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Literature summary extracted from

  • Reece, S.Y.; Seyedsayamdost, M.R.; Stubbe, J.; Nocera, D.G.
    Photoactive peptides for light-initiated tyrosyl radical generation and transport into ribonucleotide reductase (2007), J. Am. Chem. Soc., 129, 8500-8509.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 ATP ATP is an allosteric effector Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.17.4.1 Y730F site-directed mutagenesis, the mutant excludes a direct superexchange mechanism between C439 and Y731 in radical transport, overview Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 peptide Y-R2C19 a 20-mer peptide, which is identical to the C-terminal peptide tail of the R2 subunit and is a known competitive inhibitor of binding of the native R2 protein to R1 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.1 CDP + thioredoxin Escherichia coli
-
 2'-deoxyCDP + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.17.4.1 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin reaction mechanism via formation of a tyrosyl radical at Tyr356 od subunit R1, model for radical transport in which there is a unidirectional transport of the electron and proton transport among residues of R1 Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 CDP + thioredoxin
-
 Escherichia coli 2'-deoxyCDP + thioredoxin disulfide + H2O
-
 ?
1.17.4.1 CDP + thioredoxin coupled assay method with NADPH, transient spectrometric measurement of Y356 radical intermediate, Y356 radical initiation is prompted by excitation of a proximal anthraquinone or benzophenone chromophore on a 20-mer peptide Y-R2C19, bound to subunit alpha2, both the Anq and BPA-containing peptides are competent in deoxycytidine diphosphate formation and turnover occurs via Y731 to Y730 to C439 pathway-dependent radical transport in R1, overview. Peptide Y-R2C19 is identical to the C-terminal peptide tail of the R2 subunit and is a known competitive inhibitor of binding of the native R2 protein to R1 Escherichia coli 2'-deoxyCDP + thioredoxin disulfide + H2O
-
 ?
1.17.4.1 additional information mechanism of radical transport in the R1 subunit of the class I enzyme, overview Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.4.1 tetramer 2 * subunit R1 + 2 * subunit R2 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.17.4.1 More class I ribonucleotide reductase Escherichia coli
1.17.4.1 RNR
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.1 7.6
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.1 thioredoxin
-
 Escherichia coli