EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.10 | expressed in Escherichia coli, recombinant protein, intein/chitin-binding fusion constructs, pTBY3 vector | Homo sapiens |
EC Number | General Stability | Organism |
---|---|---|
2.1.1.10 | high concentrations of glycerol (40%, v/v) greatly stabilized activity | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.5 | AdoMet | weak inhibitor, at 2 mM: 24% inhibition | Homo sapiens | |
2.1.1.5 | AdoMet | weak inhibitor, at 2.5 mM: 15% inhibition | Mus musculus | |
2.1.1.5 | dimethylglycine | at 0.2 mM: 80% inhibition, at 2 mM: 97% inhibition; weak inhibitor, at 2 mM: 11% inhibition | Homo sapiens | |
2.1.1.5 | dimethylglycine | at 0.25 mM: 64% inhibition, at 2.5 mM: 95% inhibition; weak inhibitor, at 2.5 mM: 19% inhibition | Mus musculus | |
2.1.1.5 | dimethylsulfonioacetate | at 2 mM: 79% inhibition; weak inhibitor, at 2 mM: 20% inhibition | Homo sapiens | |
2.1.1.5 | dimethylsulfonioacetate | at 0.25 mM: 29% inhibition, at 2.5 mM: 82% inhibition | Mus musculus | |
2.1.1.5 | dimethylsulfoniopropionate | weak inhibitor, at 2 mM: 29% inhibition | Homo sapiens | |
2.1.1.5 | dimethylsulfoniopropionate | weak inhibitor, at 2.5 mM: 11% inhibition; weak inhibitor, at 2.5 mM: 17% inhibition | Mus musculus | |
2.1.1.5 | methionine | at 2 mM: 60% inhibition; weak inhibitor at 2 mM: 15% inhibition | Homo sapiens | |
2.1.1.5 | methionine | at 2.5 mM: 38% inhibition; at 2.5 mM: 48% inhibition | Mus musculus | |
2.1.1.5 | S-(delta-carboxybutyl)-L-homocysteine | at 0.05 mM: 97% inhibition, at 0.5 mM: total inhibition; weak inhibitor, at 0.5 mM: 26% inhibition | Homo sapiens | |
2.1.1.5 | S-(delta-carboxybutyl)-L-homocysteine | at 0.025 mM: 22% inhibition, at 0.0625 mM: 36% inhibition, at 0.125 mM: 49% inhibition, at 0.5 mM: 81% inhibition; total inhibition | Mus musculus | |
2.1.1.10 | L-methionine | stronger inhibitor of BHMT-2 than of BHMT (EC 2.1.1.5) | Homo sapiens | |
2.1.1.10 | additional information | relative inhibition of human BHMT (EC 2.1.1.5) and human BHMT-2 activities by methyl donor substrates determined | Homo sapiens | |
2.1.1.10 | S-adenosyl-L-methionine | weak inhibitor of, does not inhibit BHMT (EC 2.1.1.5), not inhibited by betaine | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.5 | 0.76 | - |
S-methyl-L-methionine | - |
Mus musculus | |
2.1.1.5 | 0.94 | - |
S-methyl-L-methionine | - |
Homo sapiens | |
2.1.1.5 | 2.2 | - |
betaine | - |
Homo sapiens | |
2.1.1.5 | 3 | - |
S-methyl-L-methionine | - |
Homo sapiens | |
2.1.1.10 | 0.94 | - |
S-methyl-L-methionine | final concentrations of S-methyl-L-methionine ranging from 0.1 mM to 9 mM, lower value than obtained for BHMT (EC 2.1.1.5) | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.5 | Zn | BHMT-2 is a zinc metalloenzyme | Mus musculus | |
2.1.1.5 | Zn | BHMT-2 is a zinc metalloenzyme | Homo sapiens | |
2.1.1.5 | Zn2+ | BHMT is a zinc metalloenzyme | Homo sapiens | |
2.1.1.5 | Zn2+ | BHMT-2 is a zinc metalloenzyme | Mus musculus | |
2.1.1.10 | Zn2+ | required for activity | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.10 | 40000 | - |
SDS-PAGE, 73% sequence identity with the 45 kDa BHMT protein (EC 2.1.1.5) | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.5 | Homo sapiens | - |
- |
- |
2.1.1.5 | Mus musculus | - |
- |
- |
2.1.1.10 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.10 | gel filtration, recombinant protein | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.1.1.10 | S-methyl-L-methionine + L-homocysteine = 2 L-methionine | high homology to betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5), betaine is not a substrate for BHMT-2 but high activity with S-methyl-L-methionine | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.5 | kidney | - |
Mus musculus | - |
2.1.1.5 | liver | - |
Mus musculus | - |
2.1.1.10 | kidney | tissue extracts, activities measured in | Homo sapiens | - |
2.1.1.10 | liver | tissue extracts, activities measured in | Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.10 | additional information | - |
structural comparison and enzymatic properties of purified human betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5) and human betaine-homocysteine methyltransferase-2 (BHMT-2) determined, methylation capacities of homocysteine analyzed, S-adenosyl-L-methionine-dependent methylation of homocysteine predicted mostly occurs via BHMT-2 in vivo | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.5 | L-homocysteine + betaine | - |
Mus musculus | L-methionine + dimethylglycine | - |
? | |
2.1.1.5 | L-homocysteine + betaine | - |
Homo sapiens | L-methionine + dimethylglycine | - |
? | |
2.1.1.5 | L-homocysteine + S-methyl-L-methionine | - |
Mus musculus | ? | - |
? | |
2.1.1.5 | L-homocysteine + S-methyl-L-methionine | - |
Homo sapiens | ? | - |
? | |
2.1.1.5 | L-homocysteine + S-methyl-L-methionine | BHMT-2 uses S-methylmethionine as a methyl donor for the methylation of homocysteine. Unlike BHMT, BHMT-2 can not use betaine | Homo sapiens | ? | - |
? | |
2.1.1.5 | L-homocysteine + S-methylmethionine | BHMT-2 uses S-methylmethionine as a methyl donor for the methylation of homocysteine. Unlike BHMT, BHMT-2 can not use betaine | Mus musculus | ? | - |
? | |
2.1.1.10 | S-adenosyl-L-methionine + L-homocysteine | low activity | Homo sapiens | ? | - |
? | |
2.1.1.10 | S-methyl-L-methionine + L-homocysteine | high homology to betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5), betaine is not a substrate for BHMT-2 but high activity with S-methyl-L-methionine | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.5 | betaine-homocysteine S-methyltransferase | - |
Mus musculus |
2.1.1.5 | betaine-homocysteine S-methyltransferase | - |
Homo sapiens |
2.1.1.5 | betaine-homocysteine S-methyltransferase-2 | - |
Mus musculus |
2.1.1.5 | betaine-homocysteine S-methyltransferase-2 | - |
Homo sapiens |
2.1.1.5 | BHMT | - |
Mus musculus |
2.1.1.5 | BHMT | - |
Homo sapiens |
2.1.1.5 | BHMT-2 | - |
Homo sapiens |
2.1.1.5 | BHMT2 | - |
Mus musculus |
2.1.1.10 | BHMT-2 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.10 | 7.5 | - |
activity assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.5 | 24 | - |
S-methyl-L-methionine | - |
Homo sapiens | |
2.1.1.5 | 38 | - |
S-methyl-L-methionine | - |
Homo sapiens | |
2.1.1.5 | 88 | - |
betaine | - |
Homo sapiens | |
2.1.1.10 | additional information | - |
additional information | similar turnover number like betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5) | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.5 | 7.5 | - |
assay at | Mus musculus |