EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.5.99.12 | hanging-drop vapor-diffusion method in the presence of N6-(buta-2,3-dienyl)adenine or N6-(penta-2,3-dienyl)adenine | Zea mays |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.12 | N6-(2-bromo-2-isopentenyl)adenine | weak inhibition | Zea mays | |
1.5.99.12 | N6-(2-butynyl)adenine | irreversible inhibition | Zea mays | |
1.5.99.12 | N6-(4-hydroxy-2-butynyl)adenine | irreversible inhibition | Zea mays | |
1.5.99.12 | N6-(buta-2,3-dienyl)adenine | strong irreversible mechanism based inhibition | Zea mays | |
1.5.99.12 | N6-(penta-2,3-dienyl)adenine | strong irreversible mechanism based inhibition | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.99.12 | 0.0011 | - |
N6-(2-isopentenyl)adenine | 37°C | Zea mays | |
1.5.99.12 | 0.092 | - |
N6-(2-butynyl)adenine | 37°C | Zea mays | |
1.5.99.12 | 0.38 | - |
N6-(4-hydroxy-2-butynyl)adenine | 37°C | Zea mays |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.99.12 | 69865 | - |
1 * 69865, MALDI-TOF MS, crystal structure analysis | Zea mays |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.12 | additional information | Zea mays | irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde, important for the regulation of early development of the plant embryo | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.99.12 | Zea mays | Q9T0N8 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.5.99.12 | glycoprotein | - |
Zea mays |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.99.12 | embryo | strong expression in kernel | Zea mays | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.12 | additional information | irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde, important for the regulation of early development of the plant embryo | Zea mays | ? | - |
? | |
1.5.99.12 | N6-(2-butynyl)adenine + 2,6-dichlorophenol indophenol | very effective electron acceptor | Zea mays | ? | - |
? | |
1.5.99.12 | N6-(2-isopentenyl)adenine + 2,6-dichlorophenol indophenol | very effective electron acceptor | Zea mays | ? | - |
? | |
1.5.99.12 | N6-(2-isopentenyl)adenosine + 2,6-dichlorophenol indophenol | very effective electron acceptor | Zea mays | ? | - |
? | |
1.5.99.12 | N6-(4-hydroxy-2-butynyl)adenine + 2,6-dichlorophenol indophenol | very effective electron acceptor | Zea mays | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.99.12 | monomer | 1 * 69865, MALDI-TOF MS, crystal structure analysis | Zea mays |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.99.12 | CKX | - |
Zea mays |
1.5.99.12 | cytokinin oxidase/dehydrogenase | - |
Zea mays |
1.5.99.12 | ZmCKO1 | - |
Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.12 | FAD | - |
Zea mays |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.99.12 | 0.01 | - |
N6-(buta-2,3-dienyl)adenine | - |
Zea mays | |
1.5.99.12 | 0.026 | - |
N6-(penta-2,3-dienyl)adenine | - |
Zea mays |