BRENDA - Enzyme Database

The PQQ-alcohol dehydrogenase of Gluconacetobacter diazotrophicus

Gomez-Manzo, S.; Contreras-Zentella, M.; Gonzalez-Valdez, A.; Sosa-Torres, M.; Arreguin-Espinoza, R.; Escamilla-Marvan, E.; Int. J. Food Microbiol. 125, 71-78 (2008)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.5
membrane
bound
Gluconacetobacter diazotrophicus
16020
-
1.2.5.2
membrane
bound
Gluconacetobacter diazotrophicus
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.5
43500
-
1 * 71400 + 1 * 43500, SDS-PAGE
Gluconacetobacter diazotrophicus
1.1.5.5
71400
-
1 * 71400 + 1 * 43500, SDS-PAGE
Gluconacetobacter diazotrophicus
1.1.5.5
115000
-
non-denaturing PAGE
Gluconacetobacter diazotrophicus
1.2.5.2
43500
-
1 * 71400 + 1 * 43500, SDS-PAGE
Gluconacetobacter diazotrophicus
1.2.5.2
71400
-
1 * 71400 + 1 * 43500, SDS-PAGE
Gluconacetobacter diazotrophicus
1.2.5.2
115000
-
non-denaturing PAGE
Gluconacetobacter diazotrophicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.5.2
acetaldehyde + ubiquinone
Gluconacetobacter diazotrophicus
electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c to the membrane ubiquinone
acetate + ubiquinol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.5.5
Gluconacetobacter diazotrophicus
-
-
-
1.2.5.2
Gluconacetobacter diazotrophicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.5.5
-
Gluconacetobacter diazotrophicus
1.2.5.2
-
Gluconacetobacter diazotrophicus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.5.5
258
-
-
Gluconacetobacter diazotrophicus
1.2.5.2
258
-
-
Gluconacetobacter diazotrophicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.5
allyl alcohol + ferricyanide
the best substrate
687166
Gluconacetobacter diazotrophicus
acrolein + ferricyanide
-
-
-
?
1.1.5.5
ethanol + ferricyanide
95% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
acetaldehyde + ferrocyanide
-
-
-
?
1.1.5.5
isopropanol + ferricyanide
18% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
propan-2-one + ferrocyanide
-
-
-
?
1.1.5.5
methanol + ferricyanide
9% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
formaldehyde + ferrocyanide
-
-
-
?
1.1.5.5
additional information
no activity with glucose, benzaldehyde, formaldehyde, acetone, sorbitol or glycerol
687166
Gluconacetobacter diazotrophicus
?
-
-
-
-
1.1.5.5
n-butanol + ferricyanide
98% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
butyraldehyde + ferrocyanide
-
-
-
?
1.1.5.5
n-propanol + ferricyanide
90% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
propionaldehyde + ferrocyanide
-
-
-
?
1.2.5.2
acetaldehyde + 2 ferricyanide + H2O
87% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
acetate + 2 ferrocyanide + 2 H+
-
-
-
?
1.2.5.2
acetaldehyde + ubiquinone
electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c to the membrane ubiquinone
687166
Gluconacetobacter diazotrophicus
acetate + ubiquinol
-
-
-
?
1.2.5.2
glutaraldehyde + 4 ferricyanide + 2 H2O
25% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
glutarate + 4 ferrocyanide + 4 H+
-
-
-
?
1.2.5.2
additional information
no activity with glucose, benzaldehyde, formaldehyde, acetone, sorbitol or glycerol
687166
Gluconacetobacter diazotrophicus
?
-
-
-
-
1.2.5.2
propanal + 2 ferricyanide + H2O
42% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
propanoate + 2 ferrocyanide + 2 H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.5.5
dimer
1 * 71400 + 1 * 43500, SDS-PAGE
Gluconacetobacter diazotrophicus
1.2.5.2
dimer
1 * 71400 + 1 * 43500, SDS-PAGE
Gluconacetobacter diazotrophicus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.5.5
40
-
15 min, 80% loss of activity
Gluconacetobacter diazotrophicus
1.2.5.2
40
-
15 min, 80% loss of activity
Gluconacetobacter diazotrophicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.5
6
-
substrate: ethanol
Gluconacetobacter diazotrophicus
1.2.5.2
6
-
substrate: acetaldehyde
Gluconacetobacter diazotrophicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.5.5
5
7.5
pH 5.0: about 50% of maximal activity, pH 7.5: about 55% of maximal activity, substrate: ethanol
Gluconacetobacter diazotrophicus
1.2.5.2
5
7.5
pH 5.0: about 50% of maximal activity, pH 7.5: about 55% of maximal activity, substrate: acetaldehyde
Gluconacetobacter diazotrophicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.5
cytochrome c
presence of cytochrome c in both subunits
Gluconacetobacter diazotrophicus
1.1.5.5
pyrroloquinoline quinone
electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c
Gluconacetobacter diazotrophicus
1.2.5.2
cytochrome c
presence of cytochrome c in both subunits
Gluconacetobacter diazotrophicus
1.2.5.2
pyrroloquinoline quinone
electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c to the membrane ubiquinone
Gluconacetobacter diazotrophicus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.5.5
