EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.4 | using the pseudo-substrate glucose-6-phosphate as a probe with GNA1 crystals, the first GNAT (pseudo-)Michaelis complex is trapped. The complex shows optimal alignment under the ideal Burgi-Dunitz angle for direct nucleophilic attack of the sugar amine, and suggests protonation of the tetrahedral intermediate may proceed through intramolecular proton transfer facilitated by a favourably positioned backbone carbonyl. The hydrogen bond established between the hydroxyl group of Tyr174 and the thioester sulfur increase the electrophilic character of the carbonyl group, favouring the direct nucleophilic attack by the glucosamine 6-phosphate, explaining the 500fold decrease in the turnover when Tyr174 is mutated to Phe, providing direct evidence for the nucleophilic attack of the substrate amine, and giving insight into the protonation of the thiolate leaving group | Aspergillus fumigatus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.4 | Y174F | crystal structure of the mutant Y174F acetyl-CoA/glucose-6-P complex is indistinguishable from the wild-type complex, mutant displays some remaining activity, kcat: 0.076/sec (glucosamine 6-phosphate) | Aspergillus fumigatus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.4 | glucose-6-phosphate | weak competitive inhibitor | Aspergillus fumigatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.4 | 0.071 | - |
glucosamine 6-phosphate | - |
Aspergillus fumigatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.4 | Aspergillus fumigatus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.4 | acetyl-CoA + D-glucosamine 6-phosphate | - |
Aspergillus fumigatus | CoA + N-acetyl-D-glucosamine 6-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.4 | AfGNA1 | - |
Aspergillus fumigatus |
2.3.1.4 | glucosamine-6-phosphate N-acetyltransferase | - |
Aspergillus fumigatus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.4 | additional information | - |
assay carried out at room temperature | Aspergillus fumigatus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.4 | 0.076 | - |
glucosamine 6-phosphate | mutant Y174F | Aspergillus fumigatus | |
2.3.1.4 | 38 | - |
glucosamine 6-phosphate | wild-type | Aspergillus fumigatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.4 | 7.5 | - |
assay at | Aspergillus fumigatus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.4 | 2.6 | - |
glucose-6-phosphate | - |
Aspergillus fumigatus |