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Literature summary extracted from

  • Michaux, C.; Massant, J.; Kerff, F.; Frere, J.M.; Docquier, J.D.; Vandenberghe, I.; Samyn, B.; Pierrard, A.; Feller, G.; Charlier, P.; Van Beeumen, J.; Wouters, J.
    Crystal structure of a cold-adapted class C beta-lactamase (2008), FEBS J., 275, 1687-1697.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.6 hanging drop vapour diffusion method with 20% poly(ethylene)glycol 6 K in 0.1 M Tris (pH 8.0) Serratia marcescens
3.5.2.6 hanging drop vapour diffusion method with 20% poly(ethylene)glycol 6 K in 0.1 M Tris (pH 8.0) Psychrobacter immobilis
3.5.2.6 hanging drop vapour diffusion method with 20% poly(ethylene)glycol 6 K in 0.1 M Tris (pH 8.0) Pseudomonas fluorescens
3.5.2.6 hanging drop vapour diffusion method with 20% poly(ethylene)glycol 6 K in 0.1 M Tris (pH 8.0) Enterobacter cloacae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.0000005
-
 Cloxacillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae
3.5.2.6 0.00001
-
 ampicillin at 30°C in 50 mM sodium phosphate buffer (pH 7.0) Serratia marcescens
3.5.2.6 0.000118
-
 Oxacillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 0.00016
-
 Cloxacillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 0.0005
-
 benzylpenicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae
3.5.2.6 0.0017
-
 benzylpenicillin at 30°C in 50 mM sodium phosphate buffer (pH 7.0) Serratia marcescens
3.5.2.6 0.0017
-
 benzylpenicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Psychrobacter immobilis
3.5.2.6 0.0023
-
 benzylpenicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 0.0037
-
 carbenicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 0.004
-
 ampicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae
3.5.2.6 0.0051
-
 ampicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 0.006
-
 ampicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Psychrobacter immobilis
3.5.2.6 0.008
-
 cephalexin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Psychrobacter immobilis
3.5.2.6 0.0085
-
 cephalexin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae
3.5.2.6 0.023
-
 nitrocefin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae
3.5.2.6 0.03
-
 cephalexin at 30°C in 50 mM sodium phosphate buffer (pH 7.0) Serratia marcescens
3.5.2.6 0.04
-
 nitrocefin at 30°C in 50 mM sodium phosphate buffer (pH 7.0) Serratia marcescens
3.5.2.6 0.05
-
 cephalexin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 0.051
-
 nitrocefin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Psychrobacter immobilis
3.5.2.6 0.1
-
 cefazolin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Psychrobacter immobilis
3.5.2.6 0.116
-
 nitrocefin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 0.15
-
 cefazolin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 0.54
-
 cefazolin at 30°C in 50 mM sodium phosphate buffer (pH 7.0) Serratia marcescens
3.5.2.6 1.5
-
 cefazolin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 38720
-
 MALDI-TOF mass spectrometry Serratia marcescens
3.5.2.6 38720
-
 MALDI-TOF mass spectrometry Psychrobacter immobilis
3.5.2.6 38720
-
 MALDI-TOF mass spectrometry Pseudomonas fluorescens
3.5.2.6 38720
-
 MALDI-TOF mass spectrometry Enterobacter cloacae

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Enterobacter cloacae P05364
-
-
3.5.2.6 Enterobacter cloacae 908R P05364
-
-
3.5.2.6 Pseudomonas fluorescens P85302
-
-
3.5.2.6 Pseudomonas fluorescens TAE4 P85302
-
-
3.5.2.6 Psychrobacter immobilis
-
-
-
3.5.2.6 Serratia marcescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Serratia marcescens
3.5.2.6
-
 Psychrobacter immobilis
3.5.2.6
-
 Pseudomonas fluorescens
3.5.2.6
-
 Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Serratia marcescens (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Psychrobacter immobilis (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Pseudomonas fluorescens (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Enterobacter cloacae (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Pseudomonas fluorescens TAE4 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Enterobacter cloacae 908R (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Serratia marcescens (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Psychrobacter immobilis (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Pseudomonas fluorescens (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Enterobacter cloacae (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Pseudomonas fluorescens TAE4 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Enterobacter cloacae 908R (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Serratia marcescens (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Psychrobacter immobilis (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Pseudomonas fluorescens (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Enterobacter cloacae (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Pseudomonas fluorescens TAE4 (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Enterobacter cloacae 908R (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefazolin + H2O
