EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.173 | gene LRA1, DNA and amino acid sequence determination and analysis, genetic organization, functional expression of His-tagged enzyme in Escherichia coli | Debaryomyces hansenii |
1.1.1.173 | gene LRA1, DNA and amino acid sequence determination and analysis, genetic organization, functional expression of His-tagged enzyme in Escherichia coli | Scheffersomyces stipitis |
1.1.1.378 | expression in Escherichia coli | Azotobacter vinelandii |
3.1.1.65 | overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme | Azotobacter vinelandii |
3.1.1.65 | overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme | Debaryomyces hansenii |
3.1.1.65 | overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme | Scheffersomyces stipitis |
4.1.2.53 | expressed in Escherichia coli DH5alpha cells | Azotobacter vinelandii |
4.1.2.53 | expressed in Escherichia coli DH5alpha cells | Scheffersomyces stipitis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.53 | Ca2+ | complete inhibition in the presence of Ca2+ | Azotobacter vinelandii | |
4.1.2.53 | Co2+ | about 75% residual activity in the presence of Co2+ | Azotobacter vinelandii | |
4.1.2.53 | Cu2+ | complete inhibition in the presence of Cu2+ | Azotobacter vinelandii | |
4.1.2.53 | Fe2+ | complete inhibition in the presence of Fe2+ | Azotobacter vinelandii | |
4.1.2.53 | Mg2+ | about 25% residual activity in the presence of Mg2+ | Azotobacter vinelandii | |
4.1.2.53 | Ni2+ | about 10% residual activity in the presence of Ni2+ | Azotobacter vinelandii | |
4.1.2.53 | Zn2+ | about 80% residual activity in the presence of Zn2+ | Azotobacter vinelandii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.173 | 1.71 | - |
L-rhamnose | pH 9.0, 30°C, recombinant enzyme | Scheffersomyces stipitis | |
1.1.1.173 | 9.35 | - |
L-rhamnose | pH 9.0, 30°C, recombinant enzyme | Debaryomyces hansenii | |
1.1.1.378 | 2.34 | - |
L-rhamnose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
1.1.1.378 | 2.61 | - |
L-rhamnose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
1.1.1.378 | 4.96 | - |
L-Lyxose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
1.1.1.378 | 8.43 | - |
L-Lyxose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
1.1.1.378 | 89.3 | - |
L-Mannose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
1.1.1.378 | 108 | - |
L-Mannose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
1.1.1.378 | 582 | - |
L-fucose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
1.1.1.378 | 673 | - |
L-fucose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
4.1.2.53 | 0.915 | - |
2-dehydro-3-deoxy-L-rhamnonate | in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication | Azotobacter vinelandii | |
4.1.2.53 | 1.37 | - |
2-dehydro-3-deoxy-L-rhamnonate | in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication | Scheffersomyces stipitis | |
4.2.1.90 | 0.115 | - |
L-Rhamnonate | pH 7.0, 30°C | Azotobacter vinelandii | |
4.2.1.90 | 1.37 | - |
L-Rhamnonate | pH 7.0, 30°C | Scheffersomyces stipitis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.53 | Co2+ | about 240% activity in the presence of Co2+ | Scheffersomyces stipitis | |
4.1.2.53 | Mg2+ | about 150% activity in the presence of Co2+ | Scheffersomyces stipitis | |
4.1.2.53 | Mg2+ | more than 250% activity in the presence of Mg2+, 5 mM MgCl2 used in assay conditions | Scheffersomyces stipitis | |
4.1.2.53 | additional information | not stimulated by Mg2+, Co2+, Ni2+, Zn2+, Fe2+, Cu2+, and Ca2+ | Azotobacter vinelandii | |
4.1.2.53 | additional information | not stimulated by Zn2+, Fe2+, Cu2+, and Ca2+ | Scheffersomyces stipitis | |
