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Literature summary extracted from

  • Watanabe, S.; Saimura, M.; Makino, K.
    Eukaryotic and bacterial gene clusters related to an alternative pathway of non-phosphorylated L-rhamnose metabolism (2008), J. Biol. Chem., 283, 20372-20382.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.173 gene LRA1, DNA and amino acid sequence determination and analysis, genetic organization, functional expression of His-tagged enzyme in Escherichia coli Debaryomyces hansenii
1.1.1.173 gene LRA1, DNA and amino acid sequence determination and analysis, genetic organization, functional expression of His-tagged enzyme in Escherichia coli Scheffersomyces stipitis
1.1.1.378 expression in Escherichia coli Azotobacter vinelandii
3.1.1.65 overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme Azotobacter vinelandii
3.1.1.65 overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme Debaryomyces hansenii
3.1.1.65 overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme Scheffersomyces stipitis
4.1.2.53 expressed in Escherichia coli DH5alpha cells Azotobacter vinelandii
4.1.2.53 expressed in Escherichia coli DH5alpha cells Scheffersomyces stipitis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.53 Ca2+ complete inhibition in the presence of Ca2+ Azotobacter vinelandii
4.1.2.53 Co2+ about 75% residual activity in the presence of Co2+ Azotobacter vinelandii
4.1.2.53 Cu2+ complete inhibition in the presence of Cu2+ Azotobacter vinelandii
4.1.2.53 Fe2+ complete inhibition in the presence of Fe2+ Azotobacter vinelandii
4.1.2.53 Mg2+ about 25% residual activity in the presence of Mg2+ Azotobacter vinelandii
4.1.2.53 Ni2+ about 10% residual activity in the presence of Ni2+ Azotobacter vinelandii
4.1.2.53 Zn2+ about 80% residual activity in the presence of Zn2+ Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.173 1.71
-
L-rhamnose pH 9.0, 30°C, recombinant enzyme Scheffersomyces stipitis
1.1.1.173 9.35
-
L-rhamnose pH 9.0, 30°C, recombinant enzyme Debaryomyces hansenii
1.1.1.378 2.34
-
L-rhamnose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
1.1.1.378 2.61
-
L-rhamnose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
1.1.1.378 4.96
-
L-Lyxose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
1.1.1.378 8.43
-
L-Lyxose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
1.1.1.378 89.3
-
L-Mannose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
1.1.1.378 108
-
L-Mannose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
1.1.1.378 582
-
L-fucose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
1.1.1.378 673
-
L-fucose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
4.1.2.53 0.915
-
2-dehydro-3-deoxy-L-rhamnonate in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication Azotobacter vinelandii
4.1.2.53 1.37
-
2-dehydro-3-deoxy-L-rhamnonate in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication Scheffersomyces stipitis
4.2.1.90 0.115
-
L-Rhamnonate pH 7.0, 30°C Azotobacter vinelandii
4.2.1.90 1.37
-
L-Rhamnonate pH 7.0, 30°C Scheffersomyces stipitis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.53 Co2+ about 240% activity in the presence of Co2+ Scheffersomyces stipitis
4.1.2.53 Mg2+ about 150% activity in the presence of Co2+ Scheffersomyces stipitis
4.1.2.53 Mg2+ more than 250% activity in the presence of Mg2+, 5 mM MgCl2 used in assay conditions Scheffersomyces stipitis
4.1.2.53 additional information not stimulated by Mg2+, Co2+, Ni2+, Zn2+, Fe2+, Cu2+, and Ca2+ Azotobacter vinelandii
4.1.2.53 additional information not stimulated by Zn2+, Fe2+, Cu2+, and Ca2+ Scheffersomyces stipitis
4.1.2.53 Ni2+ about 140% activity in the presence of Ni2+ Scheffersomyces stipitis
4.2.1.90 Mg2+
-
Azotobacter vinelandii
4.2.1.90 Mg2+
-
Debaryomyces hansenii
4.2.1.90 Mg2+
-
Scheffersomyces stipitis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.173 25000
-
x * 25000, SDS-PAGE Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.173 L-rhamnose + NAD+ Debaryomyces hansenii
-
L-rhamno-1,4-lactone + NADH
-
r
1.1.1.173 L-rhamnose + NAD+ Scheffersomyces stipitis
-
L-rhamno-1,4-lactone + NADH
-
r
1.1.1.173 L-rhamnose + NAD+ Debaryomyces hansenii NBRC0083
-
L-rhamno-1,4-lactone + NADH
-
r
1.1.1.378 L-rhamnose + NAD(P)+ Azotobacter vinelandii first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway L-rhamno-1,4-lactone + NAD(P)H + H+
-
?
