from a flavoprotein Fusarium oxysporum
1.7.1.14 physiological function P450nor is involved in fungal denitrification, it receives electrons directly from the distal pocket from NADH and/or NADPH without any help from a flavoprotein Fusarium lichenicola