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Literature summary extracted from

  • Han, Y.; Joosten, H.J.; Niu, W.; Zhao, Z.; Mariano, P.S.; McCalman, M.; van Kan, J.; Schaap, P.J.; Dunaway-Mariano, D.
    Oxaloacetate hydrolase, the C-C bond lyase of oxalate secreting fungi (2007), J. Biol. Chem., 282, 9581-9590.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.7.1.1 food industry oxalate toxicity is a concern for the commercial application of fungi in the food and drug industries Botrytis cinerea
3.7.1.1 food industry oxalate toxicity is a concern for the commercial application of fungi in the food and drug industries Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.1 into the vector pGEM-T Easy for sequencing Aspergillus niger
3.7.1.1 into the vector pGEM-T Easy for sequencing, and into pET-3c for expression in Escherichia coli BL21DE3 cells Botrytis cinerea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.1 3,3-difluorooxaloacetate
-
 Aspergillus niger
3.7.1.1 3,3-difluorooxaloacetate
-
 Botrytis cinerea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.1 0.062
-
 oxaloacetate cofactor Mg2+, buffer imidazole Botrytis cinerea
3.7.1.1 0.064
-
 oxaloacetate cofactor Mg2+, buffer Hepes Botrytis cinerea
3.7.1.1 0.084
-
 oxaloacetate cofactor Mg2+, buffer Tris Botrytis cinerea
3.7.1.1 0.24
-
 oxaloacetate cofactor Mn2+, buffer imidazole Botrytis cinerea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.1 34360
-
 theoretical molecular mass Botrytis cinerea
3.7.1.1 35000
-
 determined by SDS-PAGE Botrytis cinerea
3.7.1.1 100000
-
 determined by gel filtration, indicative of a homotrimeric quaternary structure Botrytis cinerea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.1 oxaloacetate + H2O Botrytis cinerea
-
 oxalate + acetate
-
 ?
3.7.1.1 oxaloacetate + H2O Aspergillus niger
-
 oxalate + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.1 Aspergillus niger Q7Z986
-
-
3.7.1.1 Botrytis cinerea Q6PNM8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.1 by ammonium sulfate-induced protein precipitation, and by using of a DEAE-cellulose and a butyl-Sepharose column Botrytis cinerea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.1 0.62
-
 cell extract Botrytis cinerea
3.7.1.1 4.6
-
 purification step ammonium sulfate precipitation Botrytis cinerea
3.7.1.1 15
-
 purification step DEAE-cellulose column Botrytis cinerea
3.7.1.1 20
-
 purification step butyl-Sepharose column Botrytis cinerea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.1 oxaloacetate + H2O
-
 Botrytis cinerea oxalate + acetate
-
 ?
3.7.1.1 oxaloacetate + H2O
-
 Aspergillus niger oxalate + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.1 More the results are consistent with the existence of a monomer-dimer-tetramer equilibrium Botrytis cinerea

Synonyms

EC Number Synonyms Comment Organism
3.7.1.1 OAH
-
 Botrytis cinerea
3.7.1.1 OAH
-
 Aspergillus niger
3.7.1.1 oxaloacetate acetylhydrolase
-
 Botrytis cinerea
3.7.1.1 oxaloacetate acetylhydrolase
-
 Aspergillus niger
3.7.1.1 oxaloacetate hydrolase
-
 Botrytis cinerea
3.7.1.1 oxaloacetate hydrolase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.7.1.1 25
-
 oxaloacetate hydrolase assay Botrytis cinerea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.1 0.35
-
 oxaloacetate cofactor Ca2+, buffer imidazole Botrytis cinerea
3.7.1.1 9.5
-
 oxaloacetate cofactor Mg2+, buffer Tris Botrytis cinerea
3.7.1.1 9.6
-
 oxaloacetate cofactor Mg2+, buffer Hepes Botrytis cinerea
3.7.1.1 10.3
-
 oxaloacetate cofactor Mg2+, buffer imidazole Botrytis cinerea
3.7.1.1 11.8
-
 oxaloacetate cofactor Mg2+, buffer imidazole Botrytis cinerea
3.7.1.1 12.5
-
 oxaloacetate cofactor Mg2+, buffer Hepes Botrytis cinerea
3.7.1.1 32
-
 oxaloacetate cofactor Mn2+, buffer Hepes Botrytis cinerea
3.7.1.1 37
-
 oxaloacetate cofactor Mn2+, buffer imidazole Botrytis cinerea
3.7.1.1 50
-
 oxaloacetate cofactor Mn2+, buffer imidazole Botrytis cinerea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.1 7.6
-
 oxaloacetate hydrolase assay Botrytis cinerea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.7.1.1 0.000068
-
 3,3-difluorooxaloacetate
-
 Botrytis cinerea