3.1.1.65 L-rhamnono-1,4-lactone + H2O Azotobacter vinelandii
-
L-rhamnonate
-
?
3.1.1.65 L-rhamnono-1,4-lactone + H2O Debaryomyces hansenii
-
L-rhamnonate
-
?
3.1.1.65 L-rhamnono-1,4-lactone + H2O Scheffersomyces stipitis
-
L-rhamnonate
-
?
3.1.1.65 L-rhamnono-1,4-lactone + H2O Debaryomyces hansenii NBRC0083
-
L-rhamnonate
-
?
3.1.1.65 L-rhamnono-1,4-lactone + H2O Azotobacter vinelandii NBRC 102612
-
L-rhamnonate
-
?
4.1.2.53 2-dehydro-3-deoxy-L-rhamnonate Azotobacter vinelandii preferred substrate pyruvate + (R)-lactaldehyde
-
?
4.1.2.53 2-dehydro-3-deoxy-L-rhamnonate Scheffersomyces stipitis preferred substrate pyruvate + (R)-lactaldehyde
-
?
4.2.1.90 L-rhamnonate Azotobacter vinelandii step in the alternative pathway for L-rhamnose metabolism, overview 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate Debaryomyces hansenii step in the alternative pathway for L-rhamnose metabolism, overview 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate Scheffersomyces stipitis step in the alternative pathway for L-rhamnose metabolism, overview 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate Debaryomyces hansenii NBRC0083 step in the alternative pathway for L-rhamnose metabolism, overview 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate Azotobacter vinelandii NBRC 102612 step in the alternative pathway for L-rhamnose metabolism, overview 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.173 Debaryomyces hansenii
-
gene LRA1
-
1.1.1.173 Debaryomyces hansenii NBRC0083
-
gene LRA1
-
1.1.1.173 Scheffersomyces stipitis
-
gene LRA1
-
1.1.1.378 Azotobacter vinelandii
-
-
-
1.1.1.378 Azotobacter vinelandii NBRC 102612
-
-
-
3.1.1.65 Azotobacter vinelandii
-
-
-
3.1.1.65 Azotobacter vinelandii NBRC 102612
-
-
-
3.1.1.65 Debaryomyces hansenii
-
-
-
3.1.1.65 Debaryomyces hansenii NBRC0083
-
-
-
3.1.1.65 Scheffersomyces stipitis A3LZU8
-
-
4.1.2.53 Azotobacter vinelandii
-
-
-
4.1.2.53 Scheffersomyces stipitis
-
-
-
4.2.1.90 Azotobacter vinelandii
-
-
-
4.2.1.90 Azotobacter vinelandii NBRC 102612
-
-
-
4.2.1.90 Debaryomyces hansenii
-
-
-
4.2.1.90 Debaryomyces hansenii NBRC0083
-
-
-
4.2.1.90 Scheffersomyces stipitis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.173 native enzyme 42.8fold by anion exchange chromatography, ultrafiltration, gel filtration, hydroxyapatite chromatography, and again gel filtration, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Scheffersomyces stipitis
1.1.1.173 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Debaryomyces hansenii
1.1.1.378
-
Azotobacter vinelandii
3.1.1.65 nickel-nitrilotriacetic acid superflow column Azotobacter vinelandii
3.1.1.65 nickel-nitrilotriacetic acid superflow column Debaryomyces hansenii
3.1.1.65 nickel-nitrilotriacetic acid superflow column Scheffersomyces stipitis
