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Literature summary extracted from

  • Li, F.; Hinderberger, J.; Seedorf, H.; Zhang, J.; Buckel, W.; Thauer, R.K.
    Coupled ferredoxin and crotonyl coenzyme A (CoA) reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/Etf complex from Clostridium kluyveri (2008), J. Bacteriol., 190, 843-850.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.3.1.109 FMN can not substitute for FAD in stabilizing the enzyme activity Clostridium kluyveri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.109 cytoplasm
-
 Clostridium kluyveri 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.109 28000
-
 x * 41000 + x * 36000 + x * 28000, during purification the butanoyl-CoA dehydrogenase/Etf complex partially dissociated into its components, SDS-PAGE Clostridium kluyveri
1.3.1.109 36000
-
 x * 41000 + x * 36000 + x * 28000, during purification the butanoyl-CoA dehydrogenase/Etf complex partially dissociated into its components, SDS-PAGE Clostridium kluyveri
1.3.1.109 41000
-
 x * 41000 + x * 36000 + x * 28000, during purification the butanoyl-CoA dehydrogenase/Etf complex partially dissociated into its components, SDS-PAGE Clostridium kluyveri
1.3.1.109 320000
-
 gel filtration Clostridium kluyveri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.109 (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster Clostridium kluyveri
-
 butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.109 Clostridium kluyveri
-
-
-
1.3.8.1 Clostridium kluyveri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.109
-
 Clostridium kluyveri
1.3.8.1 Q-Sepharose column chromatography, hydroxyapatite column chromatography, Phenyl-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography Clostridium kluyveri

Storage Stability

EC Number Storage Stability Organism
1.3.1.109 4°C, 2-3 days, in the presence of FAD and under anoxic conditions the butyryl-CoA dehydrogenase/Etf complex loses 50% of its activity Clostridium kluyveri
1.3.8.1 4°C, in the presence of FAD and under anoxic conditions, 2-3 days, 50% loss of activity Clostridium kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.109 (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
 Clostridium kluyveri butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 ?
1.3.1.109 (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster the enzyme couples the endergonic reduction of ferredoxin with NADH to the exergonic reduction of crotonyl-CoA to butanoyl-CoA Clostridium kluyveri butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 ?
1.3.8.1 butyryl-CoA + ferrocenium
-
 Clostridium kluyveri 2-butenoyl-CoA + ferrocene
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.109 ? x * 41000 + x * 36000 + x * 28000, during purification the butanoyl-CoA dehydrogenase/Etf complex partially dissociated into its components, SDS-PAGE Clostridium kluyveri

Synonyms

EC Number Synonyms Comment Organism
1.3.1.109 butyryl-CoA dehydrogenase/Etf complex
-
 Clostridium kluyveri
1.3.8.1 butyryl-CoA dehydrogenase/Etf complex
-
 Clostridium kluyveri

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.1 FAD
-
 Clostridium kluyveri