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Literature summary extracted from

  • Kizaki, N.; Yasohara, Y.; Nagashima, N.; Hasegawa, J.
    Characterization of novel alcohol dehydrogenase of Devosia riboflavina involved in stereoselective reduction of 3-pyrrolidinone derivatives (2008), J. Mol. Catal. B, 51, 73-80.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.1 iodoacetate 1 mM, 1.1fold activation Devosia riboflavina

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 1,10-phenanthroline 1 mM, 6% inhibition Devosia riboflavina
1.1.1.1 2,2'-bipyridyl 1 mM, 11% inhibition Devosia riboflavina
1.1.1.1 2,4-dinitrophenol 1 mM, complete inhibition Devosia riboflavina
1.1.1.1 2-mercaptoethanol 1 mM, 19% inhibition Devosia riboflavina
1.1.1.1 AgNO3 1 mM, 37% inhibition Devosia riboflavina
1.1.1.1 BaCl2 1 mM, complete inhibition Devosia riboflavina
1.1.1.1 dithiothreitol 1 mM, 6% inhibition Devosia riboflavina
1.1.1.1 EDTA 1 mM, 31% inhibition Devosia riboflavina
1.1.1.1 FeSO4 1 mM, complete inhibition Devosia riboflavina
1.1.1.1 HgCl2 1 mM, complete inhibition Devosia riboflavina
1.1.1.1 N-ethylmaleimide 1 mM, 11% inhibition Devosia riboflavina
1.1.1.1 PbCl2 1 mM, complete inhibition Devosia riboflavina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.58
-
 N-benzyl-3-pyrrolidinone
-
 Devosia riboflavina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Cu2+ 1 mM CuSO4, 1.7fold activation Devosia riboflavina
1.1.1.1 Li+ 1 mM LiCl, 1.1fold activation Devosia riboflavina
1.1.1.1 Ni2+ 1 mM NiSO4, 1.2fold activation Devosia riboflavina
1.1.1.1 Zn2+ 1 mM ZnSO4, 1.3fold activation Devosia riboflavina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 28000
-
 2 * 28000, SDS-PAGE Devosia riboflavina
1.1.1.1 58000
-
 gel filtration Devosia riboflavina

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Devosia riboflavina
-
 KNK10702
-
1.1.1.1 Devosia riboflavina KNK10702
-
 KNK10702
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Devosia riboflavina