-
 Serratia marcescens (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefazolin + H2O
-
 Psychrobacter immobilis (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefazolin + H2O
-
 Pseudomonas fluorescens (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefazolin + H2O
-
 Enterobacter cloacae (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalexin + H2O
-
 Serratia marcescens (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalexin + H2O
-
 Psychrobacter immobilis (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalexin + H2O
-
 Pseudomonas fluorescens (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalexin + H2O
-
 Enterobacter cloacae (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalexin + H2O
-
 Pseudomonas fluorescens TAE4 (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalexin + H2O
-
 Enterobacter cloacae 908R (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cloxacillin + H2O
-
 Serratia marcescens (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cloxacillin + H2O
-
 Psychrobacter immobilis (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cloxacillin + H2O
-
 Pseudomonas fluorescens (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cloxacillin + H2O
-
 Enterobacter cloacae (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cloxacillin + H2O
-
 Pseudomonas fluorescens TAE4 (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cloxacillin + H2O
-
 Enterobacter cloacae 908R (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Serratia marcescens (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Psychrobacter immobilis (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Pseudomonas fluorescens (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Enterobacter cloacae (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 Serratia marcescens (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 Psychrobacter immobilis (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 Pseudomonas fluorescens (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 Enterobacter cloacae (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 class C beta-lactamase
-
 Serratia marcescens
3.5.2.6 class C beta-lactamase
-
 Psychrobacter immobilis
3.5.2.6 class C beta-lactamase
-
 Pseudomonas fluorescens
3.5.2.6 class C beta-lactamase
-
 Enterobacter cloacae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.004
-
 carbenicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae
3.5.2.6 0.004
-
 Cloxacillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae
3.5.2.6 0.008
-
 Oxacillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae
3.5.2.6 0.21
-
 Oxacillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 0.42
-
 Cloxacillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 0.46
-
 ampicillin at 30°C in 50 mM sodium phosphate buffer (pH 7.0) Serratia marcescens
3.5.2.6 0.52
-
 carbenicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 0.53
-
 ampicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae
3.5.2.6 0.65
-
 ampicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 1
-
 ampicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Psychrobacter immobilis
3.5.2.6 18
-
 benzylpenicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae
3.5.2.6 20
-
 benzylpenicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Psychrobacter immobilis
3.5.2.6 26
-
 cephalexin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Psychrobacter immobilis
3.5.2.6 31
-
 cephalexin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 34.3
-
 benzylpenicillin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 61
-
 cefazolin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 72
-
 cephalexin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae
3.5.2.6 75
-
 benzylpenicillin at 30°C in 50 mM sodium phosphate buffer (pH 7.0) Serratia marcescens
3.5.2.6 80
-
 cephalexin at 30°C in 50 mM sodium phosphate buffer (pH 7.0) Serratia marcescens
3.5.2.6 476
-
 cefazolin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Psychrobacter immobilis
3.5.2.6 550
-
 nitrocefin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Pseudomonas fluorescens
3.5.2.6 780
-
 nitrocefin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae
3.5.2.6 1240
-
 nitrocefin at 30°C in 50 mM sodium phosphate buffer (pH 7.0) Serratia marcescens
3.5.2.6 1300
-
 cefazolin at 30°C in 50 mM sodium phosphate buffer (pH 7.0) Serratia marcescens
3.5.2.6 1407
-
 nitrocefin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Psychrobacter immobilis
3.5.2.6 3000
-
 cefazolin at 30°C in 50mM sodium phosphate buffer (pH 7.0) Enterobacter cloacae