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Literature summary extracted from

  • Rustler, S.; Mueller, A.; Windeisen, V.; Chmura, A.; Fernandes, B.C.; Kiziak, C.; Stolz, A.
    Conversion of mandelonitrile and phenylglycinenitrile by recombinant E. coli cells synthesizing a nitrilase from Pseudomonas fluorescens EBC191 (2007), Enzyme Microb. Technol., 40, 598-606.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expressed in Escherichia coli strain JM109(pIK9) Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Pseudomonas fluorescens
-
-
-
3.5.5.1 Pseudomonas fluorescens EBC191
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 mandelonitrile + 2 H2O
-
 Pseudomonas fluorescens mandelic acid + NH3
-
 ?
3.5.5.1 mandelonitrile + 2 H2O
-
 Pseudomonas fluorescens EBC191 mandelic acid + NH3
-
 ?
3.5.5.1 phenylglycinonitrile + H2O
-
 Pseudomonas fluorescens phenylglycine + NH3
-
 ?
3.5.5.1 phenylglycinonitrile + H2O
-
 Pseudomonas fluorescens EBC191 phenylglycine + NH3
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 5
-
-
 Pseudomonas fluorescens