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 3.12
-
-
 Devosia riboflavina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 (R)-2-butanol + NAD+ 55% of activity with N-benzyl-3-pyrrolidinol Devosia riboflavina 2-butanone + NADH + H+
-
 ?
1.1.1.1 (R)-2-butanol + NAD+ 55% of activity with N-benzyl-3-pyrrolidinol Devosia riboflavina KNK10702 2-butanone + NADH + H+
-
 ?
1.1.1.1 1-octanol + NAD+ 6% of activity with N-benzyl-3-pyrrolidinol Devosia riboflavina 1-octanal + NADH + H+
-
 ?
1.1.1.1 1-phenyl-2-butanone + NADH + H+ 1.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-phenylbutan-2-ol + NAD+
-
 ?
1.1.1.1 1-phenyl-2-butanone + NADH + H+ 1.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina KNK10702 1-phenylbutan-2-ol + NAD+
-
 ?
1.1.1.1 2-acetylpyrrole + NADH + H+ 70% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-(1H-pyrrol-2-yl)ethanol + NAD+
-
 ?
1.1.1.1 2-butanone + NADH + H+ 2.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina butan-2-ol + NAD+
-
 ?
1.1.1.1 2-hexanone + NADH + H+ 50.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina hexan-2-ol + NAD+
-
 ?
1.1.1.1 2-nitrobenzaldehyde + NADH + H+ 33% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina (2-nitrophenyl)methanol + NAD+
-
 ?
1.1.1.1 2-oxo-butyric acid + NADH + H+ 15% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 2-hydroxybutyric acid + NAD+
-
 ?
1.1.1.1 2-pentanol + NAD+ 45% of activity with N-benzyl-3-pyrrolidinol Devosia riboflavina 2-pentanone + NADH + H+
-
 ?
1.1.1.1 2-pentanol + NAD+ 45% of activity with N-benzyl-3-pyrrolidinol Devosia riboflavina KNK10702 2-pentanone + NADH + H+
-
 ?
1.1.1.1 3-acetylpyridine + NADH + H+ 7.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-pyridin-3-ylethanol + NAD+
-
 ?
1.1.1.1 3-phenylpropionaldehyde + NADH + H+ 6.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 3-phenylpropanol + NAD+
-
 ?
1.1.1.1 4-acetylpyridine + NADH + H+ 64.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-pyridin-4-ylethanol + NAD+
-
 ?
1.1.1.1 acetone + NADH + H+ 1.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina propan-2-ol + NAD+
-
 ?
1.1.1.1 acetophenone + NADH + H+ 1.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-phenylethanol + NAD+
-
 ?
1.1.1.1 benzaldehyde + NADH + H+ 21% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina benzyl alcohol + NAD+
-
 ?
1.1.1.1 benzoin + NADH + H+ 13% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1,2-diphenylethane-1,2-diol
-
 ?
1.1.1.1 benzyloxycarbonyl-3-pyrrolidinone + NADH + H+ production with 99.6% enantiomeric excess Devosia riboflavina (S)-benzyloxycarbonyl-3-pyrrolidinol + NAD+
-
 ?
1.1.1.1 chloroacetone + NADH + H+ 41.7fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-chloropropan-2-ol + NAD+
-
 ?
1.1.1.1 diacetyl + NADH + H+ 16.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
1.1.1.1 diacetyl + NADH + H+ 16.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina KNK10702 ?
-
 ?
1.1.1.1 dihydro-4,4-dimethyl-2,3-furandione + NADH + H+ 13.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
1.1.1.1 ethyl 4-chloroacetoacetate + NADH + H+ 6.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
1.1.1.1 ethyl acetoacetate + NADH + H+ 42.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
1.1.1.1 glutaraldehyde + NADH + H+ 70.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
1.1.1.1 hexanal + NADH + H+ 22.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina n-hexanol + NAD+
-
 ?
1.1.1.1 levulinic acid + NADH + H+ 11% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
1.1.1.1 m-chlorophenacyl chloride + NADH + H+ 4.6fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
 ?
1.1.1.1 methyl glyoxal + NADH + H+ 3.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
1.1.1.1 methyl pyruvate + NADH + H+ 18.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina methyl 2-hydroxypropanoate + NAD+
-
 ?
1.1.1.1 additional information when NADH is replaced with NADPH, the reaction rate is reduced by 0.6% Devosia riboflavina ?
-
 ?
1.1.1.1 additional information when NADH is replaced with NADPH, the reaction rate is reduced by 0.6% Devosia riboflavina KNK10702 ?
-
 ?
1.1.1.1 N-benzyl-3-piperidone + NADH + H+ 2.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina N-benzylpiperidin-3-ol + NAD+
-
 ?
1.1.1.1 N-benzyl-3-pyrrolidinone + NADH + H+ (R)-stereoselectivity of the reduction carried out with the heat-treated cells Devosia riboflavina (R)-N-benzyl-3-pyrrolidinol + NAD+
-
 r
1.1.1.1 N-benzyl-3-pyrrolidinone + NADH + H+
-
 Devosia riboflavina (S)-N-benzyl-3-pyrrolidinol + NAD+
-
 r
1.1.1.1 N-benzyl-4-piperidone + NADH + H+ 10.7fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina N-benzylpiperidin-4-ol + NAD+
-
 ?
1.1.1.1 n-butyraldehyde + NADH + H+ 7.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina n-butanol + NAD+
-
 ?
1.1.1.1 N-tert-butoxycarbonyl-3-pyrrolidinone + NADH + H+ production of (S)-N-tert-butoxycarbonyl-3-pyrrolidinol with 99.6% enantiomeric excess Devosia riboflavina (S)-N-tert-butoxycarbonyl-3-pyrrolidinol + NAD+
-
 ?
1.1.1.1 oxalacetic acid + NADH + H+ 14% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina ?
-
 ?
1.1.1.1 p-chlorobenzaldehyde + NADH + H+ 1.2fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina p-chlorobenzyl alcohol + NAD+
-
 ?
1.1.1.1 p-nitrobenzyloxycarbonyl-3-pyrrolidinone + NADH + H+ production with 90.7% enantiomeric excess Devosia riboflavina (S)-N-p-nitrobenzyloxycarbonyl-3-pyrrolidinol + NAD+
-
 ?
1.1.1.1 phenylethanol + NAD+ 15% of activity with N-benzyl-3-pyrrolidinol Devosia riboflavina phenylacetaldehyde + NADH + H+
-
 ?
1.1.1.1 propiophenone + NADH + H+ 27% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina 1-phenylpropan-1-ol + NAD+
-
 ?
1.1.1.1 pyridine-3-aldehyde + NADH + H+ 82% of activity with N-benzyl-3-pyrrolidinone Devosia riboflavina pyridin-3-yl ethanol + NAD+
-
 ?
1.1.1.1 pyridine-4-aldehyde + NADH + H+ 4.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone Devosia riboflavina pyridin-4-yl methanol + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 28000, SDS-PAGE Devosia riboflavina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 55
-
-
 Devosia riboflavina

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1 30 65 30°C: 36% of maximal activity, 65°C: 24% of maximal activity Devosia riboflavina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 30
-
 1 h, 90 mM potassium phosphate buffer, pH 6.5, stable Devosia riboflavina
1.1.1.1 45
-
 1 h, 90 mM potassium phosphate buffer, pH 6.5, 70% loss of activity Devosia riboflavina
1.1.1.1 50
-
 1 h, 90 mM potassium phosphate buffer, pH 6.5, complete loss of activity Devosia riboflavina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 5.7
-
-
 Devosia riboflavina