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Literature summary extracted from

  • Hurtado-Guerrero, R.; Raimi, O.; Shepherd, S.; van Aalten, D.M.
    Glucose-6-phosphate as a probe for the glucosamine-6-phosphate N-acetyltransferase Michaelis complex (2007), FEBS Lett., 581, 5597-5600.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.4 using the pseudo-substrate glucose-6-phosphate as a probe with GNA1 crystals, the first GNAT (pseudo-)Michaelis complex is trapped. The complex shows optimal alignment under the ideal Burgi-Dunitz angle for direct nucleophilic attack of the sugar amine, and suggests protonation of the tetrahedral intermediate may proceed through intramolecular proton transfer facilitated by a favourably positioned backbone carbonyl. The hydrogen bond established between the hydroxyl group of Tyr174 and the thioester sulfur increase the electrophilic character of the carbonyl group, favouring the direct nucleophilic attack by the glucosamine 6-phosphate, explaining the 500fold decrease in the turnover when Tyr174 is mutated to Phe, providing direct evidence for the nucleophilic attack of the substrate amine, and giving insight into the protonation of the thiolate leaving group Aspergillus fumigatus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.4 Y174F crystal structure of the mutant Y174F acetyl-CoA/glucose-6-P complex is indistinguishable from the wild-type complex, mutant displays some remaining activity, kcat: 0.076/sec (glucosamine 6-phosphate) Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.4 glucose-6-phosphate weak competitive inhibitor Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.4 0.071
-
glucosamine 6-phosphate
-
Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.4 Aspergillus fumigatus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.4 acetyl-CoA + D-glucosamine 6-phosphate
-
Aspergillus fumigatus CoA + N-acetyl-D-glucosamine 6-phosphate
-
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Synonyms

EC Number Synonyms Comment Organism
2.3.1.4 AfGNA1
-
Aspergillus fumigatus
2.3.1.4 glucosamine-6-phosphate N-acetyltransferase
-
Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.4 additional information
-
assay carried out at room temperature Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.4 0.076
-
glucosamine 6-phosphate mutant Y174F Aspergillus fumigatus
2.3.1.4 38
-
glucosamine 6-phosphate wild-type Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.4 7.5
-
assay at Aspergillus fumigatus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.4 2.6
-
glucose-6-phosphate
-
Aspergillus fumigatus