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Literature summary extracted from

  • Grosse, S.; Voigt, C.; Wendlandt, K.D.; Kleber, H.P.
    Purification and properties of methanol dehydrogenase from Methylosinus sp. WI 14 (1998), J. Basic Microbiol., 38, 189-196.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.2.7 methylamine can replace NH4+ Methylosinus sp.
1.1.2.7 NH4+ absolute requirement, NH4+ can be replaced by methylamine but not by di- or triamines Methylosinus sp.
1.2.2.B2 methylamine can replace NH4+ Methylosinus sp.
1.2.2.B2 NH4+ absolute requirement, NH4+ can be replaced by methylamine but not by di- or triamines Methylosinus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.7 Cu2+ 38% inhibition at 0.1 mM Methylosinus sp.
1.1.2.7 Fe2+ complete inhibition at 1 mM Methylosinus sp.
1.1.2.7 additional information no or poor inhibition by Ca2+, Co2+, Li+, Mg2+, Mn2+, and KCN, EDTA, and DTE Methylosinus sp.
1.1.2.7 phenazine methosulfate 39% inhibition at 3 mM Methylosinus sp.
1.2.2.B2 Cu2+ 38% inhibition at 0.1 mM Methylosinus sp.
1.2.2.B2 Fe2+ complete inhibition at 1 mM Methylosinus sp.
1.2.2.B2 additional information no or poor inhibition by Ca2+, Co2+, Li+, Mg2+, Mn2+, and KCN, EDTA, and DTE Methylosinus sp.
1.2.2.B2 phenazine methosulfate 39% inhibition at 3 mM Methylosinus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.7 0.18
-
 sorbic alcohol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
1.1.2.7 0.45
-
 methanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
1.1.2.7 0.58
-
 Heptanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
1.1.2.7 0.65
-
 Octanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
1.1.2.7 0.79
-
 Hexanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
1.1.2.7 1.34
-
 Pentanol pH 9.0, 57°C, acceptor 2,6-dichlorophenolindophenol Methylosinus sp.
1.1.2.7 3.58
-
 ethanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
1.1.2.7 3.69
-
 Propanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
1.1.2.7 6.38
-
 butanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.7 soluble
-
 Methylosinus sp.
-
-
1.2.2.B2 soluble
-
 Methylosinus sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.7 70000
-
 2 * 70000, SDS-PAGE Methylosinus sp.
1.1.2.7 140000
-
 gel filtration Methylosinus sp.
1.2.2.B2 70000
-
 2 * 70000, SDS-PAGE Methylosinus sp.
1.2.2.B2 140000
-
 gel filtration Methylosinus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.7 methanol + ferricytochrome cL Methylosinus sp.
-
 formaldehyde + ferrocytochrome cL
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.7 Methylosinus sp.
-
-
-
1.1.2.7 Methylosinus sp. WI 14
-
-
-
1.2.2.B2 Methylosinus sp.
-
-
-
1.2.2.B2 Methylosinus sp. WI 14
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.7 native enzyme 9fold to homogeneity by anion exchange chromatography, ammonium sulfate fractionation, and hydrophobic interaction chromatography, followed by ultrafiltration and gel filtration Methylosinus sp.
1.2.2.B2 native enzyme 9fold to homogeneity by anion exchange chromatography, ammonium sulfate fractionation, and hydrophobic interaction chromatography, followed by ultrafiltration and gel filtration Methylosinus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.7 6.12
-
 purified enzyme Methylosinus sp.
1.2.2.B2 6.12
-
 purified enzyme Methylosinus sp.

Storage Stability

EC Number Storage Stability Organism
1.1.2.7 -18°C, purified native enzyme, 0.05 M sodium phosphate buffer, pH 7.5, loss of 10% of activity after 1 month Methylosinus sp.
1.1.2.7 4°C, purified native enzyme, 0.05 M sodium phosphate buffer, pH 7.5, loss of 30% of activity after 24 h Methylosinus sp.
1.2.2.B2 -18°C, purified native enzyme, 0.05 M sodium phosphate buffer, pH 7.5, loss of 10% of activity after 1 month Methylosinus sp.
1.2.2.B2 4°C, purified native enzyme, 0.05 M sodium phosphate buffer, pH 7.5, loss of 30% of activity after 24 h Methylosinus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.7 butanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. butyraldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.7 ethanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. acetaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.7 heptanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. heptaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.7 hexanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. hexaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.7 methanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.7 methanol + ferricytochrome cL
-
 Methylosinus sp. formaldehyde + ferrocytochrome cL
-
 ?
1.1.2.7 additional information broad substrate specificity, MDH from Methylosinus sp. strain WI 14 oxidises only primary alcohols up to octanol and several aldehydes Methylosinus sp. ?
-
 ?
1.1.2.7 additional information broad substrate specificity, MDH from Methylosinus sp. strain WI 14 oxidises only primary alcohols up to octanol and several aldehydes Methylosinus sp. WI 14 ?
-
 ?
1.1.2.7 octanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. octaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.7 pentanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. pentaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.7 propanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. propionaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.7 sorbic alcohol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.2.B2 acetaldehyde + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. acetate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.2.B2 acetaldehyde + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. WI 14 acetate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.2.B2 formaldehyde + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. formate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.2.B2 formaldehyde + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. WI 14 formate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.2.B2 additional information broad substrate specificity, MDH from Methylosinus sp. strain WI 14 oxidises only primary alcohols up to octanol and several aldehydes Methylosinus sp. ?
-
 ?
1.2.2.B2 additional information broad substrate specificity, MDH from Methylosinus sp. strain WI 14 oxidises only primary alcohols up to octanol and several aldehydes Methylosinus sp. WI 14 ?
-
 ?
1.2.2.B2 propionaldehyde + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. propionate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.2.B2 propionaldehyde + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. WI 14 propionate + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.7 dimer 2 * 70000, SDS-PAGE Methylosinus sp.
1.2.2.B2 dimer 2 * 70000, SDS-PAGE Methylosinus sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.2.7 MDH
-
 Methylosinus sp.
1.1.2.7 methanol dehydrogenase
-
 Methylosinus sp.
1.1.5.5 MDH
-
-
1.1.5.5 methanol dehydrogenase
-
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.7 57
-
-
 Methylosinus sp.
1.2.2.B2 57
-
-
 Methylosinus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.2.B2 25 60
-
 Methylosinus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.2.7 60
-
 30 min, purified enzyme, loss of 25% activity Methylosinus sp.
1.2.2.B2 60
-
 30 min, purified enzyme, loss of 25% activity Methylosinus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.7 9
-
-
 Methylosinus sp.
1.2.2.B2 9
-
-
 Methylosinus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.2.B2 7 10
-
 Methylosinus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.7 pyrroloquinoline quinone prosthetic group, 1.3 molecules per enzyme molecule Methylosinus sp.
1.2.2.B2 pyrroloquinoline quinone prosthetic group, 1.3 molecules per enzyme molecule Methylosinus sp.