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Literature summary extracted from

  • Jiang, Y.C.; Huang, C.Y.; Wen, L.; Lin, C.T.
    Dehydroascorbate reductase cDNA from sweet potato (Ipomoea batatas [L.] Lam): expression, enzyme properties, and kinetic studies (2008), J. Agric. Food Chem., 56, 3623-3627.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.1 expression in Escherichia coli Ipomoea batatas

General Stability

EC Number General Stability Organism
1.8.5.1 resistant to digestion by trypsin and chymotrypsin even at a high enzyme/substrate (w/w) ratio of 1:10 Ipomoea batatas
1.8.5.1 slight decrease in activity with increasing concentration of imidazole to 0.8 M Ipomoea batatas

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.1 0.19
-
dehydroascorbate 25°C Ipomoea batatas
1.8.5.1 2.38
-
glutathione 25°C Ipomoea batatas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.5.1 25000
-
1 * 25000, SDS-PAGE Ipomoea batatas

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.1 Ipomoea batatas D2CGD4 (L.) Lam
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.1 recombinant enzyme Ipomoea batatas

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.5.1 52
-
-
Ipomoea batatas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.1 glutathione + dehydroascorbate
-
Ipomoea batatas glutathione disulfide + ascorbate
-
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Subunits

EC Number Subunits Comment Organism
1.8.5.1 monomer 1 * 25000, SDS-PAGE Ipomoea batatas

Synonyms

EC Number Synonyms Comment Organism
1.8.5.1 dehydroascorbate reductase
-
Ipomoea batatas
1.8.5.1 DHAR
-
Ipomoea batatas

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.5.1 50
-
half-life: 10.1 min Ipomoea batatas

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.5.1 100.6
-
glutathione 25°C Ipomoea batatas
1.8.5.1 368.2
-
dehydroascorbate 25°C Ipomoea batatas

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.8.5.1 6 11 stable Ipomoea batatas