4.1.2.53 Ni-NTA column chromatography Azotobacter vinelandii
4.1.2.53 Ni-NTA column chromatography Scheffersomyces stipitis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.173 L-rhamnofuranose + NAD+ = L-rhamno-1,4-lactone + NADH + H+ the catalytic triad is formed by conserved Ser-Tyr-Lys residues Debaryomyces hansenii
1.1.1.173 L-rhamnofuranose + NAD+ = L-rhamno-1,4-lactone + NADH + H+ the catalytic triad is formed by conserved Ser148-Tyr161-Lys165 residues Scheffersomyces stipitis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.173 29.9
-
purified native enzyme Scheffersomyces stipitis
1.1.1.173 50.4
-
purified recombinant enzyme Scheffersomyces stipitis
1.1.1.173 53.3
-
purified recombinant enzyme Debaryomyces hansenii
1.1.1.378 0.15
-
pH 9.0, 30°C, substrates: L-fucose, NAD+ Azotobacter vinelandii
1.1.1.378 0.27
-
pH 9.0, 30°C, substrates: L-fucose, NADP+ Azotobacter vinelandii
1.1.1.378 1.69
-
pH 9.0, 30°C, substrates: L-mannose, NAD+ Azotobacter vinelandii
1.1.1.378 3.64
-
pH 9.0, 30°C, substrates: L-mannose, NADP+ Azotobacter vinelandii
1.1.1.378 50.7
-
pH 9.0, 30°C, substrates: L-lyxose, NAD+ Azotobacter vinelandii
1.1.1.378 72.1
-
pH 9.0, 30°C, substrates: L-rhamnose, NAD+ Azotobacter vinelandii
1.1.1.378 125
-
pH 9.0, 30°C, substrates: L-lyxose, NADP+ Azotobacter vinelandii
1.1.1.378 163
-
pH 9.0, 30°C, substrates: L-rhamnose, NADP+ Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.173 L-rhamnose + NAD+
-
Debaryomyces hansenii L-rhamno-1,4-lactone + NADH
-
r
1.1.1.173 L-rhamnose + NAD+
-
Scheffersomyces stipitis L-rhamno-1,4-lactone + NADH
-
r
1.1.1.173 L-rhamnose + NAD+
-
Debaryomyces hansenii NBRC0083 L-rhamno-1,4-lactone + NADH
-
r
1.1.1.378 L-fucose + NAD+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii ?
-
?
1.1.1.378 L-fucose + NADP+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii ?
-
?
1.1.1.378 L-lyxose + NAD+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii ?
-
?
1.1.1.378 L-lyxose + NADP+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii ?
-
?
1.1.1.378 L-mannose + NAD+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii ?
-
?
1.1.1.378 L-mannose + NADP+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii ?
-
?
1.1.1.378 L-rhamnose + NAD(P)+ first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway Azotobacter vinelandii L-rhamno-1,4-lactone + NAD(P)H + H+
-
?
1.1.1.378 L-rhamnose + NAD+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii L-rhamno-1,4-lactone + NADH + H+
-
?
1.1.1.378 L-rhamnose + NADP+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii L-rhamno-1,4-lactone + NADPH + H+
-
?
3.1.1.65 L-rhamnono-1,4-lactone + H2O
-
Azotobacter vinelandii L-rhamnonate
-
?
3.1.1.65 L-rhamnono-1,4-lactone + H2O
-
Debaryomyces hansenii L-rhamnonate
-
?
