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Literature summary extracted from
- Kinetic properties of catecholoxidase activity of tarantula hemocyanin (2008), FEBS J., 275, 1518-1528.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.10.3.1 | SDS | after the addition of SDS, the catecholoxidase activity of Eurypelma hemocyanin develops gradually over a period of 5 min until a constant activity is reached | Aphonopelma californicum (nom. dub.) |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.10.3.1 | The activated state of Eurypelma hemocyanin is stable for several hours. | Aphonopelma californicum (nom. dub.) |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.10.3.1 | azide | typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin | Aphonopelma californicum (nom. dub.) | |
| 1.10.3.1 | dopamine | at high dopamine concentration, a decrease in activity is observed, indicating substrate inhibition | Aphonopelma californicum (nom. dub.) | |
| 1.10.3.1 | kojic acid | typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin | Aphonopelma californicum (nom. dub.) | |
| 1.10.3.1 | L-mimosine | typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin | Aphonopelma californicum (nom. dub.) | |
| 1.10.3.1 | Phenylthiourea | typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin | Aphonopelma californicum (nom. dub.) | |
| 1.10.3.1 | tyramine | typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin | Aphonopelma californicum (nom. dub.) | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.1 | 1.22 | - |
dopamine | at substrate concentrations above 4 mM, substrate inhibition is observed and the data are fitted to a simple model for substrate inhibition. KM, kcat and KI are obtained for the inhibitory binding site | Aphonopelma californicum (nom. dub.) | |
| 1.10.3.1 | 1.45 | - |
dopamine | substrate range 0.3-4 mM | Aphonopelma californicum (nom. dub.) | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.10.3.1 | copper | - |
Aphonopelma californicum (nom. dub.) | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.10.3.1 | Aphonopelma californicum (nom. dub.) | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.10.3.1 | - |
Aphonopelma californicum (nom. dub.) |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.10.3.1 | 2 catechol + O2 | - |
Aphonopelma californicum (nom. dub.) | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.10.3.1 | dopamine + O2 | - |
Aphonopelma californicum (nom. dub.) | dopaminequinone + H2O | - |
? | |
| 1.10.3.1 | epinephrine + O2 | - |
Aphonopelma californicum (nom. dub.) | ? | - |
? | |
| 1.10.3.1 | L-dihydroxyphenylalanine + O2 | L-dopa | Aphonopelma californicum (nom. dub.) | L-dopaquinone + H2O | - |
? | |
| 1.10.3.1 | additional information | to investigate the substrate specificity of activated hemocyanin, the turnover of several diphenols is compared using an oxygen electrode | Aphonopelma californicum (nom. dub.) | ? | - |
? | |
| 1.10.3.1 | N-acetyldopamine + O2 | NADA | Aphonopelma californicum (nom. dub.) | ? | - |
? | |
| 1.10.3.1 | norepinephrine + O2 | - |
Aphonopelma californicum (nom. dub.) | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.10.3.1 | catecholoxidase | - |
Aphonopelma californicum (nom. dub.) |
| 1.10.3.1 | hemocyanin | - |
Aphonopelma californicum (nom. dub.) |
| 1.10.3.1 | phenoloxidase | - |
Aphonopelma californicum (nom. dub.) |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.1 | 5.7 | - |
dopamine | at substrate concentrations above 4 mM, substrate inhibition is observed and the data are fitted to a simple model for substrate inhibition. KM, kcat and KI are obtained for the inhibitory binding site | Aphonopelma californicum (nom. dub.) | |
| 1.10.3.1 | 6 | - |
dopamine | substrate range 0.3-4 mM | Aphonopelma californicum (nom. dub.) | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.1 | 7 | - |
assay at | Aphonopelma californicum (nom. dub.) |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.1 | additional information | - |
additional information | fitting attempts reveal that inhibition by phenylthiourea and azide is not in accordance with a competitive binding model, the binding affinity of the inhibitors can not be determined by fitting the data | Aphonopelma californicum (nom. dub.) | |
| 1.10.3.1 | 0.0119 | - |
kojic acid | with dopamine as a substrate | Aphonopelma californicum (nom. dub.) | |
| 1.10.3.1 | 0.0477 | - |
L-mimosine | with dopamine as a substrate | Aphonopelma californicum (nom. dub.) | |
| 1.10.3.1 | 0.2782 | - |
tyramine | with dopamine as a substrate | Aphonopelma californicum (nom. dub.) | |
| 1.10.3.1 | 29.64 | - |
dopamine | at substrate concentrations above 4 mM, substrate inhibition is observed and the data are fitted to a simple model for substrate inhibition. KM, kcat and KI are obtained for the inhibitory binding site | Aphonopelma californicum (nom. dub.) | |
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