EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.B4 | 0.075 | - |
2-propanol | - |
Pseudomonas sp. | |
1.1.1.B4 | 0.525 | - |
NAD+ | - |
Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.B4 | Pseudomonas sp. | - |
growth on 1-phenyl-1,2-ethanediol as sole carbon source | - |
1.1.1.B4 | Pseudomonas sp. PED | - |
growth on 1-phenyl-1,2-ethanediol as sole carbon source | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.B4 | (R)-R-CHOH-R' + NAD+ = R-CO-R' + NADH + H+ | kinetic mechanism is ordered bi-bi with the cofactor binding first and releasing last. The enzyme transfers the pro-R hydride of NADH to the si face of carbonyl compounds to yield (R)-alcohols | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.B4 | 36 | - |
pH 8.5, 30°C, substrate 2-propanol | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.B4 | 1-(2-phenylcyclopropyl)ethanone + NADH | - |
Pseudomonas sp. | (1R)-1-(2-phenylcyclopropyl)ethanol + NAD+ | 18% of the activity with 1-phenylpropane-1,2-dione | r | |
1.1.1.B4 | 1-(2-phenylcyclopropyl)ethanone + NADH | - |
Pseudomonas sp. PED | (1R)-1-(2-phenylcyclopropyl)ethanol + NAD+ | 18% of the activity with 1-phenylpropane-1,2-dione | r | |
1.1.1.B4 | 1-chloropentane-2,4-dione + NADH | - |
Pseudomonas sp. | (4R)-5-chloro-4-hydroxypentan-2-one + NAD+ | 76% yield, 98% enantiomeric excess | ? | |
1.1.1.B4 | 1-chloropentane-2,4-dione + NADH | - |
Pseudomonas sp. PED | (4R)-5-chloro-4-hydroxypentan-2-one + NAD+ | 76% yield, 98% enantiomeric excess | ? | |
1.1.1.B4 | 1-phenoxypropan-2-one + NADH | - |
Pseudomonas sp. | (2R)-1-phenoxypropan-2-ol + NAD+ | 21% of the activity with 1-phenylpropane-1,2-dione | r | |
1.1.1.B4 | 1-phenoxypropan-2-one + NADH | - |
Pseudomonas sp. PED | (2R)-1-phenoxypropan-2-ol + NAD+ | 21% of the activity with 1-phenylpropane-1,2-dione | r | |
1.1.1.B4 | 1-phenylethanone + NADH | - |
Pseudomonas sp. | (1R)-1-phenylethanol + NAD+ | 34% yield, 94% enantiomeric excess | ? | |
1.1.1.B4 | 1-phenylethanone + NADH | - |
Pseudomonas sp. PED | (1R)-1-phenylethanol + NAD+ | 34% yield, 94% enantiomeric excess | ? | |
1.1.1.B4 | 1-phenylpropane-1,2-dione + NADH | - |
Pseudomonas sp. | (1S)-1-hydroxy-1-phenylpropan-2-one + NAD+ | 83% yield, 86% enantiomeric excess | ? | |
1.1.1.B4 | 1-phenylpropane-1,2-dione + NADH | - |
Pseudomonas sp. PED | (1S)-1-hydroxy-1-phenylpropan-2-one + NAD+ | 83% yield, 86% enantiomeric excess | ? | |
1.1.1.B4 | 1-phenylpropane-1,2-dione + NADH | - |
Pseudomonas sp. | ? + NAD+ | - |
r | |
1.1.1.B4 | 2,2,2-trifluoro-1-phenylethanone + NADH + H+ | - |
Pseudomonas sp. | (1R)-2,2,2-trifluoro-1-phenylethanol + NAD+ | 21% of the activity with 1-phenylpropane-1,2-dione | r | |
1.1.1.B4 | 2,2,2-trifluoro-1-phenylethanone + NADH + H+ | - |
Pseudomonas sp. | (1S)-2,2,2-trifluoro-1-phenylethanol + NAD+ | 37% yield, 92% enantiomeric excess | ? | |
1.1.1.B4 | 2-butanone + NADH | - |
Pseudomonas sp. | (S)-2-hydroxybutane + NAD+ | - |
r | |
1.1.1.B4 | 2-pentanone + NADH + H+ | 6% of the activity with 2-butanone | Pseudomonas sp. | (S)-2-hydroxypentane + NAD+ | - |
r | |
1.1.1.B4 | 2-propanol + NAD+ + H+ | - |
Pseudomonas sp. | acetone + NADH | - |
r | |
1.1.1.B4 | 3-(dimethylamino)-1-phenylpropan-1-one + NADH | - |
Pseudomonas sp. | (1S)-3-dimethylamino-1-phenylpropan-1-ol + NAD+ | 24% of the activity with 1-phenylpropane-1,2-dione | r | |
1.1.1.B4 | 3-chloro-pentane-2,4-dione + NADH | 336% of the activity with 2-butanone | Pseudomonas sp. | (4R)-3-chloro-4-hydroxypentan-2-one + NAD+ | - |
r | |
1.1.1.B4 | 4-phenylbutan-2-one + NADH | - |
Pseudomonas sp. | (2R)-4-phenylbutan-2-ol + NAD+ | 18% of the activity with 1-phenylpropane-1,2-dione | r | |
1.1.1.B4 | 5-chloropentan-2-one + NADH | - |
Pseudomonas sp. | (2S)-5-chloropentan-2-ol + NAD+ | 48% yield, 93% enantiomeric excess | ? | |
1.1.1.B4 | 6-methylhept-5-en-2-one + NADH | - |
Pseudomonas sp. | (2S)-6-methylhept-5-en-2-ol + NAD+ | 51% yield, 97% enantiomeric excess | ? | |
1.1.1.B4 | methyl 3-oxobutanoate + NADH | 135% of the activity with 2-butanone | Pseudomonas sp. | methyl (3S)-3-hydroxybutanoate + NAD+ | - |
r | |
1.1.1.B4 | methyl oxo(phenyl)acetate + NADH | - |
Pseudomonas sp. | methyl (2S)-hydroxy(phenyl)ethanoate + NAD+ | 79% yield, 98% enantiomeric excess | ? | |
1.1.1.B4 | pentane-2,4-dione + NADH | 32% of the activity with 2-butanone | Pseudomonas sp. | (4R)-4-hydroxypentan-2-one + NAD+ | - |
r |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.B4 | 7.1 | - |
reduction | Pseudomonas sp. |
1.1.1.B4 | 8.5 | - |
oxidation | Pseudomonas sp. |