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Literature summary extracted from

  • Agren, D.; Stehr, M.; Berthold, C.L.; Kapoor, S.; Oehlmann, W.; Singh, M.; Schneider, G.
    Three-dimensional structures of apo- and holo-L-alanine dehydrogenase from Mycobacterium tuberculosis reveal conformational changes upon coenzyme binding (2008), J. Mol. Biol., 377, 1161-1173.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.1 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.1 hanging drop vapour diffusion method, crystals of apo-AlaDH are grown using 0.4 M ammonium hydrogenphosphate as reservoir solution at 20°C Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.1 D270A inactive Mycobacterium tuberculosis
1.4.1.1 D270N inactive, the bifurcated hydrogen bond between Asp270 and the ribose of NAD is replaced by a single hydrogen bond Mycobacterium tuberculosis
1.4.1.1 H96A inactive Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.76
-
pyruvate wild type enzyme, in 1 M NH4Cl/NH3, pH 7.4, at 25°C Mycobacterium tuberculosis
1.4.1.1 15.64
-
L-Ala wild type enzyme, in 125 mM glycine/KOH (pH 10.2), at 25°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 38988
-
6 * 38988, predicted molecular mass Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1 Ni-NTA resin column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+
-
Mycobacterium tuberculosis pyruvate + NH3 + NADH
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.1 homohexamer in solution, gel filtration Mycobacterium tuberculosis
1.4.1.1 homohexamer 6 * 38988, predicted molecular mass Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.4.1.1 L-AlaDH
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.1 126
-
L-Ala wild type enzyme, in 125 mM glycine/KOH (pH 10.2), at 25°C Mycobacterium tuberculosis
1.4.1.1 694
-
pyruvate wild type enzyme, in 1 M NH4Cl/NH3, pH 7.4, at 25°C Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
Mycobacterium tuberculosis