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Literature summary extracted from

  • Klinke, S.; Zylberman, V.; Bonomi, H.R.; Haase, I.; Guimaraes, B.G.; Braden, B.C.; Bacher, A.; Fischer, M.; Goldbaum, F.A.
    Structural and kinetic properties of lumazine synthase isoenzymes in the order Rhizobiales (2007), J. Mol. Biol., 373, 664-680.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.78 expression in Escherichia coli Brucella abortus
2.5.1.78 expression in Escherichia coli Brucella melitensis
2.5.1.78 expression in Escherichia coli Mesorhizobium loti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.78 isoform RibH1, bound to the substrate analogue inhibitor 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. Comparison with structure of isoform RibH2 Brucella melitensis
2.5.1.78 isoform RibH1, unliganded, to 2.2 A resolution, and bound to the substrate analogue inhibitor 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. Comparison with structure of isoform RibH2 Brucella abortus
2.5.1.78 isoform RibH2, bound to the substrate analogue inhibitor 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione Mesorhizobium loti

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.78 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione crystallization data Brucella abortus
2.5.1.78 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione crystallization data Brucella melitensis
2.5.1.78 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione crystallization data; crystallization data Mesorhizobium loti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.78 0.0025
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione isoform RibH1, pH 7.0, 37°C Mesorhizobium loti
2.5.1.78 0.004
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C Brucella melitensis
2.5.1.78 0.015
-
 L-3,4-dihydroxybutan-2-one 4-phosphate isoform RibH1, pH 7.0, 37°C Mesorhizobium loti
2.5.1.78 0.02
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione isoform RibH2, pH 7.0, 37°C Mesorhizobium loti
2.5.1.78 0.225
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C Brucella melitensis
2.5.1.78 0.45
-
 L-3,4-dihydroxybutan-2-one 4-phosphate isoform RibH2, pH 7.0, 37°C Mesorhizobium loti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.78 16800
-
 5 * 16800, calculated, isoform RibH1 Brucella abortus
2.5.1.78 16800
-
 5 * 16800, calculated, isoform RibH1 Brucella melitensis
2.5.1.78 17200
-
 5 * 17200, calculated, isoform RibH1 Mesorhizobium loti
2.5.1.78 17300
-
 10 * 17300, calculated, isoform RibH2 Mesorhizobium loti
2.5.1.78 75300
-
 isoform RibH1, light scattering experiments Brucella melitensis
2.5.1.78 80100
-
 light scattering experiments, isoform RibH1 Brucella abortus
2.5.1.78 85500
-
 isoform RibH1, light scattering experiments Mesorhizobium loti
2.5.1.78 158500
-
 isoform RinH2, light scattering experiments Mesorhizobium loti

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.78 Brucella abortus
-
-
-
2.5.1.78 Brucella melitensis
-
 isoform RibH1
-
2.5.1.78 Mesorhizobium loti Q983B0
-
-
2.5.1.78 Mesorhizobium loti Q986N2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Brucella melitensis 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Mesorhizobium loti 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
2.5.1.78 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione + L-3,4-dihydroxybutan-2-one 4-phosphate
-
 Mesorhizobium loti 6,7-dimethyl-8-(1-D-ribityl)lumazine + 2 H2O + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.78 decamer 10 * 17300, calculated, isoform RibH2 Mesorhizobium loti
2.5.1.78 pentamer 5 * 16800, calculated, isoform RibH1 Brucella abortus
2.5.1.78 pentamer 5 * 16800, calculated, isoform RibH1 Brucella melitensis
2.5.1.78 pentamer 5 * 17200, calculated, isoform RibH1 Mesorhizobium loti

Synonyms

EC Number Synonyms Comment Organism
2.5.1.78 6,7-dimethyl-8-ribityllumazine synthase
-
 Brucella abortus
2.5.1.78 ribH1
-
 Mesorhizobium loti
2.5.1.78 RibH2
-
 Mesorhizobium loti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.78 37
-
 assay at Brucella melitensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.78 0.0003
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione isoform RibH2, pH 7.0, 37°C Mesorhizobium loti
2.5.1.78 0.003
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C Brucella melitensis
2.5.1.78 0.04
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione isoform RibH1, pH 7.0, 37°C Mesorhizobium loti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.78 7
-
 assay at Brucella melitensis