Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ghosh, A.K.; Ramakrishnan, G.; Chandramohan, C.; Rajasekharan, R.
    CGI-58, the causative gene for Chanarin-Dorfman syndrome, mediates acylation of lysophosphatidic acid (2008), J. Biol. Chem., 283, 24525-24533.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.51 overexpressed in Escherichia coli BL21 (DE3), pCMV-SPORT6 vector, overexpressed in Saccharomyces cerevisiae, pYES2 vector Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.51 E260K site-directed mutagenesis, mutation found in Chanarin-Dorfman syndrome patients, mutation without significant effect on acyltransferase activity Homo sapiens
2.3.1.51 Q130P site-directed mutagenesis, mutation found in Chanarin-Dorfman syndrome patients, mutation without significant effect on acyltransferase activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.51 lysophosphatidylcholine inhibits 68% of the enzyme activity at 30 microM Homo sapiens
2.3.1.51 Phospholipids activity inhibited by high concentrations of Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.51 0.012
-
 palmitoyl-CoA stearoyl-CoA as an acyl donor reveals only 33% of the activity observed under the standard assay conditions Homo sapiens
2.3.1.51 0.014
-
 oleoyl-CoA
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.51 cytosol white adipose tissue of mice, precise role of cgi-58 protein in the cytoplasm unknown Homo sapiens 5829
-
2.3.1.51 lipid droplet white adipose tissue of mice Homo sapiens 5811
-
2.3.1.51 membrane more than 60% of total activity associated with membranes in white adipose tissue Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.51 additional information
-
 45 kDa protein in Western Blot and mass spectrometry analysis, subcellular fractions of white adipose tissue Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Homo sapiens
-
 CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.51 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.51 gel filtration and SDS-PAGE, recombinant protein, mutant and wild-type Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.51 white adipose tissue physiological significance analyzed in white adipose tissue of mice, soluble lysophosphatidic acid acyltransferase activity, existence of an alternate cytosolic phosphatidic acid biosynthetic pathway Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Homo sapiens CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate substrate preference analyzed, assays performed with different acyl donors Homo sapiens CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 oleoyl-CoA + 1-oleoyl-sn-glycerol-3-phosphate substrate preference higher for oleoyl-CoA than for palmitoyl-CoA and stearoyl-CoA Homo sapiens CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Homo sapiens CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.51 cgi-58 protein
-
 Homo sapiens
2.3.1.51 lysophosphatidic acid ayltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.51 30
-
 activity assay at Homo sapiens