EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.1.1 | expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.4.1.1 | hanging drop vapour diffusion method, crystals of apo-AlaDH are grown using 0.4 M ammonium hydrogenphosphate as reservoir solution at 20°C | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.1.1 | D270A | inactive | Mycobacterium tuberculosis |
1.4.1.1 | D270N | inactive, the bifurcated hydrogen bond between Asp270 and the ribose of NAD is replaced by a single hydrogen bond | Mycobacterium tuberculosis |
1.4.1.1 | H96A | inactive | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.1 | 0.76 | - |
pyruvate | wild type enzyme, in 1 M NH4Cl/NH3, pH 7.4, at 25°C | Mycobacterium tuberculosis | |
1.4.1.1 | 15.64 | - |
L-Ala | wild type enzyme, in 125 mM glycine/KOH (pH 10.2), at 25°C | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.1 | 38988 | - |
6 * 38988, predicted molecular mass | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.1 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.1 | Ni-NTA resin column chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.1 | L-Ala + H2O + NAD+ | - |
Mycobacterium tuberculosis | pyruvate + NH3 + NADH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.1 | homohexamer | in solution, gel filtration | Mycobacterium tuberculosis |
1.4.1.1 | homohexamer | 6 * 38988, predicted molecular mass | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.1 | L-AlaDH | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.1 | 126 | - |
L-Ala | wild type enzyme, in 125 mM glycine/KOH (pH 10.2), at 25°C | Mycobacterium tuberculosis | |
1.4.1.1 | 694 | - |
pyruvate | wild type enzyme, in 1 M NH4Cl/NH3, pH 7.4, at 25°C | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.1 | NAD+ | - |
Mycobacterium tuberculosis |