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Literature summary extracted from

  • Vangnai, A.S.; Arp, D.J.; Sayavedra-Soto, L.A.
    Two distinct alcohol dehydrogenases participate in butane metabolism by Pseudomonas butanovora (2002), J. Bacteriol., 184, 1916-1924.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.5.2 additional information induction by alcohols, overview, BOH is induced by butanol and butane. When induced with butane, the gene for BOH is expressed early Thauera butanivorans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.5.2 gene boh, DNA and amino acid sequence determination and analysis Thauera butanivorans

Protein Variants

EC Number Protein Variants Comment Organism
1.2.5.2 additional information insertional disruption of boh affects, but not fully eliminates butane utilization in the mutant organism, the double boh/bdh mutant with both genes boh and bdh inactivated is unable to grow on butane or 1-butanol, but does, when grown in citrate and incubated in butane, develop butane oxidation capability and accumulates 1-butanol, overview Thauera butanivorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.9 periplasm
-
Thauera butanivorans
-
-
1.1.5.11 periplasm
-
Thauera butanivorans
-
-
1.2.5.2 periplasm BOH contains the periplasm 29-residue leader sequence Thauera butanivorans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.9 66000
-
x * 66000, SDS-PAGE Thauera butanivorans
1.1.2.9 70900
-
-
Thauera butanivorans
1.1.5.11 67553
-
-
Thauera butanivorans
1.2.5.2 67553
-
x * 67553, BOH, calculated from sequence Thauera butanivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.9 butan-1-ol + ferricytochrome c Thauera butanivorans
-
butanal + ferrocytochrome c + H+
-
?
1.2.5.2 additional information Thauera butanivorans the NAD+-independent PQQ alcohol dehydrogenase BOH (a quinoprotein) is linked to butane metabolism in conjunction with BDH (a quinohemoprotein) ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.9 Thauera butanivorans Q9AF95 ATCC 43655
-
1.1.5.11 Thauera butanivorans
-
-
-
1.1.5.11 Thauera butanivorans ATCC 43655
-
-
-
1.2.5.2 Thauera butanivorans Q9AGW3 1-butanol dehydrogenase, BOH; strain ATCC 43655, gene boh
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.9 butan-1-ol + ferricytochrome c
-
Thauera butanivorans butanal + ferrocytochrome c + H+
-
?
1.1.2.9 additional information no activity with 2-butanol Thauera butanivorans ?
-
?
1.1.5.11 1-octanol + a quinone 22% activity compared to butan-2-ol Thauera butanivorans octanal + a quinol
-
?
1.1.5.11 1-octanol + a quinone 22% activity compared to butan-2-ol Thauera butanivorans ATCC 43655 octanal + a quinol
-
?
1.1.5.11 1-pentanol + a quinone 34% activity compared to butan-2-ol Thauera butanivorans pentanal + a quinol
-
?
1.1.5.11 1-pentanol + a quinone 34% activity compared to butan-2-ol Thauera butanivorans ATCC 43655 pentanal + a quinol
-
?
1.1.5.11 1-propanol + a quinone 22% activity compared to butan-2-ol Thauera butanivorans propanal + a quinol
-
?
1.1.5.11 1-propanol + a quinone 22% activity compared to butan-2-ol Thauera butanivorans ATCC 43655 propanal + a quinol
-
?
1.1.5.11 butan-1-ol + a quinone 87% activity compared to butan-2-ol Thauera butanivorans butanal + a quinol
-
?
1.1.5.11 butan-1-ol + a quinone 87% activity compared to butan-2-ol Thauera butanivorans ATCC 43655 butanal + a quinol
-
?
1.1.5.11 butan-2-ol + a quinone 100% activity Thauera butanivorans 2-butanone + a quinol
-
?
1.1.5.11 butan-2-ol + a quinone 100% activity Thauera butanivorans ATCC 43655 2-butanone + a quinol
-
?
1.1.5.11 butyraldehyde + a quinol 25% activity compared to butan-2-ol Thauera butanivorans ?
-
?
1.1.5.11 ethanol + a quinone 0.7% activity compared to butan-2-ol Thauera butanivorans acetaldehyde + a quinol
-
?
1.1.5.11 methanol + a quinone 0.7% activity compared to butan-2-ol Thauera butanivorans formaldehyde + a quinol
-
?
1.1.5.11 octan-2-ol + a quinone 15% activity compared to butan-2-ol Thauera butanivorans 2-octanone + a quinol
-
?
1.1.5.11 pentan-2-ol + a quinone 61% activity compared to butan-2-ol Thauera butanivorans 2-pentanone + a quinol
-
?
1.1.5.11 propan-2-ol + a quinone 0.7% activity compared to butan-2-ol Thauera butanivorans acetone + a quinol
-
?
1.1.5.11 propionaldehyde + a quinol 33% activity compared to butan-2-ol Thauera butanivorans ?
-
?
1.2.5.2 butyraldehyde + 2,6-dichlorophenolindophenol
-
Thauera butanivorans butanoate + reduced 2,6-dichlorophenolindophenol
-
?
1.2.5.2 additional information the NAD+-independent PQQ alcohol dehydrogenase BOH (a quinoprotein) is linked to butane metabolism in conjunction with BDH (a quinohemoprotein) Thauera butanivorans ?
-
?
1.2.5.2 propionaldehyde + 2,6-dichlorophenolindophenol 30% of the activity with 2-butanol Thauera butanivorans propanoate + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

