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Literature summary extracted from

  • Moonen, M.J.; Kamerbeek, N.M.; Westphal, A.H.; Boeren, S.A.; Janssen, D.B.; Fraaije, M.W.; van Berkel, W.J.
    Elucidation of the 4-hydroxyacetophenone catabolic pathway in Pseudomonas fluorescens ACB (2008), J. Bacteriol., 190, 5190-5198.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.2.1.61 additional information HapE shows 45% sequence identity with CymC, a p-cumic aldehyde dehydrogenase, from Pseudomonas putida Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.84 gne hapA, encoded in the hapCDEFGHIBA gene cluster, DNA and amino acid sequence determination and analysis Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.61 52400
-
-
 Pseudomonas fluorescens
1.14.13.84 71957
-
 x * 71957, sequence calculation Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.84 4-hydroxyacetophenone + NADPH + H+ + O2 Pseudomonas fluorescens the enzyme catalyzes the first committed step in th 4-hydroxyacetophenone catabolism, pathway overview 4-hydroxyphenyl acetate + NADP+ + H2O
-
 ?
1.14.13.84 4-hydroxyacetophenone + NADPH + H+ + O2 Pseudomonas fluorescens ACB the enzyme catalyzes the first committed step in th 4-hydroxyacetophenone catabolism, pathway overview 4-hydroxyphenyl acetate + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.61 Pseudomonas fluorescens
-
-
-
1.2.1.61 Pseudomonas fluorescens ACB
-
-
-
1.14.13.84 Pseudomonas fluorescens Q93TJ5 gene hapA, formerly hapE, encoded in the gene cluster hapCDEFGHIBA
-
1.14.13.84 Pseudomonas fluorescens ACB Q93TJ5 gene hapA, formerly hapE, encoded in the gene cluster hapCDEFGHIBA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.61 4-hydroxymuconic semialdehyde + NAD+ + H2O
-
 Pseudomonas fluorescens maleylacetate + NADH + H+
-
 ?
1.2.1.61 4-hydroxymuconic semialdehyde + NAD+ + H2O
-
 Pseudomonas fluorescens ACB maleylacetate + NADH + H+
-
 ?
1.14.13.84 4-hydroxyacetophenone + NADPH + H+ + O2
-
 Pseudomonas fluorescens 4-hydroxyphenyl acetate + NADP+ + H2O
-
 ?
1.14.13.84 4-hydroxyacetophenone + NADPH + H+ + O2 the enzyme catalyzes the first committed step in th 4-hydroxyacetophenone catabolism, pathway overview Pseudomonas fluorescens 4-hydroxyphenyl acetate + NADP+ + H2O
-
 ?
1.14.13.84 4-hydroxyacetophenone + NADPH + H+ + O2
-
 Pseudomonas fluorescens ACB 4-hydroxyphenyl acetate + NADP+ + H2O
-
 ?
1.14.13.84 4-hydroxyacetophenone + NADPH + H+ + O2 the enzyme catalyzes the first committed step in th 4-hydroxyacetophenone catabolism, pathway overview Pseudomonas fluorescens ACB 4-hydroxyphenyl acetate + NADP+ + H2O
-
 ?
1.14.13.84 additional information the enzyme is active with a wide range of aromatic and aliphatic ketones Pseudomonas fluorescens ?
-
 ?
1.14.13.84 additional information the enzyme is active with a wide range of aromatic and aliphatic ketones Pseudomonas fluorescens ACB ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.84 ? x * 71957, sequence calculation Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
1.2.1.61 4-hydroxymuconic semialdehyde dehydrogenase
-
 Pseudomonas fluorescens
1.2.1.61 hAPE
-
 Pseudomonas fluorescens
1.14.13.84 hAPA
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.84 25
-
 assay at Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.84 7
-
 assay at Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.61 NAD+
-
 Pseudomonas fluorescens
1.14.13.84 NADPH
-
 Pseudomonas fluorescens