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Literature summary extracted from
- A novel prokaryotic trans-2-enoyl-CoA reductase from the spirochete Treponema denticola (2007), FEBS Lett., 581, 1561-1566.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.3.1.44 | expressed in Escherichia coli strain BL21(DE3) | Treponema denticola |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.44 | crotonoyl-CoA | - |
Treponema denticola |  |
| 1.3.1.44 | additional information | shows no inhibition by triclosan in a wide range of concentration (0.005-1 mM), or FAD (0.01 mM) | Treponema denticola |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.44 | 0.0027 | - |
crotonoyl-CoA | in potassium phosphate buffer pH 6.2, at 30°C | Treponema denticola | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.44 | 44000 | - |
SDS-PAGE | Treponema denticola |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.44 | Treponema denticola | - |
strain ATCC 35405 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.44 | Ni-NTA agarose column chromatography | Treponema denticola |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.44 | 43 | - |
using crotonoyl-CoA and NADH as substrates, at 30°C | Treponema denticola |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.44 | crotonyl-CoA + NADH + H+ | - |
Treponema denticola | butyryl-CoA + NAD+ | - |
? | |
| 1.3.1.44 | additional information | not active with NADPH or hexenoyl-CoA | Treponema denticola | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.1.44 | monomer | 1 * 44000, SDS-PAGE | Treponema denticola |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.3.1.44 | TER | - |
Treponema denticola |
| 1.3.1.44 | trans-2-enoyl-CoA reductase, NADH-dependent | - |
Treponema denticola |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.44 | NADH | dependent | Treponema denticola | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.44 | 0.000198 | - |
crotonoyl-CoA | in potassium phosphate buffer pH 6.2, at 30°C | Treponema denticola | |
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Release 2023.1 (January 2023)
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