EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.88 | diethyldicarbonate | 1 mM, 114% of initial activity | Pseudomonas fluorescens | |
4.1.1.88 | N-Acetylimidazole | 1 mM, 117% of initial activity | Pseudomonas fluorescens | |
4.1.1.88 | pyridoxal 5'phosphate | 1 mM, 112% of initial activity | Pseudomonas fluorescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.88 | 5,5'-dithio-bis(2-nitrobenzoic acid) | 0.5 mM, 1.5% residual activity | Acinetobacter calcoaceticus | |
4.1.1.88 | Bromoacetate | 0.5 mM, 0.9% residual activity | Acinetobacter calcoaceticus | |
4.1.1.88 | Bromoacetate | 0.5 mM, 0.1% residual activity; 0.5 mM, 0.4% residual activity | Pseudomonas fluorescens | |
4.1.1.88 | diethyldicarbonate | 1 mM, 91% residual activity | Acinetobacter calcoaceticus | |
4.1.1.88 | diethyldicarbonate | 1 mM, 88% residual activity | Pseudomonas fluorescens | |
4.1.1.88 | iodoacetamide | 0.5 mM, 58% residual activity | Acinetobacter calcoaceticus | |
4.1.1.88 | N-Acetylimidazole | 1 mM, 95% residual activity | Acinetobacter calcoaceticus | |
4.1.1.88 | N-Acetylimidazole | 1 mM, 95% residual activity | Pseudomonas fluorescens | |
4.1.1.88 | N-ethylmaleimide | 0.5 mM, 0.1% residual activity | Acinetobacter calcoaceticus | |
4.1.1.88 | N-ethylmaleimide | 0.5 mM, 0.2% residual activity | Pseudomonas fluorescens | |
4.1.1.88 | pyridoxal 5'phosphate | 1 mM, 82% residual activity | Acinetobacter calcoaceticus | |
4.1.1.88 | pyridoxal 5'phosphate | 1 mM, 92% residual activity | Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.88 | 11000 | - |
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit | Acinetobacter calcoaceticus |
4.1.1.88 | 11000 | - |
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit | Pseudomonas fluorescens |
4.1.1.88 | 25000 | - |
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit | Acinetobacter calcoaceticus |
4.1.1.88 | 30000 | - |
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit | Pseudomonas fluorescens |
4.1.1.88 | 32000 | - |
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit | Acinetobacter calcoaceticus |
4.1.1.88 | 33000 | - |
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit | Pseudomonas fluorescens |
4.1.1.88 | 65000 | - |
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit | Acinetobacter calcoaceticus |
4.1.1.88 | 65000 | - |
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit | Pseudomonas fluorescens |
4.1.1.88 | 185000 | - |
gel filtration | Pseudomonas fluorescens |
4.1.1.88 | 185000 | - |
gel filtration | Acinetobacter calcoaceticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.88 | Acinetobacter calcoaceticus | - |
grown on malonate | - |
4.1.1.88 | Pseudomonas fluorescens | - |
grown on malonate | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
4.1.1.88 | additional information | the delta subunit of the active form of enzyme is acetylated, the acetyl group may form a thioester bond with the thiol group of the prosthetic group | Pseudomonas fluorescens |
4.1.1.88 | additional information | the delta subunit of the active form of enzyme is acetylated, the acetyl group may form a thioester bond with the thiol group of the prosthetic group | Acinetobacter calcoaceticus |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.88 | - |
Pseudomonas fluorescens |
4.1.1.88 | - |
Acinetobacter calcoaceticus |
EC Number | Renatured (Comment) | Organism |
---|---|---|
4.1.1.88 | reactivation of deacetyl malonate decarboxylase with malonyl-CoA to 100%, with acetyl-CoA or methylmalonyl-CoA to about 50% of initial activity, respectively | Pseudomonas fluorescens |
4.1.1.88 | reactivation of deacetyl malonate decarboxylase with malonyl-CoA, acetyl-CoA or methylmalonyl-CoA to more than 80% of initial activity | Pseudomonas fluorescens |
4.1.1.88 | reactivation of deacetyl malonate decarboxylase with malonyl-CoA, acetyl-CoA or methylmalonyl-CoA to more than 80% of initial activity | Acinetobacter calcoaceticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.88 | 4.48 | - |
30°C | Pseudomonas fluorescens |
4.1.1.88 | 10.02 | - |
30°C | Acinetobacter calcoaceticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.88 | malonate + H+ | - |
Pseudomonas fluorescens | acetate + CO2 | - |
? | |
4.1.1.88 | malonate + H+ | - |
Acinetobacter calcoaceticus | acetate + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.88 | multimer | 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit | Acinetobacter calcoaceticus |
4.1.1.88 | multimer | 2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit | Pseudomonas fluorescens |