EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.3 | M1A/M16N/M20L/M42Y/C85A/M92F/C152S | mutant ANLYF carries seven amino acid substitutions that result in a methionine- and cysteine-free mutant enzyme with decreased stability. Mutant ANLYF show very high activity and may be stabilized by backbone cyclization via a cyanocysteine-mediated intramolecular ligation reaction, without loss of its high activity | Escherichia coli |
1.5.1.3 | additional information | construction of mutants based on mutant M1A/M16N/M20L/M42Y/C85A/M92F/C152S, i.e. ANLYF. Linear ANLYF-Gn is ANLYF with the N- and C-terminal extensions of Ala-(Gly)n-3 and -(Gly)2-Cys-Ala residues, respectively, with n=6-8, and circular ANLYF-Gn is ANLYF with the C and N termini cross-linked by the linker of the -Gly-Gly-Ala-(Gly)n-3 sequence, n=6-8 | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.0012 | - |
7,8-dihydrofolate | wild-type, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 0.0062 | - |
7,8-dihydrofolate | mutant linear ANLYF-G6, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 0.0083 | - |
7,8-dihydrofolate | mutant M1A/M16N/M20L/M42Y/C85A/M92F/C152S, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 0.0086 | - |
7,8-dihydrofolate | mutant linear ANLYF-G8, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 0.0115 | - |
7,8-dihydrofolate | mutant circular ANLYF-G6, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 0.013 | - |
7,8-dihydrofolate | mutant circular ANLYF-G7, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 0.014 | - |
7,8-dihydrofolate | mutant circular ANLYF-G8, 15°C, pH 7.0 | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Escherichia coli | P0ABQ4 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 40.9 | - |
melting temperature, mutant linear ANLYF-G7 | Escherichia coli |
1.5.1.3 | 42 | - |
melting temperature, mutant M1A/M16N/M20L/M42Y/C85A/M92F/C152S | Escherichia coli |
1.5.1.3 | 44.3 | - |
melting temperature, mutant linear ANLYF-G8 | Escherichia coli |
1.5.1.3 | 44.5 | - |
melting temperature, mutant linear ANLYF-G6 | Escherichia coli |
1.5.1.3 | 49.3 | - |
melting temperature, mutant circular ANLYF-G8 | Escherichia coli |
1.5.1.3 | 49.5 | - |
melting temperature, mutant circular ANLYF-G6 | Escherichia coli |
1.5.1.3 | 50.2 | - |
melting temperature, wild-type | Escherichia coli |
1.5.1.3 | 51.3 | - |
melting temperature, mutant circular ANLYF-G7 | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 5.3 | - |
7,8-dihydrofolate | wild-type, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 34.8 | - |
7,8-dihydrofolate | mutant circular ANLYF-G8, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 36.7 | - |
7,8-dihydrofolate | mutant linear ANLYF-G6, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 38.7 | - |
7,8-dihydrofolate | mutant circular ANLYF-G7, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 40.5 | - |
7,8-dihydrofolate | mutant M1A/M16N/M20L/M42Y/C85A/M92F/C152S, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 40.8 | - |
7,8-dihydrofolate | mutant linear ANLYF-G8, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 41.7 | - |
7,8-dihydrofolate | mutant linear ANLYF-G7, 15°C, pH 7.0 | Escherichia coli | |
1.5.1.3 | 43.7 | - |
7,8-dihydrofolate | mutant circular ANLYF-G6, 15°C, pH 7.0 | Escherichia coli |