3.1.1.65 L-rhamnono-1,4-lactone + H2O
-
Scheffersomyces stipitis L-rhamnonate
-
?
3.1.1.65 L-rhamnono-1,4-lactone + H2O
-
Debaryomyces hansenii NBRC0083 L-rhamnonate
-
?
3.1.1.65 L-rhamnono-1,4-lactone + H2O
-
Azotobacter vinelandii NBRC 102612 L-rhamnonate
-
?
4.1.2.53 2-dehydro-3-deoxy-L-mannonate 13.3% activity compared to 2-dehydro-3-deoxy-L-rhamnonate Scheffersomyces stipitis ?
-
?
4.1.2.53 2-dehydro-3-deoxy-L-mannonate 6.7% activity compared to 2-dehydro-3-deoxy-L-rhamnonate Azotobacter vinelandii ?
-
?
4.1.2.53 2-dehydro-3-deoxy-L-rhamnonate preferred substrate Azotobacter vinelandii pyruvate + (R)-lactaldehyde
-
?
4.1.2.53 2-dehydro-3-deoxy-L-rhamnonate preferred substrate Scheffersomyces stipitis pyruvate + (R)-lactaldehyde
-
?
4.1.2.53 2-dehydro-3-deoxy-L-xylonate 54.7% activity compared to 2-dehydro-3-deoxy-L-rhamnonate Scheffersomyces stipitis ?
-
?
4.1.2.53 2-dehydro-3-deoxy-L-xylonate 7.6% activity compared to 2-dehydro-3-deoxy-L-rhamnonate Azotobacter vinelandii ?
-
?
4.2.1.90 L-lyxonate
-
Azotobacter vinelandii 2-dehydro-3-deoxy-L-lyxonate + H2O
-
?
4.2.1.90 L-lyxonate
-
Azotobacter vinelandii NBRC 102612 2-dehydro-3-deoxy-L-lyxonate + H2O
-
?
4.2.1.90 L-mannonate
-
Azotobacter vinelandii 2-dehydro-3-deoxy-L-mannonate + H2O
-
?
4.2.1.90 L-mannonate
-
Azotobacter vinelandii NBRC 102612 2-dehydro-3-deoxy-L-mannonate + H2O
-
?
4.2.1.90 L-rhamnonate
-
Debaryomyces hansenii 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate
-
Scheffersomyces stipitis 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate step in the alternative pathway for L-rhamnose metabolism, overview Azotobacter vinelandii 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate step in the alternative pathway for L-rhamnose metabolism, overview Debaryomyces hansenii 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate step in the alternative pathway for L-rhamnose metabolism, overview Scheffersomyces stipitis 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate the catalytic mechanism is similar to those of the known acid-sugar dehydratases in this protein family Azotobacter vinelandii 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate
-
Debaryomyces hansenii NBRC0083 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate step in the alternative pathway for L-rhamnose metabolism, overview Debaryomyces hansenii NBRC0083 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate step in the alternative pathway for L-rhamnose metabolism, overview Azotobacter vinelandii NBRC 102612 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?
