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Literature summary extracted from

  • Xia, Z.X.; Dai, W.W.; He, Y.N.; White, S.A.; Mathews, F.S.; Davidson, V.L.
    X-ray structure of methanol dehydrogenase from Paracoccus denitrificans and molecular modeling of its interactions with cytochrome c-551i (2003), J. Biol. Inorg. Chem., 8, 843-854.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.2.B2 purified enzyme, hanging drop vapor diffusion and macro-seeding, mixing of protein solution containing 5 mg/ml protein, 0.1 M Tris-HCl, pH 8.3, 0.2 M Li2SO4, 30% PEG 3350, with precipitant solution containing 0.1 M Tris-HCl, pH 8.5, 30% PEG 4000, 0.2 M Li2SO4, several days, X-ray diffraction structure determination and analysis at 2.5 A resolution, modelling Paracoccus denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.B2 Ca2+
-
 Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.B2 methanol + ferricytochrome c551i Paracoccus denitrificans the primary electron acceptor of MEDH-PD is cytochrome c-551i formaldehyde + ferrocytochrome c551i
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.B2 Paracoccus denitrificans P12293 P12293 (large subunit, alpha) and P12293 (small subunit, beta)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.B2 methanol + ferricytochrome c551i the primary electron acceptor of MEDH-PD is cytochrome c-551i Paracoccus denitrificans formaldehyde + ferrocytochrome c551i
-
 ?
1.1.2.B2 additional information interaction of the enzyme with cytochrome c551i, and electron transfer from reduced pyrroloquinoline quinone to the cytochrome, interface structure, modelling, overview Paracoccus denitrificans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.B2 MEDH
-
 Paracoccus denitrificans
1.1.2.B2 MEDH-PD
-
 Paracoccus denitrificans
1.1.2.B2 methanol dehydrogenase
-
 Paracoccus denitrificans
1.1.5.5 MEDH
-
-
1.1.5.5 MEDH-PD
-
-
1.1.5.11 MEDH-PD Cyt c551i
-
-

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.B2 pyrroloquinoline quinone
-
 Paracoccus denitrificans