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Literature summary extracted from

  • Lee, C.; Kramer, G.; Graham, D.E.; Appling, D.R.
    Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase (2007), J. Biol. Chem., 282, 27744-27753.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.228 expression in Escherichia coli as a fusion protein with maltose-binding protein Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.228 cytoplasm
-
 Saccharomyces cerevisiae 5737
-
2.1.1.228 mitochondrial matrix
-
 Saccharomyces cerevisiae 5759
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Saccharomyces cerevisiae Trm5p is responsible for m1G37 methylation of mitochondrial and cytoplasmic tRNAs S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.228 Saccharomyces cerevisiae P38793
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.228 Trm5p is purified as a fusion protein with maltose-binding protein Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Trm5p is responsible for m1G37 methylation of mitochondrial and cytoplasmic tRNAs Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA methyltransferase activity with tRNA isolated from a DELTAtrm5 mutant strain, as well as with a synthetic mitochondrial initiator tRNA (tRNAMetf). N1-Methylguanine is determined by high pressure liquid chromatography analysis Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA the site of methylation is guanosine 37 in both mitochondrial tRNAMetf and tRNAPhe, determined by primer extension ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.228 Trm5p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.228 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.228 8
-
 assay at Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.1.1.228 malfunction efficiency or accuracy of mitochondrial protein synthesis is decreased in cells lacking m1G37 methylation of mitochondrial tRNAs Saccharomyces cerevisiae
2.1.1.228 physiological function the m1G37 tRNA modification plays an important role in reading frame maintenance in mitochondrial protein synthesis Saccharomyces cerevisiae