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Literature summary extracted from

  • Payandeh, J.; Pai, E.F.
    A structural basis for Mg2+ homeostasis and the CorA translocation cycle (2006), EMBO J., 25, 3762-3773.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.2.2.14 free wild-type CorA, CorA1-351, and SeMet-AfCorA1-263, in complex with several different metal ions, 15 mg/ml purified CorA1-351 in 20 mM Tris pH 8.0, 100 mM NaCl, 1 mM TCEP, with or without 0.026% n-dodecyl-beta-D-maltoside, with precipitant vapor-diffusion over 20% PEG 400, 0.2 M CaCl2, 0.1 M HEPES, pH 7.0, 40 mg/ml native and SeMet-AfCorA1-263 from 1.3 M ammonium sulfate, 10 mM CoCl2, 0.1 M HEPES, pH 7.4, X-ray diffraction structure determination and analysis at 3.7 A resolution Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.2.14 ATP + H2O + Mg2+/out Thermotoga maritima
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 ADP + phosphate + Mg2+/in
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.14 Thermotoga maritima Q9WZ31 gene corA
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.14 ATP + H2O + Mg2+/out
-
 Thermotoga maritima ADP + phosphate + Mg2+/in
-
 ?
7.2.2.14 ATP + H2O + Mg2+/out One metal binds near the universally conserved GMN motif, apparently stabilized within the transmembrane region discriminating between the size and preferred coordination geometry of hydrated substrates. CorA achieves specificity by requiring the sequential dehydration of substrates. Ten metal sites are located within the cytoplasmic funnel domain and linked to long extensions of the pore helices regulating the transport status of CorA, this region is an intrinsic divalent cation sensor functioning as a Mg2+-specific homeostatic molecular switch, overview Thermotoga maritima ADP + phosphate + Mg2+/in
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 ?

Subunits

EC Number Subunits Comment Organism
7.2.2.14 More structural basis for Mg2+ homeostasis and the CorA translocation cycle, open state model and two-state model, overview Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
7.2.2.14 CorA
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 Thermotoga maritima
7.2.2.14 CorA Mg2þ transporter homologue
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 Thermotoga maritima