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Literature summary extracted from

  • Kim, J.H.; Sung, M.W.; Lee, E.H.; Nam, K.H.; Hwang, K.Y.
    Crystallization and preliminary X-ray diffraction analysis of 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum DSM 1728 (2008), J. Microbiol. Biotechnol., 18, 283-286.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.2.13 NAD+
-
 Thermoplasma acidophilum
3.5.2.13 NADP+
-
 Thermoplasma acidophilum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.5 expressed in Escherichia coli Thermoplasma acidophilum
3.5.2.13 expressed in Escherichia coli strain BL21 (DE3) Thermoplasma acidophilum
3.5.4.9 expressed in Escherichia coli strain BL21 (DE3) Thermoplasma acidophilum DSM 1728

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.5 hanging drop vapour diffusion method, in the presence of its cofactor NADP+, at 22°C using polyethylene glycol 4000 as a precipitant Thermoplasma acidophilum
3.5.2.13 in the presence of NADP+, at 22°C, using polyethylene glycol 4000 as a precipitant, sitting drop vapour diffusion method Thermoplasma acidophilum
3.5.4.9 in the presence of NADP+, at 22°C, using polyethylene glycol 4000 as a precipitant, sitting drop vapour diffusion method Thermoplasma acidophilum DSM 1728

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.5 30600
-
 SDS-PAGE Thermoplasma acidophilum
3.5.2.13 30600
-
 SDS-PAGE Thermoplasma acidophilum
3.5.4.9 30600
-
 estimated from amino acid sequence Thermoplasma acidophilum DSM 1728

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.5 Thermoplasma acidophilum
-
 strain DSM 1728
-
3.5.2.13 Thermoplasma acidophilum
-
 strain DSM 1728
-
3.5.4.9 Thermoplasma acidophilum DSM 1728
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.5 His-Trap affinity column chromatography Thermoplasma acidophilum
3.5.2.13 His-Trap affinity column chromatography and Superdex-S200 gel filtration Thermoplasma acidophilum
3.5.4.9 His-Trap affinity column chromatography and Superdex-S200 gel filtration Thermoplasma acidophilum DSM 1728

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+
-
 Thermoplasma acidophilum 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
3.5.2.13 formyltetrahydrofolate + H2O
-
 Thermoplasma acidophilum methylenetetrahydrofolate + ?
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Thermoplasma acidophilum DSM 1728 10-formyltetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.5 monomer 1 * 30600, SDS-PAGE Thermoplasma acidophilum
3.5.2.13 dimer x-ray crystallography Thermoplasma acidophilum
3.5.4.9 dimer x-ray crystallography Thermoplasma acidophilum DSM 1728

Synonyms

EC Number Synonyms Comment Organism
1.5.1.5 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase
-
 Thermoplasma acidophilum
1.5.1.5 MTHFDC
-
 Thermoplasma acidophilum
3.5.2.13 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase
-
 Thermoplasma acidophilum
3.5.2.13 MTHFDC
-
 Thermoplasma acidophilum
3.5.4.9 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase
-
 Thermoplasma acidophilum DSM 1728
3.5.4.9 MTHFDC
-
 Thermoplasma acidophilum DSM 1728

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.5 NAD+ prefers NADP+ as a cofactor, rather than NAD+ Thermoplasma acidophilum
1.5.1.5 NADP+ prefers NADP+ as a cofactor, rather than NAD+ Thermoplasma acidophilum
3.5.4.9 additional information preference for NADP+ as a cofactor, rather than NAD+ Thermoplasma acidophilum DSM 1728