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Literature summary extracted from

  • Wakamatsu, T.; Nakagawa, N.; Kuramitsu, S.; Masui, R.
    Structural basis for different substrate specificities of two ADP-ribose pyrophosphatases from Thermus thermophilus HB8 (2008), J. Bacteriol., 190, 1108-1117.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.13 expression of Ndx2 in Escherichia coli strain BL21(DE3) Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.13 Ndx2 alone and in complex with Mg2+, with Mg2+ and AMP, and with Mg2+ and a nonhydrolyzable ADPR analogue, hanging-drop vapor diffusion method, 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 100 mM KCl, 0.001 ml of protein solution is mixed with the equal volume of reservoir solution and equilibrated against the reservoir, containing 0.1 M MES, pH 6.5, 0.16 M sodium acetate or magnesium acetate for the complexed enzyme, 14% PEG 8000, and 20% glycerol, at 20°C, soaking of crystals in 50 mM KAu(CN)2, X-ray diffraction structure determination and anaylsis at 2.0 A resolution, MAD phasing, model building, and refinement Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.13 0.25
-
 ADP-ribose pH 9.0, 25°C Thermus thermophilus
3.6.1.13 0.3
-
 FAD pH 7.5, 25°C Thermus thermophilus
3.6.1.13 0.33
-
 FAD pH 9.0, 25°C Thermus thermophilus
3.6.1.13 0.52
-
 ADP-ribose pH 7.5, 25°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.13 Mg2+ binding structure, overview Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.13 ADP-ribose + H2O Thermus thermophilus
-
 AMP + D-ribose 5-phosphate
-
 ?
3.6.1.13 ADP-ribose + H2O Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 AMP + D-ribose 5-phosphate
-
 ?
3.6.1.13 FAD + H2O Thermus thermophilus preferred substrate ?
-
 ?
3.6.1.13 FAD + H2O Thermus thermophilus HB8 / ATCC 27634 / DSM 579 preferred substrate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.13 Thermus thermophilus Q84CU3
-
-
3.6.1.13 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q84CU3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.13 recombinant Ndx2 from Escherichia coli strain BL21(DE3) by heat treatment at 70°C for 15 min and ion exchange chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.13 ADP-glucose + H2O low activity Thermus thermophilus ?
-
 ?
3.6.1.13 ADP-glucose + H2O low activity Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
3.6.1.13 ADP-ribose + H2O
-
 Thermus thermophilus AMP + D-ribose 5-phosphate
-
 ?
3.6.1.13 ADP-ribose + H2O preferred substrate, substrate recognition and binding structure, overview Thermus thermophilus AMP + D-ribose 5-phosphate
-
 ?
3.6.1.13 ADP-ribose + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + D-ribose 5-phosphate
-
 ?
3.6.1.13 ADP-ribose + H2O preferred substrate, substrate recognition and binding structure, overview Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + D-ribose 5-phosphate
-
 ?
3.6.1.13 FAD + H2O preferred substrate Thermus thermophilus ?
-
 ?
3.6.1.13 FAD + H2O preferred substrate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.13 ADPR pyrophosphatase
-
 Thermus thermophilus
3.6.1.13 ADPRase
-
 Thermus thermophilus
3.6.1.13 Ndx2
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.13 25
-
 assay at Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.13 1.4
-
 ADP-ribose pH 7.5, 25°C Thermus thermophilus
3.6.1.13 2.5
-
 FAD pH 7.5, 25°C Thermus thermophilus
3.6.1.13 4.9
-
 ADP-ribose pH 9.0, 25°C Thermus thermophilus
3.6.1.13 5
-
 FAD pH 9.0, 25°C Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.13 9
-
-
 Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.13 7.5 9
-
 Thermus thermophilus