Gluconacetobacter diazotrophicus
gradient electrophoresis, determined in pH range 3.4-9.0
-
6.1
1.2.5.2
Gluconacetobacter diazotrophicus
gradient electrophoresis, determined in pH range 3.4-9.0
-
6.1
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.5
cytochrome c
presence of cytochrome c in both subunits
Gluconacetobacter diazotrophicus
1.1.5.5
pyrroloquinoline quinone
electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c
Gluconacetobacter diazotrophicus
1.2.5.2
cytochrome c
presence of cytochrome c in both subunits
Gluconacetobacter diazotrophicus
1.2.5.2
pyrroloquinoline quinone
electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c to the membrane ubiquinone
Gluconacetobacter diazotrophicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.5
membrane
bound
Gluconacetobacter diazotrophicus
16020
-
1.2.5.2
membrane
bound
Gluconacetobacter diazotrophicus
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.5
43500
-
1 * 71400 + 1 * 43500, SDS-PAGE
Gluconacetobacter diazotrophicus
1.1.5.5
71400
-
1 * 71400 + 1 * 43500, SDS-PAGE
Gluconacetobacter diazotrophicus
1.1.5.5
115000
-
non-denaturing PAGE
Gluconacetobacter diazotrophicus
1.2.5.2
43500
-
1 * 71400 + 1 * 43500, SDS-PAGE
Gluconacetobacter diazotrophicus
1.2.5.2
71400
-
1 * 71400 + 1 * 43500, SDS-PAGE
Gluconacetobacter diazotrophicus
1.2.5.2
115000
-
non-denaturing PAGE
Gluconacetobacter diazotrophicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.5.2
acetaldehyde + ubiquinone
Gluconacetobacter diazotrophicus
electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c to the membrane ubiquinone
acetate + ubiquinol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.5
-
Gluconacetobacter diazotrophicus
1.2.5.2
-
Gluconacetobacter diazotrophicus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.5.5
258
-
-
Gluconacetobacter diazotrophicus
1.2.5.2
258
-
-
Gluconacetobacter diazotrophicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.5
allyl alcohol + ferricyanide
the best substrate
687166
Gluconacetobacter diazotrophicus
acrolein + ferricyanide
-
-
-
?
1.1.5.5
ethanol + ferricyanide
95% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
acetaldehyde + ferrocyanide
-
-
-
?
1.1.5.5
isopropanol + ferricyanide
18% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
propan-2-one + ferrocyanide
-
-
-
?
1.1.5.5
methanol + ferricyanide
9% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
formaldehyde + ferrocyanide
-
-
-
?
1.1.5.5
additional information
no activity with glucose, benzaldehyde, formaldehyde, acetone, sorbitol or glycerol
687166
Gluconacetobacter diazotrophicus
?
-
-
-
-
1.1.5.5
n-butanol + ferricyanide
98% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
butyraldehyde + ferrocyanide
-
-
-
?
1.1.5.5
n-propanol + ferricyanide
90% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
propionaldehyde + ferrocyanide
-
-
-
?
1.2.5.2
acetaldehyde + 2 ferricyanide + H2O
87% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
acetate + 2 ferrocyanide + 2 H+
-
-
-
?
1.2.5.2
acetaldehyde + ubiquinone
electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c to the membrane ubiquinone
687166
Gluconacetobacter diazotrophicus
acetate + ubiquinol
-
-
-
?
1.2.5.2
glutaraldehyde + 4 ferricyanide + 2 H2O
25% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
glutarate + 4 ferrocyanide + 4 H+
-
-
-
?
1.2.5.2
additional information
no activity with glucose, benzaldehyde, formaldehyde, acetone, sorbitol or glycerol
687166
Gluconacetobacter diazotrophicus
?
-
-
-
-
1.2.5.2
propanal + 2 ferricyanide + H2O
42% of the activity with allyl alcohol
687166
Gluconacetobacter diazotrophicus
propanoate + 2 ferrocyanide + 2 H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.5.5
dimer
1 * 71400 + 1 * 43500, SDS-PAGE
Gluconacetobacter diazotrophicus
1.2.5.2
dimer
1 * 71400 + 1 * 43500, SDS-PAGE
Gluconacetobacter diazotrophicus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.5.5
40
-
15 min, 80% loss of activity
Gluconacetobacter diazotrophicus
1.2.5.2
40
-
15 min, 80% loss of activity
Gluconacetobacter diazotrophicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.5
6
-
substrate: ethanol
Gluconacetobacter diazotrophicus
1.2.5.2
6
-
substrate: acetaldehyde
Gluconacetobacter diazotrophicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.5.5
5
7.5
pH 5.0: about 50% of maximal activity, pH 7.5: about 55% of maximal activity, substrate: ethanol
Gluconacetobacter diazotrophicus
1.2.5.2
5
7.5
pH 5.0: about 50% of maximal activity, pH 7.5: about 55% of maximal activity, substrate: acetaldehyde
Gluconacetobacter diazotrophicus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.5.5
Gluconacetobacter diazotrophicus
gradient electrophoresis, determined in pH range 3.4-9.0
-
6.1
1.2.5.2
Gluconacetobacter diazotrophicus
gradient electrophoresis, determined in pH range 3.4-9.0
-
6.1