4.1.2.53 | Ni2+ | about 140% activity in the presence of Ni2+ | Scheffersomyces stipitis | |
4.2.1.90 | Mg2+ | - |
Azotobacter vinelandii | |
4.2.1.90 | Mg2+ | - |
Debaryomyces hansenii | |
4.2.1.90 | Mg2+ | - |
Scheffersomyces stipitis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.173 | 25000 | - |
x * 25000, SDS-PAGE | Scheffersomyces stipitis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.173 | L-rhamnose + NAD+ | Debaryomyces hansenii | - |
L-rhamno-1,4-lactone + NADH | - |
r | |
1.1.1.173 | L-rhamnose + NAD+ | Scheffersomyces stipitis | - |
L-rhamno-1,4-lactone + NADH | - |
r | |
1.1.1.173 | L-rhamnose + NAD+ | Debaryomyces hansenii NBRC0083 | - |
L-rhamno-1,4-lactone + NADH | - |
r | |
1.1.1.378 | L-rhamnose + NAD(P)+ | Azotobacter vinelandii | first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway | L-rhamno-1,4-lactone + NAD(P)H + H+ | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | Azotobacter vinelandii | - |
L-rhamnonate | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | Debaryomyces hansenii | - |
L-rhamnonate | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | Scheffersomyces stipitis | - |
L-rhamnonate | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | Debaryomyces hansenii NBRC0083 | - |
L-rhamnonate | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | Azotobacter vinelandii NBRC 102612 | - |
L-rhamnonate | - |
? | |
4.1.2.53 | 2-dehydro-3-deoxy-L-rhamnonate | Azotobacter vinelandii | preferred substrate | pyruvate + (R)-lactaldehyde | - |
? | |
4.1.2.53 | 2-dehydro-3-deoxy-L-rhamnonate | Scheffersomyces stipitis | preferred substrate | pyruvate + (R)-lactaldehyde | - |
? | |
4.2.1.90 | L-rhamnonate | Azotobacter vinelandii | step in the alternative pathway for L-rhamnose metabolism, overview | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | Debaryomyces hansenii | step in the alternative pathway for L-rhamnose metabolism, overview | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | Scheffersomyces stipitis | step in the alternative pathway for L-rhamnose metabolism, overview | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | Debaryomyces hansenii NBRC0083 | step in the alternative pathway for L-rhamnose metabolism, overview | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | Azotobacter vinelandii NBRC 102612 | step in the alternative pathway for L-rhamnose metabolism, overview | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.173 | Debaryomyces hansenii | - |
gene LRA1 | - |
1.1.1.173 | Debaryomyces hansenii NBRC0083 | - |
gene LRA1 | - |
1.1.1.173 | Scheffersomyces stipitis | - |
gene LRA1 | - |
1.1.1.378 | Azotobacter vinelandii | - |
- |
- |
1.1.1.378 | Azotobacter vinelandii NBRC 102612 | - |
- |
- |
3.1.1.65 | Azotobacter vinelandii | - |
- |
- |
3.1.1.65 | Azotobacter vinelandii NBRC 102612 | - |
- |
- |
3.1.1.65 | Debaryomyces hansenii | - |
- |
- |
3.1.1.65 | Debaryomyces hansenii NBRC0083 | - |
- |
- |
3.1.1.65 | Scheffersomyces stipitis | A3LZU8 | - |
- |
4.1.2.53 | Azotobacter vinelandii | - |
- |
- |
4.1.2.53 | Scheffersomyces stipitis | - |
- |
- |
4.2.1.90 | Azotobacter vinelandii | - |
- |
- |
4.2.1.90 | Azotobacter vinelandii NBRC 102612 | - |
- |
- |
4.2.1.90 | Debaryomyces hansenii | - |
- |
- |
4.2.1.90 | Debaryomyces hansenii NBRC0083 | - |
- |
- |
4.2.1.90 | Scheffersomyces stipitis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.173 | native enzyme 42.8fold by anion exchange chromatography, ultrafiltration, gel filtration, hydroxyapatite chromatography, and again gel filtration, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Scheffersomyces stipitis |
1.1.1.173 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Debaryomyces hansenii |
1.1.1.378 | - |
Azotobacter vinelandii |
