EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.2.3 | expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.2.3 | L56A | less active than the wild type enzyme | Arabidopsis thaliana |
1.3.2.3 | L56C | less active than the wild type enzyme | Arabidopsis thaliana |
1.3.2.3 | L56F | less active than the wild type enzyme | Arabidopsis thaliana |
1.3.2.3 | L56H | less active than the wild type enzyme and releases its FAD cofactor more easily than wild type GALDH | Arabidopsis thaliana |
1.3.2.3 | L56I | the mutant displays a higher turnover rate with L-galactono-1,4-lactone than the wild type enzyme | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.2.3 | L-galactono-1,4-lactone | inhibitory at concentrations above 2 mM | Arabidopsis thaliana | |
1.3.2.3 | additional information | no substrate inhibition with L-gulono-1,4-lactone at concentrations up to 100 mM, D-galactono-1,4-lactone, D-gulono-1,4-lactone, L-mannono-1,4-lactone, and D-galacturonic acid do not inhibit the oxidation of L-galactono-1,4-lactone, no specific inhibition by cations or anions is observed | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.2.3 | 0.026 | - |
phenazine methosulfate | wild type enzyme, in the presence of L-galactono-1,4-lactone, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 0.034 | - |
cytochrome c | wild type enzyme, in the presence of L-galactono-1,4-lactone, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 0.12 | - |
L-galactono-1,4-lactone | mutant enzyme L56H, in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 0.17 | - |
L-galactono-1,4-lactone | wild type enzyme, in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 0.28 | - |
1,4-benzoquinone | wild type enzyme, in the presence of L-galactono-1,4-lactone, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 0.32 | - |
L-galactono-1,4-lactone | mutant enzyme L56I, in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 0.56 | - |
L-galactono-1,4-lactone | mutant enzyme L56F in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 0.99 | - |
L-galactono-1,4-lactone | mutant enzyme L56C, in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 1.7 | - |
L-galactono-1,4-lactone | mutant enzyme L56IA in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 13.1 | - |
L-gulono-1,4-lactone | wild type enzyme, in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.2.3 | mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.2.3 | 55000 | - |
mature enzyme, SDS-PAGE | Arabidopsis thaliana |
1.3.2.3 | 56000 | - |
recombinant His-tagged enzyme, gel filtration | Arabidopsis thaliana |
1.3.2.3 | 58760 | - |
predicted molecular mass of mature His-tagged enzyme | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.2.3 | Arabidopsis thaliana | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.2.3 | Ni-NTA agarose column chromatography and Q-Sepharose column chromatography | Arabidopsis thaliana |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.2.3 | 7 | - |
cell extract, at pH 8.8 | Arabidopsis thaliana |
1.3.2.3 | 76 | - |
after purification, at pH 8.8 | Arabidopsis thaliana |
EC Number | Storage Stability | Organism |
---|---|---|
1.3.2.3 | -80°C, 50 mM sodium phosphate and 300 mM NaCl (pH 7.4), more than 12 months, 30-50% loss of activity | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.2.3 | L-galactono-1,4-lactone + 1,4-benzoquinone | - |
Arabidopsis thaliana | L-ascorbate + reduced 1,4-benzoquinone | - |
? | |
1.3.2.3 | L-galactono-1,4-lactone + cytochrome c | - |
Arabidopsis thaliana | L-ascorbate + reduced cytochrome c | - |
? | |
1.3.2.3 | L-galactono-1,4-lactone + phenazine methosulfate | - |
Arabidopsis thaliana | L-ascorbate + reduced phenazine methosulfate | - |
? | |
1.3.2.3 | L-gulono-1,4-lactone + ferricytochrome c | - |
Arabidopsis thaliana | L-ascorbate + ferrocytochrome c | - |
? | |
1.3.2.3 | additional information | D-galactono-1,4-lactone, D-gulono-1,4-lactone, L-mannono-1,4-lactone, and D-galacturonic acid are no substrates, molecular oxygen can not serve as efficient electron acceptor for the mutant enzymes | Arabidopsis thaliana | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.2.3 | ferricytochrome c oxidoreductase | - |
Arabidopsis thaliana |
1.3.2.3 | GALDH | - |
Arabidopsis thaliana |
1.3.2.3 | L-galactono-gamma-lactone dehydrogenase | catalyzes the final step in the biosynthesis of vitamin C in plants | Arabidopsis thaliana |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.2.3 | 52 | - |
the recombinant enzyme has a half-life of 20 min at 52°C, in the presence of excess FAD the half-life at 52°C is increased to 115 min | Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.2.3 | 4 | - |
L-gulono-1,4-lactone | in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 32 | - |
L-galactono-1,4-lactone | mutant enzyme L56H, in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 45 | - |
L-galactono-1,4-lactone | mutant enzyme L56IA in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 64 | - |
phenazine methosulfate | in the presence of L-galactono-1,4-lactone, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 76 | - |
L-galactono-1,4-lactone | mutant enzyme L56C, in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 108 | - |
1,4-benzoquinone | in the presence of L-galactono-1,4-lactone, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 126 | - |
L-galactono-1,4-lactone | mutant enzyme L56F in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 134 | - |
L-galactono-1,4-lactone | wild type enzyme, in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 151 | - |
cytochrome c | in the presence of L-galactono-1,4-lactone, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana | |
1.3.2.3 | 240 | - |
L-galactono-1,4-lactone | mutant enzyme L56I, in the presence of cytochrome c, in 50 mM sodium phosphate (pH 8.8), at 25°C | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.2.3 | 8.8 | - |
for activity with cytochrome c | Arabidopsis thaliana |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.3.2.3 | 8 | 9.5 | for activity with cytochrome c | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.2.3 | FAD | flavoenzyme with noncovalently bound FAD | Arabidopsis thaliana | |
1.3.2.3 | ferricytochrome c | - |
Arabidopsis thaliana |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.2.3 | 16.4 | - |
L-galactono-1,4-lactone | at 25°C | Arabidopsis thaliana |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.3.2.3 | Arabidopsis thaliana | theoretical value for recombinant His-tagged GALDH | - |
6.8 |