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Literature summary extracted from

  • Watabe, K.; Ishikawa, T.; Mukohara, Y.; Nakamura, H.
    Purification and characterization of the hydantoin racemase of Pseudomonas sp. strain NS671 expressed in Escherichia coli (1992), J. Bacteriol., 174, 7989-7995.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.99.5 expression in Escherichia coli Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.99.5 Cu2+ strong inhibition Pseudomonas sp.
5.1.99.5 DL-5-isopropylhydantoin complete inhibition, pre-incubation with DL-5-(2-methylthioethyl)hydantoin protects Pseudomonas sp.
5.1.99.5 Zn2+ strong inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.99.5 29.8
-
 D-5-(2-methylthioethyl)hydantoin pH 7.5, 30°C Pseudomonas sp.
5.1.99.5 79.7
-
 L-5-(2-methylthioethyl)hydantoin pH 7.5, 30°C Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.99.5 Co2+ slight stimulation Pseudomonas sp.
5.1.99.5 Mn2+ slight stimulation Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.99.5 32000
-
 x * 32000, SDS-PAGE Pseudomonas sp.
5.1.99.5 190000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
5.1.99.5 Pseudomonas sp. Q00924
-
-
5.1.99.5 Pseudomonas sp. NS671 Q00924
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.99.5 20.8
-
 pH 7.5, 30°C Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
5.1.99.5 4°C, ammonium sulfate at 90% saturation, stable for 6 months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.99.5 D-5-isobutylhydantoin complete racemization Pseudomonas sp. L-5-isobutylhydantoin
-
 r
5.1.99.5 D-5-isobutylhydantoin complete racemization Pseudomonas sp. NS671 L-5-isobutylhydantoin
-
 r
5.1.99.5 D-5-methylhydantoin complete racemization Pseudomonas sp. L-5-methylhydantoin
-
 r
5.1.99.5 D-5-methylhydantoin complete racemization Pseudomonas sp. NS671 L-5-methylhydantoin
-
 r
5.1.99.5 L-5-(2-methylthioethyl)hydantoin complete racemization Pseudomonas sp. D-5-(2-methylthioethyl)hydantoin
-
 r
5.1.99.5 L-5-(2-methylthioethyl)hydantoin complete racemization Pseudomonas sp. NS671 D-5-(2-methylthioethyl)hydantoin
-
 r
5.1.99.5 L-5-methylhydantoin about 50% racemization Pseudomonas sp. D-5-methylhydantoin
-
 r
5.1.99.5 L-5-methylhydantoin about 50% racemization Pseudomonas sp. NS671 D-5-methylhydantoin
-
 r
5.1.99.5 additional information no substrate: DL-5-isopropylhydantoin Pseudomonas sp. ?
-
 ?
5.1.99.5 additional information no substrate: DL-5-isopropylhydantoin Pseudomonas sp. NS671 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.99.5 hexamer x * 32000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.99.5 45
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.99.5 45
-
 in presence of substrate, stable Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.99.5 9.5
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.99.5 11
-
 95% of maximum activity Pseudomonas sp.