3.1.1.65 | nickel-nitrilotriacetic acid superflow column | Azotobacter vinelandii |
3.1.1.65 | nickel-nitrilotriacetic acid superflow column | Debaryomyces hansenii |
3.1.1.65 | nickel-nitrilotriacetic acid superflow column | Scheffersomyces stipitis |
4.1.2.53 | Ni-NTA column chromatography | Azotobacter vinelandii |
4.1.2.53 | Ni-NTA column chromatography | Scheffersomyces stipitis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.173 | L-rhamnofuranose + NAD+ = L-rhamno-1,4-lactone + NADH + H+ | the catalytic triad is formed by conserved Ser-Tyr-Lys residues | Debaryomyces hansenii | |
1.1.1.173 | L-rhamnofuranose + NAD+ = L-rhamno-1,4-lactone + NADH + H+ | the catalytic triad is formed by conserved Ser148-Tyr161-Lys165 residues | Scheffersomyces stipitis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.173 | 29.9 | - |
purified native enzyme | Scheffersomyces stipitis |
1.1.1.173 | 50.4 | - |
purified recombinant enzyme | Scheffersomyces stipitis |
1.1.1.173 | 53.3 | - |
purified recombinant enzyme | Debaryomyces hansenii |
1.1.1.378 | 0.15 | - |
pH 9.0, 30°C, substrates: L-fucose, NAD+ | Azotobacter vinelandii |
1.1.1.378 | 0.27 | - |
pH 9.0, 30°C, substrates: L-fucose, NADP+ | Azotobacter vinelandii |
1.1.1.378 | 1.69 | - |
pH 9.0, 30°C, substrates: L-mannose, NAD+ | Azotobacter vinelandii |
1.1.1.378 | 3.64 | - |
pH 9.0, 30°C, substrates: L-mannose, NADP+ | Azotobacter vinelandii |
1.1.1.378 | 50.7 | - |
pH 9.0, 30°C, substrates: L-lyxose, NAD+ | Azotobacter vinelandii |
1.1.1.378 | 72.1 | - |
pH 9.0, 30°C, substrates: L-rhamnose, NAD+ | Azotobacter vinelandii |
1.1.1.378 | 125 | - |
pH 9.0, 30°C, substrates: L-lyxose, NADP+ | Azotobacter vinelandii |
1.1.1.378 | 163 | - |
pH 9.0, 30°C, substrates: L-rhamnose, NADP+ | Azotobacter vinelandii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.173 | L-rhamnose + NAD+ | - |
Debaryomyces hansenii | L-rhamno-1,4-lactone + NADH | - |
r | |
1.1.1.173 | L-rhamnose + NAD+ | - |
Scheffersomyces stipitis | L-rhamno-1,4-lactone + NADH | - |
r | |
1.1.1.173 | L-rhamnose + NAD+ | - |
Debaryomyces hansenii NBRC0083 | L-rhamno-1,4-lactone + NADH | - |
r | |
1.1.1.378 | L-fucose + NAD+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | ? | - |
? | |
1.1.1.378 | L-fucose + NADP+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | ? | - |
? | |
1.1.1.378 | L-lyxose + NAD+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | ? | - |
? | |
1.1.1.378 | L-lyxose + NADP+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | ? | - |
? | |
1.1.1.378 | L-mannose + NAD+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | ? | - |
? | |
1.1.1.378 | L-mannose + NADP+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | ? | - |
? | |
1.1.1.378 | L-rhamnose + NAD(P)+ | first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway | Azotobacter vinelandii | L-rhamno-1,4-lactone + NAD(P)H + H+ | - |
? | |
1.1.1.378 | L-rhamnose + NAD+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | L-rhamno-1,4-lactone + NADH + H+ | - |
? | |
1.1.1.378 | L-rhamnose + NADP+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | L-rhamno-1,4-lactone + NADPH + H+ | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | - |
Azotobacter vinelandii | L-rhamnonate | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | - |
Debaryomyces hansenii | L-rhamnonate | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | - |
Scheffersomyces stipitis | L-rhamnonate | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | - |
Debaryomyces hansenii NBRC0083 | L-rhamnonate | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | - |
Azotobacter vinelandii NBRC 102612 | L-rhamnonate | - |
? | |