EC Number Subunits Comment Organism
1.1.2.9 ? x * 66000, SDS-PAGE Thauera butanivorans
1.1.2.9 ? x * 70900, calculated from amino acid sequence Thauera butanivorans
1.1.5.11 ? x * 67553, calculated from amino acid sequence Thauera butanivorans
1.2.5.2 ? x * 67553, BOH, calculated from sequence Thauera butanivorans

Synonyms

EC Number Synonyms Comment Organism
1.1.2.9 1-butanol dehydrogenase
-
Thauera butanivorans
1.1.2.9 BDH
-
Thauera butanivorans
1.1.5.5 BOH
-
-
1.1.5.5 NAD+-independent, PQQ-containing alcohol dehydrogenase
-
-
1.1.5.11 1-butanol dehydrogenase
-
Thauera butanivorans
1.1.5.11 BDH
-
-
1.1.5.11 BOH
-
Thauera butanivorans
1.2.5.2 BOH
-
Thauera butanivorans
1.2.5.2 NAD+-independent, PQQ-containing alcohol dehydrogenase
-
Thauera butanivorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.9 heme c
-
Thauera butanivorans
1.1.2.9 pyrroloquinoline quinone
-
Thauera butanivorans
1.1.5.11 additional information NAD+-independent Thauera butanivorans
1.1.5.11 pyrroloquinoline quinone
-
Thauera butanivorans
1.2.5.2 additional information BOH contains no heme c Thauera butanivorans
1.2.5.2 pyrroloquinoline quinone BOH is a quinoprotein Thauera butanivorans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.2.9 Thauera butanivorans calculated from amino acid sequence
-
6.7
1.1.5.11 Thauera butanivorans calculated from amino acid sequence
-
6.2

Expression

EC Number Organism Comment Expression
1.1.2.9 Thauera butanivorans enzyme mRNA is induced when the cell's 1-butanol oxidation activity is induced up
1.1.5.11 Thauera butanivorans the enzyme's mRNAs is induced when the cell's 1-butanol oxidation activity is induced up

General Information

EC Number General Information Comment Organism
1.1.2.9 malfunction insertional disruption of the enzyme gene affects adversely, but does not eliminate, butane utilization Thauera butanivorans
1.1.5.11 malfunction insertional disruption of the enzyme gene adversely, but does not eliminate, butane utilization Thauera butanivorans