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Literature summary extracted from

  • Szegedi, S.S.; Castro, C.C.; Koutmos, M.; Garrow, T.A.
    Betaine-homocysteine S-methyltransferase-2 is an S-methylmethionine-homocysteine methyltransferase (2008), J. Biol. Chem., 283, 8939-8945.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.10 expressed in Escherichia coli, recombinant protein, intein/chitin-binding fusion constructs, pTBY3 vector Homo sapiens

General Stability

EC Number General Stability Organism
2.1.1.10 high concentrations of glycerol (40%, v/v) greatly stabilized activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.5 AdoMet weak inhibitor, at 2 mM: 24% inhibition Homo sapiens
2.1.1.5 AdoMet weak inhibitor, at 2.5 mM: 15% inhibition Mus musculus
2.1.1.5 dimethylglycine at 0.2 mM: 80% inhibition, at 2 mM: 97% inhibition; weak inhibitor, at 2 mM: 11% inhibition Homo sapiens
2.1.1.5 dimethylglycine at 0.25 mM: 64% inhibition, at 2.5 mM: 95% inhibition; weak inhibitor, at 2.5 mM: 19% inhibition Mus musculus
2.1.1.5 dimethylsulfonioacetate at 2 mM: 79% inhibition; weak inhibitor, at 2 mM: 20% inhibition Homo sapiens
2.1.1.5 dimethylsulfonioacetate at 0.25 mM: 29% inhibition, at 2.5 mM: 82% inhibition Mus musculus
2.1.1.5 dimethylsulfoniopropionate weak inhibitor, at 2 mM: 29% inhibition Homo sapiens
2.1.1.5 dimethylsulfoniopropionate weak inhibitor, at 2.5 mM: 11% inhibition; weak inhibitor, at 2.5 mM: 17% inhibition Mus musculus
2.1.1.5 methionine at 2 mM: 60% inhibition; weak inhibitor at 2 mM: 15% inhibition Homo sapiens
2.1.1.5 methionine at 2.5 mM: 38% inhibition; at 2.5 mM: 48% inhibition Mus musculus
2.1.1.5 S-(delta-carboxybutyl)-L-homocysteine at 0.05 mM: 97% inhibition, at 0.5 mM: total inhibition; weak inhibitor, at 0.5 mM: 26% inhibition Homo sapiens
2.1.1.5 S-(delta-carboxybutyl)-L-homocysteine at 0.025 mM: 22% inhibition, at 0.0625 mM: 36% inhibition, at 0.125 mM: 49% inhibition, at 0.5 mM: 81% inhibition; total inhibition Mus musculus
2.1.1.10 L-methionine stronger inhibitor of BHMT-2 than of BHMT (EC 2.1.1.5) Homo sapiens
2.1.1.10 additional information relative inhibition of human BHMT (EC 2.1.1.5) and human BHMT-2 activities by methyl donor substrates determined Homo sapiens
2.1.1.10 S-adenosyl-L-methionine weak inhibitor of, does not inhibit BHMT (EC 2.1.1.5), not inhibited by betaine Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.5 0.76
-
S-methyl-L-methionine
-
Mus musculus
2.1.1.5 0.94
-
S-methyl-L-methionine
-
Homo sapiens
2.1.1.5 2.2
-
betaine
-
Homo sapiens
2.1.1.5 3
-
S-methyl-L-methionine
-
Homo sapiens
2.1.1.10 0.94
-
S-methyl-L-methionine final concentrations of S-methyl-L-methionine ranging from 0.1 mM to 9 mM, lower value than obtained for BHMT (EC 2.1.1.5) Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.5 Zn BHMT-2 is a zinc metalloenzyme Mus musculus
2.1.1.5 Zn BHMT-2 is a zinc metalloenzyme Homo sapiens
2.1.1.5 Zn2+ BHMT is a zinc metalloenzyme Homo sapiens
2.1.1.5 Zn2+ BHMT-2 is a zinc metalloenzyme Mus musculus
2.1.1.10 Zn2+ required for activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.10 40000
-
SDS-PAGE, 73% sequence identity with the 45 kDa BHMT protein (EC 2.1.1.5) Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.5 Homo sapiens
-
-
-
2.1.1.5 Mus musculus
-
-
-
2.1.1.10 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.10 gel filtration, recombinant protein Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.10 S-methyl-L-methionine + L-homocysteine = 2 L-methionine high homology to betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5), betaine is not a substrate for BHMT-2 but high activity with S-methyl-L-methionine Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.5 kidney
-
Mus musculus
-
2.1.1.5 liver
-
Mus musculus
-
2.1.1.10 kidney tissue extracts, activities measured in Homo sapiens
-
2.1.1.10 liver tissue extracts, activities measured in Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.10 additional information
-
structural comparison and enzymatic properties of purified human betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5) and human betaine-homocysteine methyltransferase-2 (BHMT-2) determined, methylation capacities of homocysteine analyzed, S-adenosyl-L-methionine-dependent methylation of homocysteine predicted mostly occurs via BHMT-2 in vivo Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.5 L-homocysteine + betaine
-
Mus musculus L-methionine + dimethylglycine
-
?
2.1.1.5 L-homocysteine + betaine
-
Homo sapiens L-methionine + dimethylglycine
-
?
2.1.1.5 L-homocysteine + S-methyl-L-methionine
-
Mus musculus ?
-
?
2.1.1.5 L-homocysteine + S-methyl-L-methionine
-
Homo sapiens ?
-
?
2.1.1.5 L-homocysteine + S-methyl-L-methionine BHMT-2 uses S-methylmethionine as a methyl donor for the methylation of homocysteine. Unlike BHMT, BHMT-2 can not use betaine Homo sapiens ?
-
?
2.1.1.5 L-homocysteine + S-methylmethionine BHMT-2 uses S-methylmethionine as a methyl donor for the methylation of homocysteine. Unlike BHMT, BHMT-2 can not use betaine Mus musculus ?
-
?
2.1.1.10 S-adenosyl-L-methionine + L-homocysteine low activity Homo sapiens ?
-
?
2.1.1.10 S-methyl-L-methionine + L-homocysteine high homology to betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5), betaine is not a substrate for BHMT-2 but high activity with S-methyl-L-methionine Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.5 betaine-homocysteine S-methyltransferase
-
Mus musculus
2.1.1.5 betaine-homocysteine S-methyltransferase
-
Homo sapiens
2.1.1.5 betaine-homocysteine S-methyltransferase-2
-
Mus musculus
2.1.1.5 betaine-homocysteine S-methyltransferase-2
-
Homo sapiens
2.1.1.5 BHMT
-
Mus musculus
2.1.1.5 BHMT
-
Homo sapiens
2.1.1.5 BHMT-2
-
Homo sapiens
2.1.1.5 BHMT2
-
Mus musculus
2.1.1.10 BHMT-2
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.10 7.5
-
activity assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.5 24
-
S-methyl-L-methionine
-
Homo sapiens
2.1.1.5 38
-
S-methyl-L-methionine
-
Homo sapiens
2.1.1.5 88
-
betaine
-
Homo sapiens
2.1.1.10 additional information
-
additional information similar turnover number like betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5) Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.5 7.5
-
assay at Mus musculus