4.1.2.53 | 2-dehydro-3-deoxy-L-mannonate | 13.3% activity compared to 2-dehydro-3-deoxy-L-rhamnonate | Scheffersomyces stipitis | ? | - |
? | |
4.1.2.53 | 2-dehydro-3-deoxy-L-mannonate | 6.7% activity compared to 2-dehydro-3-deoxy-L-rhamnonate | Azotobacter vinelandii | ? | - |
? | |
4.1.2.53 | 2-dehydro-3-deoxy-L-rhamnonate | preferred substrate | Azotobacter vinelandii | pyruvate + (R)-lactaldehyde | - |
? | |
4.1.2.53 | 2-dehydro-3-deoxy-L-rhamnonate | preferred substrate | Scheffersomyces stipitis | pyruvate + (R)-lactaldehyde | - |
? | |
4.1.2.53 | 2-dehydro-3-deoxy-L-xylonate | 54.7% activity compared to 2-dehydro-3-deoxy-L-rhamnonate | Scheffersomyces stipitis | ? | - |
? | |
4.1.2.53 | 2-dehydro-3-deoxy-L-xylonate | 7.6% activity compared to 2-dehydro-3-deoxy-L-rhamnonate | Azotobacter vinelandii | ? | - |
? | |
4.2.1.90 | L-lyxonate | - |
Azotobacter vinelandii | 2-dehydro-3-deoxy-L-lyxonate + H2O | - |
? | |
4.2.1.90 | L-lyxonate | - |
Azotobacter vinelandii NBRC 102612 | 2-dehydro-3-deoxy-L-lyxonate + H2O | - |
? | |
4.2.1.90 | L-mannonate | - |
Azotobacter vinelandii | 2-dehydro-3-deoxy-L-mannonate + H2O | - |
? | |
4.2.1.90 | L-mannonate | - |
Azotobacter vinelandii NBRC 102612 | 2-dehydro-3-deoxy-L-mannonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | - |
Debaryomyces hansenii | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | - |
Scheffersomyces stipitis | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | step in the alternative pathway for L-rhamnose metabolism, overview | Azotobacter vinelandii | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | step in the alternative pathway for L-rhamnose metabolism, overview | Debaryomyces hansenii | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | step in the alternative pathway for L-rhamnose metabolism, overview | Scheffersomyces stipitis | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | the catalytic mechanism is similar to those of the known acid-sugar dehydratases in this protein family | Azotobacter vinelandii | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | - |
Debaryomyces hansenii NBRC0083 | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | step in the alternative pathway for L-rhamnose metabolism, overview | Debaryomyces hansenii NBRC0083 | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | step in the alternative pathway for L-rhamnose metabolism, overview | Azotobacter vinelandii NBRC 102612 | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | the catalytic mechanism is similar to those of the known acid-sugar dehydratases in this protein family | Azotobacter vinelandii NBRC 102612 | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.173 | ? | x * 25000, SDS-PAGE | Scheffersomyces stipitis |
4.1.2.53 | ? | x * about 35000, SDS-PAGE | Azotobacter vinelandii |
4.1.2.53 | ? | x * about 35000, SDS-PAGE | Scheffersomyces stipitis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.378 | AvLRA1 | - |
Azotobacter vinelandii |
3.1.1.65 | L-rhamnono-gamma-lactonase | - |
Azotobacter vinelandii |
3.1.1.65 | L-rhamnono-gamma-lactonase | - |
Debaryomyces hansenii |
3.1.1.65 | L-rhamnono-gamma-lactonase | - |
Scheffersomyces stipitis |
3.1.1.65 | LRA2 | - |
Azotobacter vinelandii |
3.1.1.65 | LRA2 | - |
Scheffersomyces stipitis |
4.1.2.53 | L-KDR aldolase | - |
Azotobacter vinelandii |
4.1.2.53 | L-KDR aldolase | - |
Scheffersomyces stipitis |
4.1.2.53 | LRA4 | - |
Azotobacter vinelandii |
4.1.2.53 | LRA4 | - |
Scheffersomyces stipitis |
4.2.1.90 | LRA3 | - |
Azotobacter vinelandii |
4.2.1.90 | LRA3 | - |
Debaryomyces hansenii |
4.2.1.90 | LRA3 | - |
Scheffersomyces stipitis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.173 | 30 | - |
oxidation assay at | Debaryomyces hansenii |
