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Literature summary extracted from

  • Berghaell, S.; Hilditch, S.; Penttilae, M.; Richard, P.
    Identification in the mould Hypocrea jecorina of a gene encoding an NADP(+):D-xylose dehydrogenase (2007), FEMS Microbiol. Lett., 277, 249-253.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.179 gene xyd1, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme in Saccharomyces cerevisiae Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.179 additional information
-
 additional information kinetics, recombinant enzyme Trichoderma reesei
1.1.1.179 5.7
-
 D-glucose pH 8.0, 30°C, recombinant enzyme Trichoderma reesei
1.1.1.179 24
-
 NADP+ pH 8.1, 30°C, recombinant enzyme Trichoderma reesei
1.1.1.179 43
-
 D-xylose pH 8.1, 30°C, recombinant enzyme Trichoderma reesei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.179 Mg2+
-
 Trichoderma reesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.179 42720
-
 x * 42720, sequence calculation Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.179 D-xylose + NADP+ Trichoderma reesei best substrate D-xylono-1,5-lactone + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.179 Trichoderma reesei A8BT09 i.e. Trichoderma reesei, strain VTT-D-80133, gene xyd1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.179 recombinant His-tagged enzyme from Saccharomyces cerevisiae by nickel affinity chromatography Trichoderma reesei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.179 additional information
-
 substrate specificity Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.179 D-galactose + NADP+
-
 Trichoderma reesei D-galactono-1,5-lactone + NADPH
-
 ?
1.1.1.179 D-glucose + NADP+
-
 Trichoderma reesei D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.179 D-ribose + NADP+ low activity Trichoderma reesei ? + NADPH
-
 ?
1.1.1.179 D-xylose + NADP+ best substrate Trichoderma reesei D-xylono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.179 L-arabinose + NADP+
-
 Trichoderma reesei L-arabinono-1,4-lactone + NADPH
-
 ?
1.1.1.179 additional information no or poor activity with D-arabinose, D-mannose, L-rhamnose, D-lyxose, D-fructose, D-glyceraldehyde, and DL-glyceraldehyde Trichoderma reesei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.179 ? x * 42720, sequence calculation Trichoderma reesei

Synonyms

EC Number Synonyms Comment Organism
1.1.1.179 NADP+-dependent D-xylose dehydrogenase
-
 Trichoderma reesei
1.1.1.179 NADP+:D-xylose dehydrogenase
-
 Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.179 30
-
 assay at Trichoderma reesei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.179 5.7
-
 D-glucose pH 8.0, 30°C, recombinant enzyme Trichoderma reesei
1.1.1.179 21.8
-
 D-xylose pH 8.1, 30°C, recombinant enzyme Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.179 8.1
-
 assay at Trichoderma reesei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.179 NADP+ dependent on, specific for Trichoderma reesei