EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.99.38 | expression in Escherichia coli | Niallia circulans |
1.3.98.3 | expressed in Escherichia coli strain BL21(DE3) | Niallia circulans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.38 | 2-deoxy-scyllo-inosamine | substrate inhibition | Niallia circulans | |
1.3.98.3 | 2-deoxy-scyllo-inosamine | uncompetitive substrate inhibition | Niallia circulans | |
1.3.98.3 | additional information | not inhibited by S-adenosyl-L-methionine | Niallia circulans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.38 | 0.022 | - |
2-deoxy-scyllo-inosamine | pH 8.0, 28°C | Niallia circulans | |
1.1.99.38 | 0.46 | - |
S-adenosyl-L-methionine | pH 8.0, 28°C | Niallia circulans | |
1.3.98.3 | 0.022 | - |
2-deoxy-scyllo-inosamine | in 50 mM HEPES-NaOH (pH 8.0) in the presence of 10 mM sodium dithionite at 28°C | Niallia circulans | |
1.3.98.3 | 0.46 | - |
S-adenosyl-L-methionine | apparent value, in 50 mM HEPES-NaOH (pH 8.0) in the presence of 10 mM sodium dithionite at 28°C | Niallia circulans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.38 | Niallia circulans | Q8G907 | - |
- |
1.3.98.3 | Niallia circulans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.98.3 | His-bind resin column chromatography | Niallia circulans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.38 | 2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine | enzyme catalyzes the oxidation of 2-deoxy-scyllo-inosamine under strictly anaerobic conditions along with consumption of an equimolar amount of S-adenosyl-L-methionine to produce 5-deoxyadenosine, methionine, and 3-amino-2,3-dideoxy-scylloinososeReaction follows the ordered Bi Ter mechanism and S-adenosyl-L-methionine is the first substrate and 2-deoxy-scyllo-inosamine is the second. The hydrogen atom at C-3 of 2-deoxy-scyllo-inosamine is directly transferred to 5-deoxyadenosine to give the radical intermediate of 2-deoxy-scyllo-inosamine in a reversible hydrogen abstraction step | Niallia circulans | 3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine | - |
? | |
1.1.99.38 | 2-deoxystreptamine + S-adenosyl-L-methionine | 14.4% of the rate with 2-deoxy-scyllo-inosamine | Niallia circulans | ? + 5'-deoxyadenosine + L-methionine | - |
? | |
1.1.99.38 | additional information | no substrates: 1D-chiro-inositol, 1L-chiro-inositol, muco-inositol, allo-inositol, D-glucose, D-glucosamine, D-xylose | Niallia circulans | ? | - |
? | |
1.1.99.38 | myo-inositol + S-adenosyl-L-methionine | 0.9% of the rate with 2-deoxy-scyllo-inosamine | Niallia circulans | ? + 5'-deoxyadenosine + L-methionine | - |
? | |
1.1.99.38 | scyllo-inositol + S-adenosyl-L-methionine | 3.3% of the rate with 2-deoxy-scyllo-inosamine | Niallia circulans | ? + 5'-deoxyadenosine + L-methionine | - |
? | |
1.3.98.3 | 2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine | 100% activity | Niallia circulans | 3-amino-2,3-dideoxy-scyllo-inosose + CO2 + L-methionine + 5'-deoxyadenosine | - |
? | |
1.3.98.3 | 2-deoxystreptamine + S-adenosyl-L-methionine | 14.4% activity compared to 2-deoxy-scyllo-inosamine | Niallia circulans | ? + CO2 + L-methionine + 5'-deoxyadenosine | - |
? | |
1.3.98.3 | additional information | no activity with 1L-chiro-inositol, muco-inositol, allo-inositol, D-glucose, D-glucosamine, D-xylose, 1D-chiro-inositol, and 2,3-dideoxy-scyllo-inosose | Niallia circulans | ? | - |
? | |
1.3.98.3 | myo-inositol + S-adenosyl-L-methionine | 0.9% activity compared to 2-deoxy-scyllo-inosamine | Niallia circulans | ? + CO2 + L-methionine + 5'-deoxyadenosine | - |
? | |
1.3.98.3 | scyllo-inositol + S-adenosyl-L-methionine | 3.3% activity compared to 2-deoxy-scyllo-inosamine | Niallia circulans | ? + CO2 + L-methionine + 5'-deoxyadenosine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.38 | BtrN | - |
Niallia circulans |
1.3.98.3 | BtrN | BtrN is an unusual radical SAM dehydrogenase catalyzing the oxidation of the hydroxyl group by a radical mechanism | Niallia circulans |
1.3.98.3 | oxygen-independent coproporphyrinogen-III oxidase | - |
Niallia circulans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.38 | 0.02 | - |
S-adenosyl-L-methionine | pH 8.0, 28°C | Niallia circulans | |
1.1.99.38 | 0.033 | - |
2-deoxy-scyllo-inosamine | pH 8.0, 28°C | Niallia circulans | |
1.3.98.3 | 0.02 | - |
S-adenosyl-L-methionine | apparent value, in 50 mM HEPES-NaOH (pH 8.0) in the presence of 10 mM sodium dithionite at 28°C | Niallia circulans | |
1.3.98.3 | 0.038 | - |
2-deoxy-scyllo-inosamine | in 50 mM HEPES-NaOH (pH 8.0) in the presence of 10 mM sodium dithionite at 28°C | Niallia circulans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.98.3 | 4Fe-4S-center | - |
Niallia circulans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.99.38 | physiological function | gene disruption causes the interruption of the biosynthetic pathway between 2-deoxy-scyllo-inosamine and 2-deoxystreptamine. In vitro assay of the overexpressed enzyme reveals that the enzyme catalyzes the oxidation of 2-deoxy-scyllo-inosamine under strictly anaerobic conditions along with consumption of an equimolar amount of S-adenosyl-L-methionine to produce 5-deoxyadenosine, methionine, and 3-amino-2,3-dideoxy-scyllo-inosose | Niallia circulans |