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Literature summary extracted from

  • Soemphol, W.; Toyama, H.; Moonmangmee, D.; Adachi, O.; Matsushita, K.
    L-sorbose reductase and its transcriptional regulator involved in L-sorbose utilization of Gluconobacter frateurii (2007), J. Bacteriol., 189, 4800-4808.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.289 genes sldSLC and sboA, DNA and amino acid sequence determination and analysis, genetic organization, a putational transcription regulator encoded by gene sboR is located upstream, sboRA comprises an operon, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Gluconobacter frateurii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.289 additional information construction of disruption mutants, a mutant defective in sboA shows significantly reduced growth on L-sorbose, while a sboR mutant grows on L-sorbose even better than the wild-type strain with higher NADPH-SR activity in cytoplasm fractions Gluconobacter frateurii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.289 cytoplasm
-
 Gluconobacter frateurii 5737
-
1.1.1.289 membrane
-
 Gluconobacter frateurii 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.289 28320
-
 3 * 29000, recombinant SboA, SDS-PAGE, 3 * 28320, SboA, sequence calculation Gluconobacter frateurii
1.1.1.289 29000
-
 3 * 29000, recombinant SboA, SDS-PAGE, 3 * 28320, SboA, sequence calculation Gluconobacter frateurii
1.1.1.289 87000
-
 recombinant SboA, gel filtration Gluconobacter frateurii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.289 D-glucitol + NADP+ Gluconobacter frateurii
-
 D-sorbose + NADPH + H+
-
 r
1.1.1.289 D-glucitol + NADP+ Gluconobacter frateurii
-
 L-sorbose + NADPH + H+
-
 r
1.1.1.289 D-glucitol + NADP+ Gluconobacter frateurii THD32
-
 D-sorbose + NADPH + H+
-
 r
1.1.1.289 D-glucitol + NADP+ Gluconobacter frateurii THD32
-
 L-sorbose + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.289 Gluconobacter frateurii
-
 genes sldSLC and sboA
-
1.1.1.289 Gluconobacter frateurii THD32
-
 genes sldSLC and sboA
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.289 additional information SboA is required for effective growth on D- or L-sorbose, the organism also grows on glycerol Gluconobacter frateurii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.289 D-glucitol + NADP+
-
 Gluconobacter frateurii D-sorbose + NADPH + H+
-
 r
1.1.1.289 D-glucitol + NADP+
-
 Gluconobacter frateurii THD32 D-sorbose + NADPH + H+
-
 r
1.1.1.289 D-glucitol + NADP+
-
 Gluconobacter frateurii L-sorbose + NADPH + H+
-
 r
1.1.1.289 D-glucitol + NADP+
-
 Gluconobacter frateurii THD32 L-sorbose + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.289 trimer 3 * 29000, recombinant SboA, SDS-PAGE, 3 * 28320, SboA, sequence calculation Gluconobacter frateurii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.289 FAD-dependent D-sorbitol dehydrogenase
-
 Gluconobacter frateurii
1.1.1.289 flavin adenine dinucleotide-dependent D-sorbitol dehydrogenase
-
 Gluconobacter frateurii
1.1.1.289 NADPH-dependent L-sorbose reductase
-
 Gluconobacter frateurii
1.1.1.289 NADPH-SR
-
 Gluconobacter frateurii
1.1.1.289 SboA
-
 Gluconobacter frateurii
1.1.1.289 SLDH
-
 Gluconobacter frateurii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.289 6 7 oxidation of L-sorbitol Gluconobacter frateurii
1.1.1.289 9
-
 reduction reaction, assay at Gluconobacter frateurii
1.1.1.289 10
-
 oxidation of D-sorbitol Gluconobacter frateurii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.289 FAD dependent on Gluconobacter frateurii
1.1.1.289 NADP+ dependent on Gluconobacter frateurii
1.1.1.289 NADPH dependent on Gluconobacter frateurii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.289 Gluconobacter frateurii SboA, sequence calculation
-
 5.72