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Literature summary extracted from

  • Brizio, C.; Brandsch, R.; Douka, M.; Wait, R.; Barile, M.
    The purified recombinant precursor of rat mitochondrial dimethylglycine dehydrogenase binds FAD via an autocatalytic reaction (2008), Int. J. Biol. Macromol., 42, 455-462.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.8.4 expressed in Escherichia coli BL21(DE3) cells as a C-terminally 6-His-tagged fusion protein which is predominantly in its apo form Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.8.4 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.8.4 93000
-
 His-tagged mMe2GlyDH, SDS-PAGE Rattus norvegicus
1.5.8.4 94000
-
 His-tagged pMe2GlyDH, SDS-PAGE Rattus norvegicus
1.5.8.4 97410
-
 pMe2GlyDH, calculated from amino acid sequence Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.4 Rattus norvegicus Q63342
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.4 Ni2+-Sepharose column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.4 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.4 N,N-dimethylglycine + acceptor + H2O
-
 Rattus norvegicus sarcosine + formaldehyde + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.8.4 mMe2GlyDH mature dimethylglycine dehydrogenase Rattus norvegicus
1.5.8.4 pMe2GlyDH precursor of dimethylglycine dehydrogenase Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.8.4 FAD FAD is covalently bound via an autocatalytic reaction Rattus norvegicus