EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.5.1 | expression in Escherichia coli | Ipomoea batatas |
EC Number | General Stability | Organism |
---|---|---|
1.8.5.1 | resistant to digestion by trypsin and chymotrypsin even at a high enzyme/substrate (w/w) ratio of 1:10 | Ipomoea batatas |
1.8.5.1 | slight decrease in activity with increasing concentration of imidazole to 0.8 M | Ipomoea batatas |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.5.1 | 0.19 | - |
dehydroascorbate | 25°C | Ipomoea batatas | |
1.8.5.1 | 2.38 | - |
glutathione | 25°C | Ipomoea batatas |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.5.1 | 25000 | - |
1 * 25000, SDS-PAGE | Ipomoea batatas |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.5.1 | Ipomoea batatas | D2CGD4 | (L.) Lam | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.5.1 | recombinant enzyme | Ipomoea batatas |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.5.1 | 52 | - |
- |
Ipomoea batatas |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.5.1 | glutathione + dehydroascorbate | - |
Ipomoea batatas | glutathione disulfide + ascorbate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.5.1 | monomer | 1 * 25000, SDS-PAGE | Ipomoea batatas |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.5.1 | dehydroascorbate reductase | - |
Ipomoea batatas |
1.8.5.1 | DHAR | - |
Ipomoea batatas |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.5.1 | 50 | - |
half-life: 10.1 min | Ipomoea batatas |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.5.1 | 100.6 | - |
glutathione | 25°C | Ipomoea batatas | |
1.8.5.1 | 368.2 | - |
dehydroascorbate | 25°C | Ipomoea batatas |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.8.5.1 | 6 | 11 | stable | Ipomoea batatas |