EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.93 | expressed in Escherichia coli strain DH10B | Pseudomonas sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.93 | F375L | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
3.5.1.93 | F375M | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
3.5.1.93 | N266H | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
3.5.1.93 | N266M | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
3.5.1.93 | N266Q | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
3.5.1.93 | N266Q/F375L | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
3.5.1.93 | Y178F/F375H | the mutation synergistically improve the catalytic efficiency towards adipyl-7-ADCA 36fold, The activity of this double mutant towards adipyl-7-ADCA is 50% of the activity of the wild-type enzyme towards the preferred substrate glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. |
3.5.1.93 | Y178H | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
3.5.1.93 | Y178H/N266H | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
3.5.1.93 | Y178H/N266M | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
3.5.1.93 | Y178H/N266Q/F375L | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.93 | 0.04 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266H | Pseudomonas sp. | |
3.5.1.93 | 0.042 | - |
glutaryl-7-aminocephalosporanic acid | wild type enzyme | Pseudomonas sp. | |
3.5.1.93 | 0.08 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H | Pseudomonas sp. | |
3.5.1.93 | 0.08 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H/N266H | Pseudomonas sp. | |
3.5.1.93 | 0.08 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H/N266Q/F375L | Pseudomonas sp. | |
3.5.1.93 | 0.12 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266Q | Pseudomonas sp. | |
3.5.1.93 | 0.14 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266H | Pseudomonas sp. | |
3.5.1.93 | 0.17 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266M | Pseudomonas sp. | |
3.5.1.93 | 0.17 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme F375M | Pseudomonas sp. | |
3.5.1.93 | 0.17 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266Q/F375L | Pseudomonas sp. | |
3.5.1.93 | 0.17 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266Q/F375L | Pseudomonas sp. | |
3.5.1.93 | 0.17 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H/N266M | Pseudomonas sp. | |
3.5.1.93 | 0.19 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H/N266H | Pseudomonas sp. | |
3.5.1.93 | 0.26 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme F375L | Pseudomonas sp. | |
3.5.1.93 | 0.28 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H/N266Q/F375L | Pseudomonas sp. | |
3.5.1.93 | 0.3 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H/N266M | Pseudomonas sp. | |
3.5.1.93 | 0.33 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266M | Pseudomonas sp. | |
3.5.1.93 | 0.47 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H | Pseudomonas sp. | |
3.5.1.93 | 0.62 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266Q | Pseudomonas sp. | |
3.5.1.93 | 0.65 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme F375M | Pseudomonas sp. | |
3.5.1.93 | 0.7 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme F375L | Pseudomonas sp. | |
3.5.1.93 | 0.8 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | wild type enzyme | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.93 | Pseudomonas sp. | - |
- |
- |
3.5.1.93 | Pseudomonas sp. SY-77 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.93 | - |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.93 | adipyl-7-aminodesacetoxycephalosporanic acid + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.1.93 | adipyl-7-aminodesacetoxycephalosporanic acid + H2O | - |
Pseudomonas sp. SY-77 | ? | - |
? | |
3.5.1.93 | cephalosporin C + H2O | - |
Pseudomonas sp. | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
3.5.1.93 | cephalosporin C + H2O | - |
Pseudomonas sp. SY-77 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
3.5.1.93 | glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. | 7-aminocephalosporanate + glutarate | - |
? | |
3.5.1.93 | glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. SY-77 | 7-aminocephalosporanate + glutarate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.93 | adipyl-cephalosporin acylase | - |
Pseudomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.93 | 0.38 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | wild type enzyme | Pseudomonas sp. | |
3.5.1.93 | 0.46 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266Q | Pseudomonas sp. | |
3.5.1.93 | 0.47 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266H | Pseudomonas sp. | |
3.5.1.93 | 0.48 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266Q/F375L | Pseudomonas sp. | |
3.5.1.93 | 0.65 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme F375M | Pseudomonas sp. | |
3.5.1.93 | 0.66 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H | Pseudomonas sp. | |
3.5.1.93 | 0.66 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H/N266Q/F375L | Pseudomonas sp. | |
3.5.1.93 | 0.67 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme F375L | Pseudomonas sp. | |
3.5.1.93 | 0.75 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266Q/F375L | Pseudomonas sp. | |
3.5.1.93 | 0.89 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H/N266M | Pseudomonas sp. | |
3.5.1.93 | 0.95 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H/N266H | Pseudomonas sp. | |
3.5.1.93 | 1.1 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme F375L | Pseudomonas sp. | |
3.5.1.93 | 1.2 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H/N266M | Pseudomonas sp. | |
3.5.1.93 | 1.8 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266M | Pseudomonas sp. | |
3.5.1.93 | 1.9 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266M | Pseudomonas sp. | |
3.5.1.93 | 2.02 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme F375M | Pseudomonas sp. | |
3.5.1.93 | 2.2 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H/N266H | Pseudomonas sp. | |
3.5.1.93 | 2.4 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266Q | Pseudomonas sp. | |
3.5.1.93 | 2.5 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H | Pseudomonas sp. | |
3.5.1.93 | 2.5 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H/N266Q/F375L | Pseudomonas sp. | |
3.5.1.93 | 3.1 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266H | Pseudomonas sp. | |
3.5.1.93 | 4.1 | - |
glutaryl-7-aminocephalosporanic acid | wild type enzyme | Pseudomonas sp. |