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Literature summary extracted from

  • Rosini, E.; Molla, G.; Rossetti, C.; Pilone, M.S.; Pollegioni, L.; Sacchi, S.
    A biosensor for all D-amino acids using evolved D-amino acid oxidase (2008), J. Biotechnol., 135, 377-384.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 expressed in Escherichia coli BL21(DE3)pLysS cells Rhodotorula toruloides

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.3 M213E increased overall activity compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 M213G increased overall activity compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 M213R increased overall activity compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 Q144R increased overall activity compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 Q339E decreased overall activity compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 T60A/Q144R/K152E increased overall activity compared to the wild type enzyme Rhodotorula toruloides
1.4.3.3 Y238R increased overall activity compared to the wild type enzyme Rhodotorula toruloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.3
-
 D-methionine mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 0.6
-
 D-alanine mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 0.6
-
 D-methionine wild type enzyme, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 0.8
-
 D-methionine mutant enzyme M213G, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 0.9
-
 D-alanine wild type enzyme, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 1.2
-
 D-methionine mutant enzyme M213R, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 1.3
-
 D-valine immobilized wild type enzyme, at 25°C Rhodotorula toruloides
1.4.3.3 2.5
-
 D-glutamine mutant enzyme M213G, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 4.9
-
 D-alanine mutant enzyme M213G, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 5.1
-
 D-glutamate mutant enzyme M213G, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 5.4
-
 D-glutamine mutant enzyme M213R, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 5.4
-
 D-glutamine wild type enzyme, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 5.6
-
 D-proline immobilized wild type enzyme, at 25°C Rhodotorula toruloides
1.4.3.3 6
-
 D-glutamine mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 8
-
 D-Lysine mutant enzyme M213G, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 12.5
-
 D-proline free wild type enzyme, at 25°C Rhodotorula toruloides
1.4.3.3 14.6
-
 D-Lysine wild type enzyme, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 16.6
-
 D-Lysine mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 18.5
-
 D-alanine mutant enzyme M213R, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 18.9
-
 D-valine free wild type enzyme, at 25°C Rhodotorula toruloides
1.4.3.3 21.1
-
 D-glutamate mutant enzyme M213R, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 76
-
 D-glutamate wild type enzyme, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.4.3.3 101
-
 D-glutamate mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Rhodotorula toruloides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3 Hitrap chelating chromatography Rhodotorula toruloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 cephalosporin C + H2O + O2
-
 Rhodotorula toruloides 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-alanine + H2O + O2
-
 Rhodotorula toruloides pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-arginine + H2O + O2
-
 Rhodotorula toruloides 5-guanidino-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-asparagine + H2O + O2
-
 Rhodotorula toruloides 2-oxosuccinamic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-aspartate + H2O + O2
-
 Rhodotorula toruloides oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.3 D-cysteine + H2O + O2
-
 Rhodotorula toruloides 2-oxo-3-thiopropionic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-glutamate + H2O + O2
-
 Rhodotorula toruloides alpha-ketoglutarate + NH3 + H2O2
-
 ?
1.4.3.3 D-glutamine + H2O + O2
-
 Rhodotorula toruloides 2-oxoglutaramate + NH3 + H2O2
-
 ?
1.4.3.3 D-histidine + H2O + O2
-
 Rhodotorula toruloides 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-isoleucine + H2O + O2
-
 Rhodotorula toruloides 3-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-leucine + H2O + O2
-
 Rhodotorula toruloides 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-lysine + H2O + O2
-
 Rhodotorula toruloides 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2
-
 Rhodotorula toruloides 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2
-
 Rhodotorula toruloides phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-proline + H2O + O2
-
 Rhodotorula toruloides 2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-serine + H2O + O2
-
 Rhodotorula toruloides 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-threonine + H2O + O2
-
 Rhodotorula toruloides 2-oxo-3-hydroxybutyric acid + NH3 + H2O2
-
 ?
1.4.3.3 D-tryptophan + H2O + O2
-
 Rhodotorula toruloides indol-3-pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-tyrosine + H2O + O2
-
 Rhodotorula toruloides 4-hydroxyphenylpyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-valine + H2O + O2
-
 Rhodotorula toruloides alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?
1.4.3.3 glycine + 2 H2O + O2
-
 Rhodotorula toruloides 2 formic acid + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 D-amino acid oxidase
-
 Rhodotorula toruloides
1.4.3.3 DAAO
-
 Rhodotorula toruloides

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD FAD-containing flavoenzyme Rhodotorula toruloides