1.1.1.173 | 30 | - |
oxidation assay at | Scheffersomyces stipitis |
1.1.1.378 | 30 | - |
assay at | Azotobacter vinelandii |
4.2.1.90 | 30 | - |
assay at | Azotobacter vinelandii |
4.2.1.90 | 30 | - |
assay at | Debaryomyces hansenii |
4.2.1.90 | 30 | - |
assay at | Scheffersomyces stipitis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.173 | 1510 | - |
L-rhamnose | pH 9.0, 30°C, recombinant enzyme | Scheffersomyces stipitis | |
1.1.1.173 | 2860 | - |
L-rhamnose | pH 9.0, 30°C, recombinant enzyme | Debaryomyces hansenii | |
1.1.1.378 | 4.8 | - |
L-fucose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
1.1.1.378 | 9.2 | - |
L-fucose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
1.1.1.378 | 9.4 | - |
L-Mannose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
1.1.1.378 | 17 | - |
L-Mannose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
1.1.1.378 | 37.2 | - |
L-rhamnose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
1.1.1.378 | 41.3 | - |
L-Lyxose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
1.1.1.378 | 74.8 | - |
L-rhamnose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
1.1.1.378 | 79.7 | - |
L-Lyxose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
4.1.2.53 | 49.3 | - |
2-dehydro-3-deoxy-L-rhamnonate | in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication | Scheffersomyces stipitis | |
4.1.2.53 | 54.7 | - |
2-dehydro-3-deoxy-L-rhamnonate | in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication | Azotobacter vinelandii | |
4.2.1.90 | 0.72 | - |
L-Rhamnonate | pH 7.0, 30°C | Azotobacter vinelandii | |
4.2.1.90 | 49.3 | - |
L-Rhamnonate | pH 7.0, 30°C | Scheffersomyces stipitis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.173 | 9 | - |
oxidation assay at | Debaryomyces hansenii |
1.1.1.173 | 9 | - |
oxidation assay at | Scheffersomyces stipitis |
1.1.1.378 | 9 | - |
assay at | Azotobacter vinelandii |
4.2.1.90 | 7 | - |
assay at | Azotobacter vinelandii |
4.2.1.90 | 7 | - |
assay at | Debaryomyces hansenii |
4.2.1.90 | 7 | - |
assay at | Scheffersomyces stipitis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.173 | NAD+ | strictly dependent on | Debaryomyces hansenii | |
1.1.1.173 | NAD+ | strictly dependent on | Scheffersomyces stipitis | |
1.1.1.173 | NADH | strictly dependent on | Debaryomyces hansenii | |
1.1.1.173 | NADH | strictly dependent on | Scheffersomyces stipitis | |
1.1.1.378 | NAD+ | - |
Azotobacter vinelandii | |
1.1.1.378 | NADP+ | - |
Azotobacter vinelandii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.378 | physiological function | first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway | Azotobacter vinelandii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.378 | 0.007 | - |
L-fucose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
1.1.1.378 | 0.016 | - |
L-fucose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
1.1.1.378 | 0.09 | - |
L-Mannose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
1.1.1.378 | 0.19 | - |
L-Mannose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
1.1.1.378 | 4.9 | - |
L-Lyxose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
1.1.1.378 | 14.3 | - |
L-rhamnose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
1.1.1.378 | 16.05 | - |
L-Lyxose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
1.1.1.378 | 35.7 | - |
L-rhamnose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
4.1.2.53 | 36.2 | - |
2-dehydro-3-deoxy-L-rhamnonate | in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication | Scheffersomyces stipitis | |
4.1.2.53 | 60.2 | - |
2-dehydro-3-deoxy-L-rhamnonate | in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication | Azotobacter vinelandii |