4.2.1.90 L-rhamnonate the catalytic mechanism is similar to those of the known acid-sugar dehydratases in this protein family Azotobacter vinelandii NBRC 102612 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.173 ? x * 25000, SDS-PAGE Scheffersomyces stipitis
4.1.2.53 ? x * about 35000, SDS-PAGE Azotobacter vinelandii
4.1.2.53 ? x * about 35000, SDS-PAGE Scheffersomyces stipitis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.378 AvLRA1
-
Azotobacter vinelandii
3.1.1.65 L-rhamnono-gamma-lactonase
-
Azotobacter vinelandii
3.1.1.65 L-rhamnono-gamma-lactonase
-
Debaryomyces hansenii
3.1.1.65 L-rhamnono-gamma-lactonase
-
Scheffersomyces stipitis
3.1.1.65 LRA2
-
Azotobacter vinelandii
3.1.1.65 LRA2
-
Scheffersomyces stipitis
4.1.2.53 L-KDR aldolase
-
Azotobacter vinelandii
4.1.2.53 L-KDR aldolase
-
Scheffersomyces stipitis
4.1.2.53 LRA4
-
Azotobacter vinelandii
4.1.2.53 LRA4
-
Scheffersomyces stipitis
4.2.1.90 LRA3
-
Azotobacter vinelandii
4.2.1.90 LRA3
-
Debaryomyces hansenii
4.2.1.90 LRA3
-
Scheffersomyces stipitis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.173 30
-
oxidation assay at Debaryomyces hansenii
1.1.1.173 30
-
oxidation assay at Scheffersomyces stipitis
1.1.1.378 30
-
assay at Azotobacter vinelandii
4.2.1.90 30
-
assay at Azotobacter vinelandii
4.2.1.90 30
-
assay at Debaryomyces hansenii
4.2.1.90 30
-
assay at Scheffersomyces stipitis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.173 1510
-
L-rhamnose pH 9.0, 30°C, recombinant enzyme Scheffersomyces stipitis
1.1.1.173 2860
-
L-rhamnose pH 9.0, 30°C, recombinant enzyme Debaryomyces hansenii
1.1.1.378 4.8
-
L-fucose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
1.1.1.378 9.2
-
L-fucose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
1.1.1.378 9.4
-
L-Mannose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
1.1.1.378 17
-
L-Mannose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
1.1.1.378 37.2
-
L-rhamnose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
1.1.1.378 41.3
-
L-Lyxose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
1.1.1.378 74.8
-
L-rhamnose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
1.1.1.378 79.7
-
L-Lyxose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
4.1.2.53 49.3
-
2-dehydro-3-deoxy-L-rhamnonate in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication Scheffersomyces stipitis
4.1.2.53 54.7
-
2-dehydro-3-deoxy-L-rhamnonate in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication Azotobacter vinelandii
4.2.1.90 0.72
-
L-Rhamnonate pH 7.0, 30°C Azotobacter vinelandii
4.2.1.90 49.3
-
L-Rhamnonate pH 7.0, 30°C Scheffersomyces stipitis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.173 9
-
oxidation assay at Debaryomyces hansenii
1.1.1.173 9
-
oxidation assay at Scheffersomyces stipitis
1.1.1.378 9
-
assay at Azotobacter vinelandii
4.2.1.90 7
-
assay at Azotobacter vinelandii
4.2.1.90 7
-
assay at Debaryomyces hansenii
4.2.1.90 7
-
assay at Scheffersomyces stipitis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.173 NAD+ strictly dependent on Debaryomyces hansenii
1.1.1.173 NAD+ strictly dependent on Scheffersomyces stipitis
1.1.1.173 NADH strictly dependent on Debaryomyces hansenii
1.1.1.173 NADH strictly dependent on Scheffersomyces stipitis
1.1.1.378 NAD+
-
Azotobacter vinelandii
1.1.1.378 NADP+
-
Azotobacter vinelandii

General Information

EC Number General Information Comment Organism
1.1.1.378 physiological function first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway Azotobacter vinelandii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.378 0.007
-
L-fucose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
1.1.1.378 0.016
-
L-fucose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
1.1.1.378 0.09
-
L-Mannose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
1.1.1.378 0.19
-
L-Mannose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
1.1.1.378 4.9
-
L-Lyxose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
1.1.1.378 14.3
-
L-rhamnose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
1.1.1.378 16.05
-
L-Lyxose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
1.1.1.378 35.7
-
L-rhamnose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
4.1.2.53 36.2
-
2-dehydro-3-deoxy-L-rhamnonate in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication Scheffersomyces stipitis
4.1.2.53 60.2
-
2-dehydro-3-deoxy-L-rhamnonate in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication Azotobacter vinelandii