EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.12.10 | when expressed in Escherichia coli, benzoate 1,2-dioxygenase transforms benzoate into cis-benzoate dihydrodiol | Chromohalobacter sp. |
1.14.13.2 | When expressed in Escherichia coli, p-hydroxybenzoate hydroxylase transforms p-hydroxybenzoate into protocatechuate | Chromohalobacter sp. |
1.14.14.92 | PCR amplification, expression in Escherichia coli BL21 (DE3) | Chromohalobacter sp. HS-2 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.92 | NaCl | 10% is optimal NaCl concentration for Chromohalobacter sp. strain HS-2 | Chromohalobacter sp. HS-2 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.3 | additional information | Chromohalobacter sp. HS-2 | benzoate induces expression of protocatechuate 3,4-dioxygenase | ? | - |
? | |
1.14.12.10 | additional information | Chromohalobacter sp. | benzoate induces the expression of benzoate 1,2-dioxygenase | ? | - |
? | |
1.14.13.2 | additional information | Chromohalobacter sp. | p-hydroxybenzoate induces the expression of p-hydroxybenzoate hydroxylase | ? | - |
? | |
1.14.14.92 | benzoate + [reduced NADPH-hemoprotein reductase] + O2 | Chromohalobacter sp. HS-2 | - |
2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further | ? | |
1.14.14.92 | benzoate + [reduced NADPH-hemoprotein reductase] + O2 | Chromohalobacter sp. HS2 | - |
2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.3 | Chromohalobacter sp. HS-2 | A8I4B3 | protocatechuate 3,4-dioxygenase beta subunit | - |
1.13.11.3 | Chromohalobacter sp. HS-2 | A8I4B7 | protocatechuate 3,4-dioxygenase alpha subunit | - |
1.14.12.10 | Chromohalobacter sp. | - |
- |
- |
1.14.13.2 | Chromohalobacter sp. | - |
- |
- |
1.14.14.92 | Chromohalobacter sp. HS-2 | A8I4E8 | ben A subunits; isolated from salted fermented clams | - |
1.14.14.92 | Chromohalobacter sp. HS-2 | A8I4F1 | ben B subunits; isolated from salted fermented clams | - |
1.14.14.92 | Chromohalobacter sp. HS-2 | A8I4F4 | ben C subunits; isolated from salted fermented clams | - |
1.14.14.92 | Chromohalobacter sp. HS2 | A8I4E8 | ben A subunits; isolated from salted fermented clams | - |
1.14.14.92 | Chromohalobacter sp. HS2 | A8I4F1 | ben B subunits; isolated from salted fermented clams | - |
1.14.14.92 | Chromohalobacter sp. HS2 | A8I4F4 | ben C subunits; isolated from salted fermented clams | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.3 | additional information | benzoate induces expression of protocatechuate 3,4-dioxygenase | Chromohalobacter sp. HS-2 | ? | - |
? | |
1.14.12.10 | additional information | benzoate induces the expression of benzoate 1,2-dioxygenase | Chromohalobacter sp. | ? | - |
? | |
1.14.13.2 | additional information | p-hydroxybenzoate induces the expression of p-hydroxybenzoate hydroxylase | Chromohalobacter sp. | ? | - |
? | |
1.14.14.92 | benzoate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Chromohalobacter sp. HS-2 | 2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further | ? | |
1.14.14.92 | benzoate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Chromohalobacter sp. HS2 | 2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.2 | p-hydroxybenzoate hydroxylase | - |
Chromohalobacter sp. |
1.14.13.2 | PobA | - |
Chromohalobacter sp. |
1.14.14.92 | benzoate 1,2-dioxygenase | product of genes benA (large subunit), benB (small subunit), benC (electron transfer component) | Chromohalobacter sp. HS-2 |