EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.29 | mutant expressed in Escherichia coli BL21(DE3) pLysS cells | Zymomonas mobilis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.2.29 | in complex with guanine, pre-queuine 0, and pre-queuine 1, hanging drop vapour diffusion method, in 100 mM MES, pH 5.5, 1 mM dithiothreitol, 13% (w/v) PEG 8000, 10% (v/v) dimethyl sulfoxide | Zymomonas mobilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.29 | E235Q | the mutation has no significant influence on kcat, but Km for preQ1 is drastically increased, while Km for preQ0 seems to be decreased | Zymomonas mobilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.29 | 0.0005 | - |
pre-queuine 0 | mutant enzyme E235Q, Km less than 0.0005 mM, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
2.4.2.29 | 0.0007 | - |
pre-queuine 1 | wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
2.4.2.29 | 0.0009 | - |
tRNA guanine | wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
2.4.2.29 | 0.0009 | - |
pre-queuine 0 | wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
2.4.2.29 | 0.001 | - |
tRNA guanine | mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
2.4.2.29 | 0.0012 | - |
guanine | wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
2.4.2.29 | 0.0033 | - |
guanine | mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
2.4.2.29 | 0.032 | - |
pre-queuine 1 | mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.29 | Zymomonas mobilis | P28720 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.29 | guanine34 in tRNA + 7-aminomethyl-7-carbaguanine | prefers pre-queuine 1 over pre-queuine 0 | Zymomonas mobilis | 7-aminomethyl-7-carbaguanine34 in tRNA + guanine | - |
? | |
2.4.2.29 | pre-queuine 0 + tRNA guanine | - |
Zymomonas mobilis | guanine + tRNA pre-queuine 0 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.29 | TGT | - |
Zymomonas mobilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.29 | 0.0006 | - |
pre-queuine 0 | mutant enzyme E235Q, Km less than 0.0005 mM | Zymomonas mobilis | |
2.4.2.29 | 0.012 | - |
pre-queuine 0 | wild type enzyme | Zymomonas mobilis | |
2.4.2.29 | 0.054 | - |
tRNA guanine | wild type enzyme | Zymomonas mobilis | |
2.4.2.29 | 0.056 | - |
guanine | wild type enzyme | Zymomonas mobilis | |
2.4.2.29 | 0.07 | - |
tRNA guanine | mutant enzyme E235Q | Zymomonas mobilis | |
2.4.2.29 | 0.076 | - |
guanine | mutant enzyme E235Q | Zymomonas mobilis | |
2.4.2.29 | 0.1 | - |
pre-queuine 1 | mutant enzyme E235Q | Zymomonas mobilis | |
2.4.2.29 | 0.102 | - |
pre-queuine 1 | wild type enzyme | Zymomonas mobilis |