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Literature summary extracted from

  • Isobe, K.; Kato, A.; Sasaki, Y.; Suzuki, S.; Kataoka, M.; Ogawa, J.; Iwasaki, A.; Hasegawa, J.; Shimizu, S.
    Purification and characterization of a novel alcohol oxidase from Paenibacillus sp. AIU 311 (2007), J. Biosci. Bioeng., 104, 124-128.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.9 2,2'-dipyridyl 1 mM, 67% inhibition Paenibacillus sp. AIU 311
1.1.3.9 8-hydroxyquinoline 1 mM, 50% inhibition Paenibacillus sp. AIU 311
1.1.3.9 Co2+ 1 mM, 50% inhibition Paenibacillus sp. AIU 311
1.1.3.9 EDTA 1 mM, 34% inhibition Paenibacillus sp. AIU 311
1.1.3.9 Fe2+ 1 mM, 17% inhibition Paenibacillus sp. AIU 311
1.1.3.9 hydrazine 1 mM, 41% inhibition Paenibacillus sp. AIU 311
1.1.3.9 hydroxylamine 1 mM, 31% inhibition Paenibacillus sp. AIU 311
1.1.3.9 Mg2+ 1 mM, 50% inhibition Paenibacillus sp. AIU 311
1.1.3.9 Monoiodoacetic acid 1 mM, 40% inhibition Paenibacillus sp. AIU 311
1.1.3.9 Ni2+ 1 mM, 50% inhibition Paenibacillus sp. AIU 311
1.1.3.9 o-phenanthroline 1 mM, 19% inhibition Paenibacillus sp. AIU 311
1.1.3.9 phenylhydrazine 1 mM, 91% inhibition Paenibacillus sp. AIU 311
1.1.3.9 Semicarbazide 1 mM, 44% inhibition Paenibacillus sp. AIU 311
1.1.3.9 Zn2+ 1 mM, 33% inhibition Paenibacillus sp. AIU 311

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.9 7.5
-
 glyceraldehyde
-
 Paenibacillus sp. AIU 311
1.1.3.9 13.2
-
 glycolaldehyde
-
 Paenibacillus sp. AIU 311

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.9 Mn2+ 1 mM, 1.6fold activation Paenibacillus sp. AIU 311

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.9 24000
-
 2 * 24000, SDS-PAGE Paenibacillus sp. AIU 311
1.1.3.9 49000
-
 gel filtration Paenibacillus sp. AIU 311

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.9 Paenibacillus sp. AIU 311
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.9
-
 Paenibacillus sp. AIU 311

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.9 0.807
-
-
 Paenibacillus sp. AIU 311

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.9 glyceraldehyde + O2 70% of the activity with glycolaldehyde Paenibacillus sp. AIU 311 ? + H2O2
-
 ?
1.1.3.9 glycolaldehyde + O2
-
 Paenibacillus sp. AIU 311 glyoxal + H2O2
-
 ?
1.1.3.9 additional information no activity with methanol, ethanol, 1-propanol, 1-butanol, 2-propanol, 2-butanol, 2-methoxyethanol, 1,2-propanediol, 1,3-propanediol, glycerol, glyoxylic acid, D-arabinose, D-ribose, D-lyxose, isobutyraldehyde, valeraldehyde, methylglyoxal, benzaldehyde Paenibacillus sp. AIU 311 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.9 dimer 2 * 24000, SDS-PAGE Paenibacillus sp. AIU 311

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.9 50
-
 oxidation of glycolaldehyde Paenibacillus sp. AIU 311

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.9 40 60 40°C: about 65% of maximal activity, 60°C: about 80% of maximal activity, oxidation of glycolaldehyde Paenibacillus sp. AIU 311

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.9 30
-
 pH 6.5, 60 min, stable Paenibacillus sp. AIU 311
1.1.3.9 40
-
 pH 6.5, 60 min, about 10% loss of activity Paenibacillus sp. AIU 311
1.1.3.9 50
-
 pH 6.5, 60 min, about 20% loss of activity Paenibacillus sp. AIU 311
1.1.3.9 60
-
 pH 6.5, 60 min, about 30% loss of activity Paenibacillus sp. AIU 311
1.1.3.9 70
-
 pH 6.5, 60 min, about 40% loss of activity Paenibacillus sp. AIU 311

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.9 6.5
-
 oxidation of glycolaldehyde Paenibacillus sp. AIU 311

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.9 6 7 pH 6: about 80% of maximal activity, pH 7: about 25% loss of activity, oxidation of glycolaldehyde Paenibacillus sp. AIU 311

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.9 5
-
 40°C, 60 min, stable Paenibacillus sp. AIU 311
1.1.3.9 6
-
 40°C, 60 min, about 5% loss of activity Paenibacillus sp. AIU 311
1.1.3.9 7
-
 40°C, 60 min, about 10% of maximal activity Paenibacillus sp. AIU 311
1.1.3.9 8
-
 40°C, 60 min, about 20% of maximal activity Paenibacillus sp. AIU 311

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.9 Paenibacillus sp. AIU 311 isoelectric focusing, pH-range: 3.5-10
-
 3.2